{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:15:04Z","timestamp":1790792104302,"version":"4.1.0"},"reference-count":114,"publisher":"Oxford University Press (OUP)","issue":"19","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03DE028103"],"award-info":[{"award-number":["R03DE028103"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03DE027393"],"award-info":[{"award-number":["R03DE027393"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01LM012806"],"award-info":[{"award-number":["R01LM012806"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE026767"],"award-info":[{"award-number":["R01DE026767"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE029818"],"award-info":[{"award-number":["R01DE029818"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03DE026509"],"award-info":[{"award-number":["R03DE026509"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The etiology of cleft lip with\/without cleft palate (CL\/P), one of the most frequent craniofacial birth defects worldwide, is complicated by contributions of both genetic and environmental factors. Understanding the etiology of these conditions is essential for developing preventive strategies. This study thus aims to identify regulatory networks of microRNAs (miRNAs), transcriptional factors (TFs) and non-TF genes associated with cleft lip (CL) that are conserved in humans and mice. Notably, we found that miR-27b, miR-133b, miR-205, miR-376b and miR-376c were involved in the regulation of CL-associated gene expression in both humans and mice. Among the candidate miRNAs, the overexpression of miR-27b, miR-133b and miR-205, but not miR-376b and miR-376c, significantly inhibited cell proliferation through suppression of CL-associated genes (miR-27b suppressed PAX9 and RARA; miR-133b suppressed FGFR1, PAX7, and SUMO1; and miR-205 suppressed PAX9 and RARA) in cultured human and mouse lip mesenchymal cells. Taken together, our results suggest that elevated expression of miR-27b, miR-133b and miR-205 may play a crucial role in CL through the suppression of genes associated with CL.<\/jats:p>","DOI":"10.1093\/hmg\/ddab151","type":"journal-article","created":{"date-parts":[[2021,6,5]],"date-time":"2021-06-05T19:11:04Z","timestamp":1622920264000},"page":"1881-1893","source":"Crossref","is-referenced-by-count":13,"title":["Identification of microRNAs and gene regulatory networks in cleft lip common in humans and mice"],"prefix":"10.1093","volume":"30","author":[{"given":"Hiroki","family":"Yoshioka","sequence":"first","affiliation":[{"name":"Department of Diagnostic & Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA"},{"name":"Center for Craniofacial Research, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimin","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Precision Health, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Diagnostic & Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA"},{"name":"Center for Craniofacial Research, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai Shankar","family":"Ramakrishnan","sequence":"additional","affiliation":[{"name":"Department of Diagnostic & Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA"},{"name":"Center for Craniofacial Research, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Precision Health, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"},{"name":"Human Genetics Center, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"},{"name":"MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3975-6836","authenticated-orcid":false,"given":"Junichi","family":"Iwata","sequence":"additional","affiliation":[{"name":"Department of Diagnostic & Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA"},{"name":"Center for Craniofacial Research, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA"},{"name":"MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,6,8]]},"reference":[{"key":"2021091611515014700_ref1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1093\/ejo\/26.1.7","article-title":"The complex genetics of cleft lip and palate","volume":"26","author":"Cobourne","year":"2004","journal-title":"Eur. J. Orthod."},{"key":"2021091611515014700_ref2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1080\/14767050500072854","article-title":"Are isolated facial cleft lip and palate associated with increased perinatal mortality? A cohort study from the West Midlands region, 1995-1997","volume":"17","author":"Ngai","year":"2005","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"2021091611515014700_ref3","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1002\/bdra.23320","article-title":"Epigenetic mechanism causes Wnt9b deficiency and nonsyndromic cleft lip and palate in the A\/WySn mouse strain","volume":"100","author":"Juriloff","year":"2014","journal-title":"Birth Defects Res A Clin. Mol. Teratol."},{"key":"2021091611515014700_ref4","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1097\/SCS.0b013e31822e5e4d","article-title":"Genetics of syndromic and nonsyndromic cleft lip and palate","volume":"22","author":"Stuppia","year":"2011","journal-title":"J. Craniofac. Surg."},{"key":"2021091611515014700_ref5","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1016\/S0140-6736(09)60695-4","article-title":"Cleft lip and palate","volume":"374","author":"Mossey","year":"2009","journal-title":"Lancet"},{"key":"2021091611515014700_ref6","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1002\/dvdy.20646","article-title":"Development of the upper lip: morphogenetic and molecular mechanisms","volume":"235","author":"Jiang","year":"2006","journal-title":"Dev. Dyn"},{"key":"2021091611515014700_ref7","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1242\/dev.116.2.297","article-title":"Vital dye analysis of cranial neural crest cell migration in the mouse embryo","volume":"116","author":"Serbedzija","year":"1992","journal-title":"Development"},{"key":"2021091611515014700_ref8","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1006\/dbio.1994.1211","article-title":"The contribution of both forebrain and midbrain crest cells to the mesenchyme in the frontonasal mass of mouse embryos","volume":"164","author":"Osumi-Yamashita","year":"1994","journal-title":"Dev. Biol."},{"key":"2021091611515014700_ref9","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1242\/dev.075796","article-title":"Wnt9b-dependent FGF signaling is crucial for outgrowth of the nasal and maxillary processes during upper jaw and lip development","volume":"139","author":"Jin","year":"2012","journal-title":"Development"},{"key":"2021091611515014700_ref10","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/j.1469-7580.2005.00485.x","article-title":"Patterning the neural crest derivatives during development of the vertebrate head: insights from avian studies","volume":"207","author":"Creuzet","year":"2005","journal-title":"J. Anat."},{"key":"2021091611515014700_ref11","doi-asserted-by":"crossref","first-page":"3161","DOI":"10.1242\/dev.037440","article-title":"Lrp6-mediated canonical Wnt signaling is required for lip formation and fusion","volume":"136","author":"Song","year":"2009","journal-title":"Development"},{"issue":"1159","key":"2021091611515014700_ref12","first-page":"1161","article-title":"Genomics","volume":"337","author":"Pennisi","year":"2012","journal-title":"ENCODE project writes eulogy for junk DNA. Science"},{"key":"2021091611515014700_ref13","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1038\/35103511","article-title":"Non-coding RNA genes and the modern RNA world","volume":"2","author":"Eddy","year":"2001","journal-title":"Nat. Rev. Genet."},{"key":"2021091611515014700_ref14","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/s41576-019-0184-5","article-title":"Regulation of gene expression by cis-acting long non-coding RNAs","volume":"21","author":"Gil","year":"2020","journal-title":"Nat. Rev. Genet."},{"key":"2021091611515014700_ref15","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.cbpa.2015.10.024","article-title":"RNA epigenetics--chemical messages for posttranscriptional gene regulation","volume":"30","author":"Roundtree","year":"2016","journal-title":"Curr. Opin. Chem. Biol."},{"key":"2021091611515014700_ref16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nature11247","article-title":"An integrated encyclopedia of DNA elements in the human genome","volume":"489","author":"Consortium","year":"2012","journal-title":"Nature"},{"key":"2021091611515014700_ref17","doi-asserted-by":"crossref","first-page":"4429","DOI":"10.3390\/ijms16034429","article-title":"Mammalian introns: when the junk generates molecular diversity","volume":"16","author":"Hube","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"2021091611515014700_ref18","doi-asserted-by":"crossref","first-page":"e1002384","DOI":"10.1371\/journal.pgen.1002384","article-title":"Repetitive elements may comprise over two-thirds of the human genome","volume":"7","author":"Koning","year":"2011","journal-title":"PLoS Genet."},{"key":"2021091611515014700_ref19","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.tig.2007.02.006","article-title":"Which transposable elements are active in the human genome?","volume":"23","author":"Mills","year":"2007","journal-title":"Trends Genet."},{"key":"2021091611515014700_ref20","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/nrg3117","article-title":"Repetitive DNA and next-generation sequencing: computational challenges and solutions","volume":"13","author":"Treangen","year":"2011","journal-title":"Nat. Rev. Genet."},{"key":"2021091611515014700_ref21","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/nrg2072","article-title":"Transposable elements and the epigenetic regulation of the genome","volume":"8","author":"Slotkin","year":"2007","journal-title":"Nat. Rev. Genet."},{"key":"2021091611515014700_ref22","doi-asserted-by":"crossref","first-page":"e101112","DOI":"10.15252\/embj.2018101112","article-title":"RNA toxicity in non-coding repeat expansion disorders","volume":"39","author":"Swinnen","year":"2020","journal-title":"EMBO J."},{"key":"2021091611515014700_ref23","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1038\/nrg.2017.115","article-title":"Tandem repeats mediating genetic plasticity in health and disease","volume":"19","author":"Hannan","year":"2018","journal-title":"Nat. Rev. Genet."},{"key":"2021091611515014700_ref24","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.dnarep.2015.11.008","article-title":"Disease-associated repeat instability and mismatch repair","volume":"38","author":"Schmidt","year":"2016","journal-title":"DNA repair"},{"key":"2021091611515014700_ref25","doi-asserted-by":"crossref","first-page":"762","DOI":"10.18632\/aging.100085","article-title":"Coupling transcriptional and post-transcriptional miRNA regulation in the control of cell fate","volume":"1","author":"Shalgi","year":"2009","journal-title":"Aging"},{"key":"2021091611515014700_ref26","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1038\/nrm2868","article-title":"MicroRNA control of signal transduction","volume":"11","author":"Inui","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021091611515014700_ref27","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/nrm3838","article-title":"Regulation of microRNA biogenesis","volume":"15","author":"Ha","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021091611515014700_ref28","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/s41580-018-0059-1","article-title":"Regulation of microRNA biogenesis and its crosstalk with other cellular pathways","volume":"20","author":"Treiber","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021091611515014700_ref29","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1101\/gad.974702","article-title":"miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs","volume":"16","author":"Mourelatos","year":"2002","journal-title":"Genes Dev."},{"key":"2021091611515014700_ref30","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.tibs.2010.03.009","article-title":"Making RISC","volume":"35","author":"Kawamata","year":"2010","journal-title":"Trends Biochem. Sci."},{"key":"2021091611515014700_ref31","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/nrg3965","article-title":"Towards a molecular understanding of microRNA-mediated gene silencing","volume":"16","author":"Jonas","year":"2015","journal-title":"Nat. Rev. Genet."},{"key":"2021091611515014700_ref32","doi-asserted-by":"crossref","first-page":"4653","DOI":"10.1242\/dev.02073","article-title":"MicroRNA functions in animal development and human disease","volume":"132","author":"Alvarez-Garcia","year":"2005","journal-title":"Development"},{"key":"2021091611515014700_ref33","doi-asserted-by":"crossref","first-page":"2570","DOI":"10.3892\/etm.2017.4248","article-title":"A pilot study: screening target miRNAs in tissue of nonsyndromic cleft lip with or without cleft palate","volume":"13","author":"Wang","year":"2017","journal-title":"Exp. Ther. Med."},{"key":"2021091611515014700_ref34","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1002\/bdra.20684","article-title":"Developmental microRNA expression profiling of murine embryonic orofacial tissue","volume":"88","author":"Mukhopadhyay","year":"2010","journal-title":"Birth Defects Res. Part A Clin. Mol. Teratol."},{"key":"2021091611515014700_ref35","doi-asserted-by":"crossref","first-page":"789","DOI":"10.2337\/db12-0365","article-title":"The Wnt signaling pathway effector TCF7L2 controls gut and brain proglucagon gene expression and glucose homeostasis","volume":"62","author":"Shao","year":"2013","journal-title":"Diabetes"},{"key":"2021091611515014700_ref36","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/dgd.12140","article-title":"MicroRNA expression profiling of the developing murine upper lip","volume":"56","author":"Warner","year":"2014","journal-title":"Develop. Growth Differ."},{"key":"2021091611515014700_ref37","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.ijporl.2018.06.005","article-title":"Identification of key genes in cleft lip with or without cleft palate regulated by miR-199a-5p","volume":"111","author":"Chen","year":"2018","journal-title":"Int. J. Pediatr. Otorhinolaryngol."},{"key":"2021091611515014700_ref38","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.biopha.2016.05.033","article-title":"Expression profile of plasma microRNAs in nonsyndromic cleft lip and their clinical significance as biomarkers","volume":"82","author":"Zou","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"2021091611515014700_ref39","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1111\/odi.12741","article-title":"Genetic variants of microRNA processing genes and risk of non-syndromic orofacial clefts","volume":"24","author":"Xu","year":"2018","journal-title":"Oral Dis."},{"key":"2021091611515014700_ref40","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1101\/gad.1953310","article-title":"Canonical and alternate functions of the microRNA biogenesis machinery","volume":"24","author":"Chong","year":"2010","journal-title":"Genes Dev."},{"key":"2021091611515014700_ref41","doi-asserted-by":"crossref","first-page":"9408","DOI":"10.1523\/JNEUROSCI.2700-12.2013","article-title":"Reduced adult hippocampal neurogenesis and working memory deficits in the Dgcr8-deficient mouse model of 22q11.2 deletion-associated schizophrenia can be rescued by IGF2","volume":"33","author":"Ouchi","year":"2013","journal-title":"J. Neurosci."},{"key":"2021091611515014700_ref42","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/ng.138","article-title":"Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model","volume":"40","author":"Stark","year":"2008","journal-title":"Nat. Genet."},{"key":"2021091611515014700_ref43","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ydbio.2011.11.008","article-title":"Dgcr8 controls neural crest cells survival in cardiovascular development","volume":"362","author":"Chapnik","year":"2012","journal-title":"Dev. Biol."},{"key":"2021091611515014700_ref44","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.ydbio.2010.01.039","article-title":"Dicer is required for survival of differentiating neural crest cells","volume":"340","author":"Zehir","year":"2010","journal-title":"Dev. Biol."},{"key":"2021091611515014700_ref45","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.mod.2010.12.002","article-title":"Dicer activity in neural crest cells is essential for craniofacial organogenesis and pharyngeal arch artery morphogenesis","volume":"128","author":"Nie","year":"2011","journal-title":"Mech. Dev."},{"key":"2021091611515014700_ref46","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1093\/jmcb\/mjq008","article-title":"Wnt1-cre-mediated conditional loss of Dicer results in malformation of the midbrain and cerebellum and failure of neural crest and dopaminergic differentiation in mice","volume":"2","author":"Huang","year":"2010","journal-title":"J. Mol. Cell Biol."},{"key":"2021091611515014700_ref47","doi-asserted-by":"crossref","first-page":"310","DOI":"10.4103\/0971-6866.107984","article-title":"Conditional deletion of the human ortholog gene Dicer1 in Pax2-Cre expression domain impairs orofacial development","volume":"18","author":"Barritt","year":"2012","journal-title":"Indian journal of human genetics"},{"key":"2021091611515014700_ref48","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1073\/pnas.0810766105","article-title":"DGCR8-dependent microRNA biogenesis is essential for skin development","volume":"106","author":"Yi","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021091611515014700_ref49","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.ydbio.2010.01.019","article-title":"Tooth morphogenesis and ameloblast differentiation are regulated by micro-RNAs","volume":"340","author":"Michon","year":"2010","journal-title":"Dev. Biol."},{"key":"2021091611515014700_ref50","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1002\/dvdy.23828","article-title":"Distinct roles of microRNAs in epithelium and mesenchyme during tooth development","volume":"241","author":"Oommen","year":"2012","journal-title":"Dev. Dyn."},{"key":"2021091611515014700_ref51","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.tibs.2004.07.001","article-title":"Common logic of transcription factor and microRNA action","volume":"29","author":"Hobert","year":"2004","journal-title":"Trends Biochem. Sci."},{"key":"2021091611515014700_ref52","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.molcel.2007.06.017","article-title":"MicroRNA targeting specificity in mammals: determinants beyond seed pairing","volume":"27","author":"Grimson","year":"2007","journal-title":"Mol. Cell"},{"key":"2021091611515014700_ref53","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1126\/science.1151651","article-title":"Gene regulation by transcription factors and microRNAs","volume":"319","author":"Hobert","year":"2008","journal-title":"Science"},{"key":"2021091611515014700_ref54","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1752-0509-4-10","article-title":"A novel microRNA and transcription factor mediated regulatory network in schizophrenia","volume":"4","author":"Guo","year":"2010","journal-title":"BMC Syst. Biol."},{"key":"2021091611515014700_ref55","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1261\/rna.042754.113","article-title":"Reproducible combinatorial regulatory networks elucidate novel oncogenic microRNAs in non-small cell lung cancer","volume":"20","author":"Mitra","year":"2014","journal-title":"RNA"},{"key":"2021091611515014700_ref56","doi-asserted-by":"crossref","first-page":"e1002488","DOI":"10.1371\/journal.pcbi.1002488","article-title":"Uncovering microRNA and transcription factor mediated regulatory networks in glioblastoma","volume":"8","author":"Sun","year":"2012","journal-title":"PLoS Comput. Biol."},{"key":"2021091611515014700_ref57","first-page":"53","article-title":"Dexamethasone-induced inhibition of miR-132 via methylation promotes TGF-beta-driven progression of pancreatic cancer","volume":"54","author":"Abukiwan","year":"2019","journal-title":"Int. J. Oncol."},{"key":"2021091611515014700_ref58","first-page":"16","article-title":"Network-based identification of critical regulators as putative drivers of human cleft lip","volume":"12","author":"Li","year":"2019","journal-title":"BMC Med. Genet."},{"key":"2021091611515014700_ref59","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1186\/s12864-019-6238-4","article-title":"MicroRNA-124-3p suppresses mouse lip mesenchymal cell proliferation through the regulation of genes associated with cleft lip in the mouse","volume":"20","author":"Suzuki","year":"2019","journal-title":"BMC Genomics"},{"key":"2021091611515014700_ref60","first-page":"70","article-title":"MicroRNA-655-3p and microRNA-497-5p inhibit cell proliferation in cultured human lip cells through the regulation of genes related to human cleft lip","volume":"12","author":"Gajera","year":"2019","journal-title":"BMC Med. Genet."},{"key":"2021091611515014700_ref61","doi-asserted-by":"crossref","first-page":"D68","DOI":"10.1093\/nar\/gkt1181","article-title":"miRBase: annotating high confidence microRNAs using deep sequencing data","volume":"42","author":"Kozomara","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"2021091611515014700_ref62","doi-asserted-by":"crossref","first-page":"D23","DOI":"10.1093\/nar\/gky1069","article-title":"Database resources of the National Center for Biotechnology Information","volume":"47","author":"Sayers","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2021091611515014700_ref63","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang","year":"2008","journal-title":"Nat. Protoc."},{"key":"2021091611515014700_ref64","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.devcel.2008.06.004","article-title":"Ectodermal Smad4 and p38 MAPK are functionally redundant in mediating TGF-\u03b2\/BMP signaling during tooth and palate development","volume":"15","author":"Xu","year":"2008","journal-title":"Dev. Cell"},{"key":"2021091611515014700_ref65","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1002\/jcb.23457","article-title":"MiR-17-92 cluster regulates cell proliferation and collagen synthesis by targeting TGFB pathway in mouse palatal mesenchymal cells","volume":"113","author":"Li","year":"2012","journal-title":"J. Cell. Biochem."},{"key":"2021091611515014700_ref66","doi-asserted-by":"crossref","first-page":"2353","DOI":"10.1074\/jbc.M111.280974","article-title":"Fibroblast growth factor 9 (FGF9)-pituitary homeobox 2 (PITX2) pathway mediates transforming growth factor \u03b2 (TGF\u03b2) signaling to regulate cell proliferation in palatal mesenchyme during mouse palatogenesis","volume":"287","author":"Iwata","year":"2012","journal-title":"J. Biol. Chem."},{"key":"2021091611515014700_ref67","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s00418-011-0876-1","article-title":"MiR-200b is involved in Tgf-\u03b2 signaling to regulate mammalian palate development","volume":"137","author":"Shin","year":"2012","journal-title":"Histochem. Cell Biol."},{"key":"2021091611515014700_ref68","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/nrg2933","article-title":"Cleft lip and palate: understanding genetic and environmental influences","volume":"12","author":"Dixon","year":"2011","journal-title":"Nat. Rev. Genet."},{"key":"2021091611515014700_ref69","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1186\/1752-0509-8-S4-S11","article-title":"cytoHubba: identifying hub objects and sub-networks from complex interactome","volume":"8","author":"Chin","year":"2014","journal-title":"BMC Syst. Biol."},{"key":"2021091611515014700_ref70","doi-asserted-by":"crossref","first-page":"W130","DOI":"10.1093\/nar\/gkx356","article-title":"WebGestalt 2017: a more comprehensive, powerful, flexible and interactive gene set enrichment analysis toolkit","volume":"45","author":"Wang","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"2021091611515014700_ref71","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1093\/hmg\/ddv047","article-title":"A single nucleotide polymorphism associated with isolated cleft lip and palate, thyroid cancer and hypothyroidism alters the activity of an oral epithelium and thyroid enhancer near FOXE1","volume":"24","author":"Lidral","year":"2015","journal-title":"Hum. Mol. Genet."},{"key":"2021091611515014700_ref72","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1093\/hmg\/ddn121","article-title":"Variation in WNT genes is associated with non-syndromic cleft lip with or without cleft palate","volume":"17","author":"Chiquet","year":"2008","journal-title":"Hum. Mol. Genet."},{"key":"2021091611515014700_ref73","doi-asserted-by":"crossref","first-page":"111415","DOI":"10.1016\/j.ecoenv.2020.111415","article-title":"Hypermethylation of WNT3A gene and non-syndromic cleft lip and\/or palate in association with in utero exposure to lead: a mediation analysis","volume":"208","author":"Yang","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"2021091611515014700_ref74","doi-asserted-by":"crossref","first-page":"D109","DOI":"10.1093\/nar\/gkr988","article-title":"KEGG for integration and interpretation of large-scale molecular data sets","volume":"40","author":"Kanehisa","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"2021091611515014700_ref75","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1093\/nar\/30.7.1575","article-title":"An efficient algorithm for large-scale detection of protein families","volume":"30","author":"Enright","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"2021091611515014700_ref76","first-page":"8481","article-title":"Selection of key genes and regulators associated with Treacher Collins syndrome based on expression profiling analysis","volume":"10","author":"Xu","year":"2017","journal-title":"Int. J. Clin. Exp. Med."},{"key":"2021091611515014700_ref77","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3390\/jdb7020009","article-title":"Hedgehog signaling and embryonic craniofacial disorders","volume":"7","author":"Abramyan","year":"2019","journal-title":"J. Dev. Biol."},{"key":"2021091611515014700_ref78","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1093\/bib\/bbz082","article-title":"Critical microRNAs and regulatory motifs in cleft palate identified by a conserved miRNA-TF-gene network approach in humans and mice","volume":"21","author":"Li","year":"2020","journal-title":"Brief. Bioinform."},{"key":"2021091611515014700_ref79","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1177\/0022034518811861","article-title":"The function and regulatory network of Pax9 gene in palate development","volume":"98","author":"Li","year":"2019","journal-title":"J. Dent. Res."},{"key":"2021091611515014700_ref80","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3389\/fphys.2016.00281","article-title":"MicroRNA profiling during craniofacial development: potential roles for Mir23b and Mir133b","volume":"7","author":"Ding","year":"2016","journal-title":"Front. Physiol."},{"key":"2021091611515014700_ref81","first-page":"93","article-title":"MicroRNA-374a, \u22124680, and -133b suppress cell proliferation through the regulation of genes associated with human cleft palate in cultured human palate cells","volume":"12","author":"Suzuki","year":"2019","journal-title":"BMC Med. Genet."},{"key":"2021091611515014700_ref82","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.biopha.2018.04.026","article-title":"A review of FGF signaling in palate development","volume":"103","author":"Weng","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"2021091611515014700_ref83","doi-asserted-by":"crossref","first-page":"22174","DOI":"10.1074\/jbc.M113.463620","article-title":"Type 1 fibroblast growth factor receptor in cranial neural crest cell-derived mesenchyme is required for palatogenesis","volume":"288","author":"Wang","year":"2013","journal-title":"J. Biol. Chem."},{"key":"2021091611515014700_ref84","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1597\/11-132","article-title":"The FGF and FGFR gene family and risk of cleft lip with or without cleft palate","volume":"50","author":"Wang","year":"2013","journal-title":"Cleft Palate Craniofac. J."},{"key":"2021091611515014700_ref85","doi-asserted-by":"crossref","first-page":"4512","DOI":"10.1073\/pnas.0607956104","article-title":"Impaired FGF signaling contributes to cleft lip and palate","volume":"104","author":"Riley","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2021091611515014700_ref86","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1242\/dev.122.3.831","article-title":"Dysgenesis of cephalic neural crest derivatives in Pax7\u2212\/\u2212 mutant mice","volume":"122","author":"Mansouri","year":"1996","journal-title":"Development"},{"key":"2021091611515014700_ref87","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1111\/odi.13139","article-title":"PAX7 nucleotide variants and the risk of non-syndromic orofacial clefts in the polish population","volume":"25","author":"Gaczkowska","year":"2019","journal-title":"Oral Dis."},{"key":"2021091611515014700_ref88","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.tig.2007.09.002","article-title":"FGF signalling and SUMO modification: new players in the aetiology of cleft lip and\/or palate","volume":"23","author":"Pauws","year":"2007","journal-title":"Trends Genet."},{"key":"2021091611515014700_ref89","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1089\/gtmb.2014.0056","article-title":"SUMO1 genetic polymorphisms may contribute to the risk of nonsyndromic cleft lip with or without palate: a meta-analysis","volume":"18","author":"Tang","year":"2014","journal-title":"Genet. Test. Mol. Biomarkers"},{"key":"2021091611515014700_ref90","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1089\/dna.2010.1110","article-title":"Interactions between small ubiquitin-like modifier 1 and nonsyndromic orofacial clefts","volume":"30","author":"Jia","year":"2011","journal-title":"DNA Cell Biol."},{"key":"2021091611515014700_ref91","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1089\/dna.2010.1034","article-title":"Association between polymorphisms at small ubiquitin-like modifier 1 and nonsyndromic orofacial clefts in Western China","volume":"29","author":"Jia","year":"2010","journal-title":"DNA Cell Biol."},{"key":"2021091611515014700_ref92","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/j.bbrc.2008.10.138","article-title":"SUMO1 polymorphisms are associated with non-syndromic cleft lip with or without cleft palate","volume":"377","author":"Song","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021091611515014700_ref93","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1126\/science.1128406","article-title":"SUMO1 haploinsufficiency leads to cleft lip and palate","volume":"313","author":"Alkuraya","year":"2006","journal-title":"Science"},{"key":"2021091611515014700_ref94","doi-asserted-by":"crossref","first-page":"4106","DOI":"10.1242\/jcs.038570","article-title":"Loss of SUMO1 in mice affects RanGAP1 localization and formation of PML nuclear bodies, but is not lethal as it can be compensated by SUMO2 or SUMO3","volume":"121","author":"Evdokimov","year":"2008","journal-title":"J. Cell Sci."},{"key":"2021091611515014700_ref95","doi-asserted-by":"crossref","first-page":"5381","DOI":"10.1128\/MCB.00651-08","article-title":"Sumo-1 function is dispensable in normal mouse development","volume":"28","author":"Zhang","year":"2008","journal-title":"Mol. Cell. Biol."},{"key":"2021091611515014700_ref96","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/ncb1722","article-title":"The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1","volume":"10","author":"Gregory","year":"2008","journal-title":"Nat. Cell Biol."},{"key":"2021091611515014700_ref97","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1093\/ejo\/cjx034","article-title":"Differential microRNA expression in cultured palatal fibroblasts from infants with cleft palate and controls","volume":"40","author":"Schoen","year":"2017","journal-title":"Eur. J. Orthod."},{"key":"2021091611515014700_ref98","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1002\/ajmg.a.33236","article-title":"Single nucleotide polymorphism associated with nonsyndromic cleft palate influences the processing of miR-140","volume":"152","author":"Li","year":"2010","journal-title":"Am. J. Med. Genet. A"},{"key":"2021091611515014700_ref99","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1038\/ejhg.2012.55","article-title":"PDGFRa mutations in humans with isolated cleft palate","volume":"20","author":"Rattanasopha","year":"2012","journal-title":"Europ. J. Hum. Genet."},{"key":"2021091611515014700_ref100","doi-asserted-by":"crossref","first-page":"31054","DOI":"10.1038\/srep31054","article-title":"Associations between microRNA binding site SNPs in FGFs and FGFRs and the risk of non-syndromic orofacial cleft","volume":"6","author":"Li","year":"2016","journal-title":"Sci. Rep."},{"key":"2021091611515014700_ref101","doi-asserted-by":"crossref","first-page":"e1003785","DOI":"10.1371\/journal.pgen.1003785","article-title":"MicroRNA-17-92, a direct Ap-2\u03b1 transcriptional target, modulates T-box factor activity in orofacial clefting","volume":"9","author":"Wang","year":"2013","journal-title":"PLoS Genet."},{"key":"2021091611515014700_ref102","doi-asserted-by":"crossref","first-page":"86266","DOI":"10.18632\/oncotarget.13379","article-title":"Assessment of differentially expressed plasma microRNAs in nonsyndromic cleft palate and nonsyndromic cleft lip with cleft palate","volume":"7","author":"Li","year":"2016","journal-title":"Oncotarget"},{"key":"2021091611515014700_ref103","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1177\/0022034514527617","article-title":"A miRNA-binding-site SNP of MSX1 is associated with NSOC susceptibility","volume":"93","author":"Ma","year":"2014","journal-title":"J. Dent. Res."},{"key":"2021091611515014700_ref104","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1101\/gad.1915510","article-title":"MicroRNA-140 plays dual roles in both cartilage development and homeostasis","volume":"24","author":"Miyaki","year":"2010","journal-title":"Genes Dev."},{"key":"2021091611515014700_ref105","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1038\/gt.2016.22","article-title":"A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent","volume":"23","author":"Cao","year":"2016","journal-title":"Gene Ther."},{"key":"2021091611515014700_ref106","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1177\/0022034517716915","article-title":"Inhibition of the miR-17-92 cluster separates stages of palatogenesis","volume":"96","author":"Ries","year":"2017","journal-title":"J. Dent. Res."},{"key":"2021091611515014700_ref107","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/dgd.12140","article-title":"Micro RNA expression profiling of the developing murine upper lip","volume":"56","author":"Warner","year":"2014","journal-title":"Develop. Growth Differ."},{"key":"2021091611515014700_ref108","doi-asserted-by":"crossref","first-page":"D239","DOI":"10.1093\/nar\/gkv1258","article-title":"miRTarBase 2016: updates to the experimentally validated miRNA-target interactions database","volume":"44","author":"Chou","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"2021091611515014700_ref109","doi-asserted-by":"crossref","first-page":"e363","DOI":"10.1371\/journal.pbio.0020363","article-title":"Human microRNA targets","volume":"2","author":"John","year":"2004","journal-title":"PLoS Biol."},{"key":"2021091611515014700_ref110","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1038\/ng2135","article-title":"The role of site accessibility in microRNA target recognition","volume":"39","author":"Kertesz","year":"2007","journal-title":"Nat. Genet."},{"key":"2021091611515014700_ref111","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cell.2004.12.035","article-title":"Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets","volume":"120","author":"Lewis","year":"2005","journal-title":"Cell"},{"key":"2021091611515014700_ref112","doi-asserted-by":"crossref","first-page":"D762","DOI":"10.1093\/nar\/gkx1020","article-title":"The UCSC genome browser database: 2018 update","volume":"46","author":"Casper","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"2021091611515014700_ref113","doi-asserted-by":"crossref","first-page":"D108","DOI":"10.1093\/nar\/gkj143","article-title":"TRANSFAC\u00ae and its module TRANSCompel\u00ae: transcriptional gene regulation in eukaryotes","volume":"34","author":"Matys","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"2021091611515014700_ref114","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1038\/nmeth.2212","article-title":"A travel guide to Cytoscape plugins","volume":"9","author":"Saito","year":"2012","journal-title":"Nat. Methods"}],"container-title":["Human Molecular Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/hmg\/advance-article-pdf\/doi\/10.1093\/hmg\/ddab151\/38853261\/ddab151.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/hmg\/article-pdf\/30\/19\/1881\/40374155\/ddab151.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/hmg\/article-pdf\/30\/19\/1881\/40374155\/ddab151.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T11:54:42Z","timestamp":1631793282000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/hmg\/article\/30\/19\/1881\/6295110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,8]]},"references-count":114,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2021,6,8]]},"published-print":{"date-parts":[[2021,9,15]]}},"URL":"https:\/\/doi.org\/10.1093\/hmg\/ddab151","relation":{},"ISSN":["0964-6906","1460-2083"],"issn-type":[{"value":"0964-6906","type":"print"},{"value":"1460-2083","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,10,1]]},"published":{"date-parts":[[2021,6,8]]}}}