{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T22:17:06Z","timestamp":1784067426182,"version":"3.55.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"21","license":[{"start":{"date-parts":[[2022,5,28]],"date-time":"2022-05-28T00:00:00Z","timestamp":1653696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000024","name":"CIHR","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Kabuki syndrome is frequently caused by loss-of-function mutations in one allele of histone 3 lysine 4 (H3K4) methyltransferase KMT2D and is associated with problems in neurological, immunological and skeletal system development. We generated heterozygous KMT2D knockout and Kabuki patient-derived cell models to investigate the role of reduced dosage of KMT2D in stem cells. We discovered chromosomal locus-specific alterations in gene expression, specifically a 110\u00a0Kb region containing Synaptotagmin 3 (SYT3), C-Type Lectin Domain Containing 11A (CLEC11A), Chromosome 19 Open Reading Frame 81 (C19ORF81) and SH3 And Multiple Ankyrin Repeat Domains 1 (SHANK1), suggesting locus-specific targeting of KMT2D. Using whole genome histone methylation mapping, we confirmed locus-specific changes in H3K4 methylation patterning coincident with regional decreases in gene expression in Kabuki cell models. Significantly reduced H3K4 peaks aligned with regions of stem cell maps of H3K27 and H3K4 methylation suggesting KMT2D haploinsufficiency impact bivalent enhancers in stem cells. Preparing the genome for subsequent differentiation cues may be of significant importance for Kabuki-related genes. This work provides a new insight into the mechanism of action of an important gene in bone and brain development and may increase our understanding of a specific function of a human disease-relevant H3K4 methyltransferase family member.<\/jats:p>","DOI":"10.1093\/hmg\/ddac121","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T20:33:55Z","timestamp":1654029235000},"page":"3715-3728","source":"Crossref","is-referenced-by-count":12,"title":["Kabuki syndrome stem cell models reveal locus specificity of histone methyltransferase 2D (KMT2D\/MLL4)"],"prefix":"10.1093","volume":"31","author":[{"given":"Malvin","family":"Jefri","sequence":"first","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick S","family":"Stumpf","sequence":"additional","affiliation":[{"name":"Institute for Computational Biomedicine , RWTH Aachen University, Aachen 52056 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"McKusick-Nathans Institute of Genetic Medicine , Johns Hopkins University School of Medicine, Baltimore, MD 21205 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huashan","family":"Peng","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuwan","family":"Hettige","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Francois","family":"Theroux","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahia","family":"Aouabed","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadija","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular , Washington University School of Medicine in St. Louis, St. Louis, MO 63110 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shriya","family":"Deshmukh","sequence":"additional","affiliation":[{"name":"Division of Experimental Medicine , Department of Medicine, McGill University, Montreal, QC H4A 3J1 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilit","family":"Antonyan","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjie","family":"Ni","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaima","family":"Alsuwaidi","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nada","family":"Jabado","sequence":"additional","affiliation":[{"name":"Division of Experimental Medicine , Department of Medicine, McGill University, Montreal, QC H4A 3J1 , Canada"},{"name":"Department of Human Genetics , McGill University, Montreal, QC H3A 0C7 , Canada"},{"name":"Department of Pediatrics , McGill University and The Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin A","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular , Washington University School of Medicine in St. Louis, St. Louis, MO 63110 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Schuppert","sequence":"additional","affiliation":[{"name":"Institute for Computational Biomedicine , RWTH Aachen University, Aachen 52056 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans T","family":"Bjornsson","sequence":"additional","affiliation":[{"name":"McKusick-Nathans Institute of Genetic Medicine , Johns Hopkins University School of Medicine, Baltimore, MD 21205 , USA"},{"name":"Faculty of Medicine , University of Iceland, Reykjavik, Iceland"},{"name":"Department of Genetics and Molecular Medicine , Landspitali University Hospital, 101 Reykjavik , Iceland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2959-221X","authenticated-orcid":false,"given":"Carl","family":"Ernst","sequence":"additional","affiliation":[{"name":"Psychiatric Genetics Group , McGill University, 6875 LaSalle Boulevard, Frank Common Building, Room 2101.2, Verdun, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Psychiatry , McGill University and Douglas Hospital Research Institute, Montreal, QC H4H 1R3 , Canada"},{"name":"Department of Human Genetics , McGill University, Montreal, QC H3A 0C7 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,5,28]]},"reference":[{"key":"2022102820035622600_ref1","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1002\/ajmg.1320310312","article-title":"Kabuki make-up (Niikawa-Kuroki) syndrome: a study of 62 patients","volume":"31","author":"Niikawa","year":"1988","journal-title":"Am. J. Med. Genet."},{"key":"2022102820035622600_ref2","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/ng.646","article-title":"Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome","volume":"42","author":"Ng","year":"2010","journal-title":"Nat. Genet."},{"key":"2022102820035622600_ref3","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.1002\/ajmg.a.36072","article-title":"MLL2 and KDM6A mutations in patients with Kabuki syndrome","volume":"161A","author":"Miyake","year":"2013","journal-title":"Am. J. Med. Genet. A"},{"key":"2022102820035622600_ref4","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.gene.2017.06.056","article-title":"Histone H3 lysine 4 methyltransferase KMT2D","volume":"627","author":"Froimchuk","year":"2017","journal-title":"Gene"},{"key":"2022102820035622600_ref5","doi-asserted-by":"crossref","first-page":"11871","DOI":"10.1073\/pnas.1606857113","article-title":"Enhancer priming by H3K4 methyltransferase MLL4 controls cell fate transition","volume":"113","author":"Wang","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2022102820035622600_ref6","doi-asserted-by":"crossref","first-page":"12902","DOI":"10.1073\/pnas.231473398","article-title":"COMPASS: a complex of proteins associated with a trithorax-related SET domain protein","volume":"98","author":"Miller","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2022102820035622600_ref7","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1146\/annurev-biochem-051710-134100","article-title":"The COMPASS family of histone H3K4 methylases: mechanisms of regulation in development and disease pathogenesis","volume":"81","author":"Shilatifard","year":"2012","journal-title":"Annu. Rev. Biochem."},{"key":"2022102820035622600_ref8","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1038\/nature16952","article-title":"Structural basis for activity regulation of MLL family methyltransferases","volume":"530","author":"Li","year":"2016","journal-title":"Nature"},{"key":"2022102820035622600_ref9","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/nrm3230","article-title":"Trithorax group proteins: switching genes on and keeping them active","volume":"12","author":"Schuettengruber","year":"2011","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2022102820035622600_ref10","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1038\/nrc3929","article-title":"Hijacked in cancer: the KMT2 (MLL) family of methyltransferases","volume":"15","author":"Rao","year":"2015","journal-title":"Nat. Rev. Cancer"},{"key":"2022102820035622600_ref11","doi-asserted-by":"crossref","first-page":"8513","DOI":"10.1073\/pnas.0902873106","article-title":"A tumor suppressive coactivator complex of p53 containing ASC-2 and histone H3-lysine-4 methyltransferase MLL3 or its paralogue MLL4","volume":"106","author":"Lee","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2022102820035622600_ref12","doi-asserted-by":"crossref","first-page":"a026427","DOI":"10.1101\/cshperspect.a026427","article-title":"MLL3\/MLL4\/COMPASS family on epigenetic regulation of enhancer function and cancer","volume":"6","author":"Sze","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"2022102820035622600_ref13","doi-asserted-by":"crossref","first-page":"e01503","DOI":"10.7554\/eLife.01503","article-title":"H3K4 mono- and di-methyltransferase MLL4 is required for enhancer activation during cell differentiation","volume":"2","author":"Lee","year":"2013","journal-title":"elife"},{"key":"2022102820035622600_ref14","doi-asserted-by":"crossref","first-page":"4745","DOI":"10.1128\/MCB.01181-13","article-title":"The MLL3\/MLL4 branches of the COMPASS family function as major histone H3K4 monomethylases at enhancers","volume":"33","author":"Hu","year":"2013","journal-title":"Mol. Cell. Biol."},{"key":"2022102820035622600_ref15","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.molcel.2007.06.022","article-title":"Activator-mediated recruitment of the MLL2 methyltransferase complex to the beta-globin locus","volume":"27","author":"Demers","year":"2007","journal-title":"Mol. Cell"},{"key":"2022102820035622600_ref16","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1038\/s41586-021-03903-7","article-title":"UTX condensation underlies its tumour-suppressive activity","volume":"597","author":"Shi","year":"2021","journal-title":"Nature"},{"key":"2022102820035622600_ref17","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1002\/sctm.16-0158","article-title":"A rapid pipeline to model rare neurodevelopmental disorders with simultaneous CRISPR\/Cas9 gene editing","volume":"6","author":"Bell","year":"2017","journal-title":"Stem Cells Transl. Med."},{"key":"2022102820035622600_ref18","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1038\/ng1403","article-title":"Nonsense-mediated decay approaches the clinic","volume":"36","author":"Holbrook","year":"2004","journal-title":"Nat. Genet."},{"key":"2022102820035622600_ref19","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1126\/science.1056794","article-title":"The human transcriptome map: clustering of highly expressed genes in chromosomal domains","volume":"291","author":"Caron","year":"2001","journal-title":"Science"},{"key":"2022102820035622600_ref20","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/nature09342","article-title":"Epigenetic memory in induced pluripotent stem cells","volume":"467","author":"Kim","year":"2010","journal-title":"Nature"},{"key":"2022102820035622600_ref21","doi-asserted-by":"crossref","first-page":"e129375","DOI":"10.1172\/jci.insight.129375","article-title":"Precocious neuronal differentiation and disrupted oxygen responses in Kabuki syndrome","volume":"4","author":"Carosso","year":"2019","journal-title":"JCI Insight"},{"key":"2022102820035622600_ref22","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/bs.mie.2015.12.007","article-title":"Identification and quantification of histone PTMs using high-resolution mass spectrometry","volume":"574","author":"Karch","year":"2016","journal-title":"Methods Enzymol."},{"key":"2022102820035622600_ref23","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.cell.2006.02.041","article-title":"A bivalent chromatin structure marks key developmental genes in embryonic stem cells","volume":"125","author":"Bernstein","year":"2006","journal-title":"Cell"},{"key":"2022102820035622600_ref24","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/j.molmed.2021.09.005","article-title":"Epigenetic priming in neurodevelopmental disorders","volume":"27","author":"Ernst","year":"2021","journal-title":"Trends Mol. Med."},{"key":"2022102820035622600_ref25","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1534\/genetics.118.301697","article-title":"Tissue-specific trans regulation of the mouse epigenome","volume":"211","author":"Baker","year":"2019","journal-title":"Genetics"},{"key":"2022102820035622600_ref26","doi-asserted-by":"crossref","first-page":"e1560","DOI":"10.1002\/wrna.1560","article-title":"How to get away with nonsense: mechanisms and consequences of escape from nonsense-mediated RNA decay","volume":"11","author":"Dyle","year":"2020","journal-title":"Wiley Interdiscip Rev RNA"},{"key":"2022102820035622600_ref27","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1038\/nrg3160","article-title":"Regulation of cytoplasmic mRNA decay","volume":"13","author":"Schoenberg","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"2022102820035622600_ref28","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1016\/j.molcel.2011.06.031","article-title":"RNA homeostasis governed by cell type-specific and branched feedback loops acting on NMD","volume":"43","author":"Huang","year":"2011","journal-title":"Mol. Cell"},{"key":"2022102820035622600_ref29","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1002\/humu.22547","article-title":"Molecular analysis, pathogenic mechanisms, and readthrough therapy on a large cohort of Kabuki syndrome patients","volume":"35","author":"Micale","year":"2014","journal-title":"Hum. Mutat."},{"key":"2022102820035622600_ref30","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1002\/ajmg.a.61518","article-title":"Phenotypic expansion of KMT2D-related disorder: beyond Kabuki syndrome","volume":"182","author":"Baldridge","year":"2020","journal-title":"Am. J. Med. Genet. A"},{"key":"2022102820035622600_ref31","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1002\/ajmg.a.61454","article-title":"Holoprosencephaly in Kabuki syndrome","volume":"182","author":"Daly","year":"2020","journal-title":"Am. J. Med. Genet. A"},{"key":"2022102820035622600_ref32","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/s10038-018-0536-6","article-title":"A comparative analysis of KMT2D missense variants in Kabuki syndrome, cancers and the general population","volume":"64","author":"Faundes","year":"2019","journal-title":"J. Hum. Genet."},{"key":"2022102820035622600_ref33","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1038\/s41436-019-0743-3","article-title":"A restricted spectrum of missense KMT2D variants cause a multiple malformations disorder distinct from Kabuki syndrome","volume":"22","author":"Cuvertino","year":"2020","journal-title":"Genet. Med."},{"key":"2022102820035622600_ref34","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/j.cell.2015.02.040","article-title":"The role of chromosome domains in shaping the functional genome","volume":"160","author":"Sexton","year":"2015","journal-title":"Cell"},{"key":"2022102820035622600_ref35","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1042\/BST20191164","article-title":"The MLL3\/4 H3K4 methyltransferase complex in establishing an active enhancer landscape","volume":"49","author":"Wang","year":"2021","journal-title":"Biochem. Soc. Trans."},{"key":"2022102820035622600_ref36","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1101\/gr.136184.111","article-title":"ChIP-seq guidelines and practices of the ENCODE and modENCODE consortia","volume":"22","author":"Landt","year":"2012","journal-title":"Genome Res."},{"key":"2022102820035622600_ref37","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.molcel.2017.06.028","article-title":"A UTX-MLL4-p300 transcriptional regulatory network coordinately shapes active enhancer landscapes for eliciting transcription","volume":"67","author":"Wang","year":"2017","journal-title":"Mol. Cell"},{"key":"2022102820035622600_ref38","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/j.ajhg.2012.03.017","article-title":"SHANK1 deletions in males with autism spectrum disorder","volume":"90","author":"Sato","year":"2012","journal-title":"Am. J. Hum. Genet."},{"key":"2022102820035622600_ref39","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.18782","article-title":"Clec11a\/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton","volume":"5","author":"Yue","year":"2016","journal-title":"Elife"},{"key":"2022102820035622600_ref40","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1038\/nprot.2012.016","article-title":"Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks","volume":"7","author":"Trapnell","year":"2012","journal-title":"Nat. Protoc."},{"key":"2022102820035622600_ref41","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","article-title":"Ultrafast and memory-efficient alignment of short DNA sequences to the human genome","volume":"10","author":"Langmead","year":"2009","journal-title":"Genome Biol."},{"key":"2022102820035622600_ref42","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s41587-020-0439-x","article-title":"The nf-core framework for community-curated bioinformatics pipelines","volume":"38","author":"Ewels","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"2022102820035622600_ref43","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The sequence alignment\/map format and SAMtools","volume":"25","author":"Li H","year":"2009","journal-title":"Bioinformatics"},{"key":"2022102820035622600_ref44","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1038\/nprot.2012.101","article-title":"Identifying ChIP-seq enrichment using MACS","volume":"7","author":"Feng","year":"2012","journal-title":"Nat. Protoc."},{"key":"2022102820035622600_ref45","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: an R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"OMICS"},{"key":"2022102820035622600_ref46","first-page":"54112","article-title":"Complete workflow for analysis of histone post-translational modifications using bottom-up mass spectrometry: from histone extraction to data analysis","volume":"111","author":"Sidoli","year":"2016","journal-title":"J. Vis. Exp."}],"container-title":["Human Molecular Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/hmg\/advance-article-pdf\/doi\/10.1093\/hmg\/ddac121\/44329436\/ddac121.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/hmg\/article-pdf\/31\/21\/3715\/46674183\/ddac121.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/hmg\/article-pdf\/31\/21\/3715\/46674183\/ddac121.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T20:04:52Z","timestamp":1666987492000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/hmg\/article\/31\/21\/3715\/6594512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,28]]},"references-count":46,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2022,5,28]]},"published-print":{"date-parts":[[2022,10,28]]}},"URL":"https:\/\/doi.org\/10.1093\/hmg\/ddac121","relation":{},"ISSN":["0964-6906","1460-2083"],"issn-type":[{"value":"0964-6906","type":"print"},{"value":"1460-2083","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,11,1]]},"published":{"date-parts":[[2022,5,28]]}}}