{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T21:22:16Z","timestamp":1769721736110,"version":"3.49.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19AI1070235"],"award-info":[{"award-number":["U19AI1070235"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL132344"],"award-info":[{"award-number":["HL132344"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Skin deficiency of kinesin family member 3A causes disrupted skin barrier function and promotes development of atopic dermatitis (AD). It is not known how well Kif3aK14\u2206\/\u2206 mice approximate the human AD transcriptome. To determine the skin transcriptomic profile of Kif3aK14\u2206\/\u2206 mice and compare it with other murine AD models and human AD, we performed RNA-seq of full-thickness skin and epidermis from 3- and 8-week-old Kif3aK14\u2206\/\u2206 mice and compared the differentially expressed genes (DEGs) with transcriptomic datasets from mite-induced NC\/Nga, flaky tail (Tmem79ma\/ma Flgft\/ft), and filaggrin-mutant (Flgft\/ft) mice, as well as human AD transcriptome datasets including meta-analysis derived atopic dermatitis [MADAD] and the pediatric atopic dermatitis [PAD]. We then interrogated the Kif3aK14\u2206\/\u2206 skin DEGs using the LINCS-L1000 database to identify potential novel drug targets for AD treatment. We identified 471 and 901 DEGs at 3 and 8\u00a0weeks of age, respectively, in the absence of Kif3a. Kif3aK14\u2206\/\u2206 mice had 3.5\u20134.5 times more DEGs that overlapped with human AD DEGs compared to the flaky tail and Flgft\/ft mice. Further, 55%, 85% and 75% of 8-week Kif3aK14\u2206\/\u2206 DEGs overlapped with the MADAD and PAD non-lesional and lesional gene lists, respectively. Kif3aK14\u2206\/\u2206 mice spontaneously develop a human AD-like gene signature, which better represents pediatric non-lesional skin compared to other mouse models including flaky tail, Flgft\/ft and NC\/Nga. Thus, Kif3aK14\u2206\/\u2206 mice may model pediatric skin that is a precursor to the development of lesions and inflammation, and hence may be a useful model to study AD pathogenesis.<\/jats:p>","DOI":"10.1093\/hmg\/ddab342","type":"journal-article","created":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T20:11:57Z","timestamp":1637611917000},"page":"1588-1598","source":"Crossref","is-referenced-by-count":1,"title":["Skin depletion of <i>Kif3a<\/i> resembles the pediatric atopic dermatitis transcriptome profile"],"prefix":"10.1093","volume":"31","author":[{"given":"Mariana L","family":"Stevens","sequence":"first","affiliation":[{"name":"Division of Asthma Research, Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9189-8447","authenticated-orcid":false,"given":"Tesfaye B","family":"Mersha","sequence":"additional","affiliation":[{"name":"Division of Asthma Research, Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhonghua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Asthma Research, Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjun","family":"Kothari","sequence":"additional","affiliation":[{"name":"Division of Asthma Research, Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gurjit K","family":"Khurana Hershey","sequence":"additional","affiliation":[{"name":"Division of Asthma Research, Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,11,23]]},"reference":[{"key":"2022052023341595800_ref1","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1016\/S0140-6736(15)00149-X","article-title":"Atopic dermatitis","volume":"387","author":"Weidinger","year":"2016","journal-title":"Lancet"},{"key":"2022052023341595800_ref2","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.jaci.2007.05.042","article-title":"Risk of developing asthma in young children with atopic eczema: a systematic review","volume":"120","author":"Hulst","year":"2007","journal-title":"J. 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