{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:21:34Z","timestamp":1787008894412,"version":"build-2736575974"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"8","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AG070681"],"award-info":[{"award-number":["R21AG070681"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AG075665"],"award-info":[{"award-number":["R21AG075665"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE030901"],"award-info":[{"award-number":["R01DE030901"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006034","name":"University of Southern California","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006034","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>G4C2 repeat expansion in C9orf72 causes the most common familial frontotemporal dementia and amyotrophic lateral sclerosis (C9FTD\/ALS). The pathogenesis includes haploinsufficiency of C9orf72, which forms a protein complex with Smcr8, as well as G4C2 repeat-induced gain of function including toxic dipeptide repeats (DPRs). The key in vivo disease-driving mechanisms and how loss- and gain-of-function interplay remain poorly understood. Here, we identified dysregulation of a lysosome-ribosome biogenesis circuit as an early and key disease mechanism using a physiologically relevant mouse model with combined loss- and gain-of-function across the aging process. C9orf72 deficiency exacerbates FTD\/ALS-like pathologies and behaviors in C9ORF72 bacterial artificial chromosome (C9-BAC) mice with G4C2 repeats under endogenous regulatory elements from patients. Single nucleus RNA sequencing (snRNA-seq) and bulk RNA-seq revealed that C9orf72 depletion disrupts lysosomes in neurons and leads to transcriptional dysregulation of ribosomal protein genes, which are likely due to the proteotoxic stress response and resemble ribosomopathy defects. Importantly, ectopic expression of C9orf72 or its partner Smcr8 in C9FTD\/ALS mutant mice promotes lysosomal functions and restores ribosome biogenesis gene transcription, resulting in the mitigation of DPR accumulation, neurodegeneration as well as FTD\/ALS-like motor and cognitive behaviors. Therefore, we conclude that loss- and gain-of-function crosstalk in C9FTD\/ALS converges on neuronal dysregulation of a lysosome-ribosome biogenesis circuit leading to proteotoxicity, neurodegeneration and behavioral defects.<\/jats:p>","DOI":"10.1093\/hmg\/ddac271","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T11:58:36Z","timestamp":1667390316000},"page":"1252-1265","source":"Crossref","is-referenced-by-count":4,"title":["Reversing lysosome-ribosome circuit dysregulation mitigates C9FTD\/ALS neurodegeneration and behaviors"],"prefix":"10.1093","volume":"32","author":[{"given":"Li","family":"Ma","sequence":"first","affiliation":[{"name":"University of Southern California (USC) Center for Craniofacial Molecular Biology, , Los Angeles, CA 90033 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Liang","sequence":"additional","affiliation":[{"name":"University of Southern California (USC) Center for Craniofacial Molecular Biology, , Los Angeles, CA 90033 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"University of California Los Angeles Department of Human Genetics, David Geffen School of Medicine, , Los Angeles, CA 90095 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Chang","sequence":"additional","affiliation":[{"name":"University of Southern California (USC) Center for Craniofacial Molecular Biology, , Los Angeles, CA 90033 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"University of Southern California (USC) Center for Craniofacial Molecular Biology, , Los Angeles, CA 90033 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Southern California (USC) Center for Craniofacial Molecular Biology, , Los Angeles, CA 90033 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanning","family":"Du","sequence":"additional","affiliation":[{"name":"University of Southern California (USC) Center for Craniofacial Molecular Biology, , Los Angeles, CA 90033 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jotham","family":"Sadan","sequence":"additional","affiliation":[{"name":"University of Southern California (USC) Center for Craniofacial Molecular Biology, , Los Angeles, CA 90033 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9474-5673","authenticated-orcid":false,"given":"Jian-Fu","family":"Chen","sequence":"additional","affiliation":[{"name":"University of Southern California (USC) Center for Craniofacial Molecular Biology, , Los Angeles, CA 90033 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"2023040610580493700_","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.neuron.2011.09.010","article-title":"A hexanucleotide repeat expansion in C9ORF72 is the cause of chromosome 9p21-linked ALS-FTD","volume":"72","author":"Renton","year":"2011","journal-title":"Neuron"},{"key":"2023040610580493700_","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.neuron.2011.09.011","article-title":"Expanded GGGGCC hexanucleotide repeat in noncoding region of C9ORF72 causes chromosome 9p-linked FTD and ALS","volume":"72","author":"DeJesus-Hernandez","year":"2011","journal-title":"Neuron"},{"key":"2023040610580493700_","doi-asserted-by":"crossref","first-page":"273","DOI":"10.2174\/156720511795563700","article-title":"FTD and ALS: a tale of two diseases","volume":"8","author":"Ferrari","year":"2011","journal-title":"Curr. 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