{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:09:02Z","timestamp":1790291342175,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U2004127"],"award-info":[{"award-number":["U2004127"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Activated neutrophil-derived exosomes reportedly contribute to the proliferation of airway smooth muscle cells (ASMCs), thereby aggravating the airway wall remodeling during asthma; however, the specific mechanism remains unclear. Lipopolysaccharide (LPS)-EXO and si-CRNDE-EXO were extracted from the media of human neutrophils treated with LPS and LPS\u2009+\u2009si-CRNDE (a siRNA targets long non-coding RNA CRNDE), respectively. Human ASMCs were co-cultured with LPS-EXO or si-CRNDE-EXO, and cell viability, proliferation and migration were measured. The interplay of colorectal neoplasia differentially expressed (CRNDE), inhibitor of nuclear factor kappa B kinase subunit beta (IKK\u03b2) and nuclear receptor subfamily 2 group C member 2 (TAK1) was explored using RNA immunoprecipitation (RIP) and Co-IP assays. A mouse model of asthma was induced using ovalbumin. CRNDE was upregulated in LPS-EXO and successfully transferred from LPS-treated neutrophils to ASMCs through exosome. Mechanically, CRNDE loaded in LPS-EXO reinforced TAK1-mediated IKK\u03b2 phosphorylation, thereby activating the nuclear factor kappa B (NF-\u03baB) pathway. Functionally, silencing CRNDE in LPS-EXO, an IKK\u03b2 inhibitor, and an NF-\u03baB inhibitor all removed the upregulation of cell viability, proliferation and migration induced by LPS-EXO in ASMCs. In the end, the in vivo experiment demonstrated that CRNDE knockdown in neutrophils effectively reduced the thickness of bronchial smooth muscle in a mouse model for asthma. Activated neutrophils-derived CRNDE was transferred to ASMCs through exosomes and activated the NF-\u03baB pathway by enhancing IKK\u03b2 phosphorylation. The latter promoted the proliferation and migration of ASMCs and then contributed to airway remodeling in asthma.<\/jats:p>","DOI":"10.1093\/hmg\/ddab283","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T11:15:40Z","timestamp":1633000540000},"page":"638-650","source":"Crossref","is-referenced-by-count":34,"title":["Exosomal transfer of activated neutrophil-derived lncRNA CRNDE promotes proliferation and migration of airway smooth muscle cells in asthma"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1201-4808","authenticated-orcid":false,"given":"Xiao-Yu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo-Chang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Hua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Li","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cui-Jie","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Jun","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Yi","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luo-Xian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease and Intensive Care, Henan Provincial People\u2019s Hospital, Zhengzhou 450003, People\u2019s Republic of China"},{"name":"Department of Respiratory Disease and Intensive Care, People\u2019s Hospital Affiliated to Zhengzhou University, Zhengzhou 450003, People\u2019s Republic of 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