{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:32:45Z","timestamp":1790767965753,"version":"4.1.0"},"reference-count":65,"publisher":"Oxford University Press (OUP)","issue":"16","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>While Huntington\u2019s disease (HD) is widely recognized as a disease affecting the nervous system, much evidence has accumulated to suggest peripheral or non-neuronal tissues are affected as well. Here, we utilize the UAS\/GAL4 system to express a pathogenic HD construct in the muscle of the fly and characterize the effects. We observe detrimental phenotypes such as a reduced lifespan, decreased locomotion and accumulation of protein aggregates. Strikingly, depending on the GAL4 driver used to express the construct, we saw different aggregate distributions and severity of phenotypes. These different aggregate distributions were found to be dependent on the expression level and the timing of expression. Hsp70, a well-documented suppressor of polyglutamine aggregates, was found to strongly reduce the accumulation of aggregates in the eye, but in the muscle, it did not prevent the reduction of the lifespan. Therefore, the molecular mechanisms underlying the detrimental effects of aggregates in the muscle are distinct from the nervous system.<\/jats:p>","DOI":"10.1093\/hmg\/ddad098","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T00:08:35Z","timestamp":1687478915000},"page":"2656-2668","source":"Crossref","is-referenced-by-count":9,"title":["Juvenile and adult expression of polyglutamine expanded huntingtin produce distinct aggregate distributions in\n                    <i>Drosophila<\/i>\n                    muscle"],"prefix":"10.1093","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0301-1170","authenticated-orcid":false,"given":"Taylor","family":"Barwell","sequence":"first","affiliation":[{"name":"Department of Biology, Queen\u2019s University , 116 Barrie St, Kingston, Ontario, K7L 3N6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0098-222X","authenticated-orcid":false,"given":"Sehaj","family":"Raina","sequence":"additional","affiliation":[{"name":"Department of Biology, Queen\u2019s University , 116 Barrie St, Kingston, Ontario, K7L 3N6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin","family":"Page","sequence":"additional","affiliation":[{"name":"Department of Biology, Queen\u2019s University , 116 Barrie St, Kingston, Ontario, K7L 3N6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley","family":"MacCharles","sequence":"additional","affiliation":[{"name":"Department of Biology, Queen\u2019s University , 116 Barrie St, Kingston, Ontario, K7L 3N6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4785-3890","authenticated-orcid":false,"given":"Laurent","family":"Seroude","sequence":"additional","affiliation":[{"name":"Department of Biology, Queen\u2019s University , 116 Barrie St, Kingston, Ontario, K7L 3N6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,6,27]]},"reference":[{"key":"2023080808431487300_ref1","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1016\/j.neuron.2017.01.022","article-title":"Autophagy and neurodegeneration: pathogenic mechanisms and therapeutic opportunities","volume":"93","author":"Menzies","year":"2017","journal-title":"Neuron"},{"key":"2023080808431487300_ref2","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.neuron.2016.02.003","article-title":"The biology of huntingtin","volume":"89","author":"Saudou","year":"2016","journal-title":"Neuron"},{"key":"2023080808431487300_ref3","doi-asserted-by":"crossref","first-page":"5659","DOI":"10.1038\/ncomms6659","article-title":"The role of protein clearance mechanisms in organismal ageing and age-related diseases","volume":"5","author":"Vilchez","year":"2014","journal-title":"Nat. Commun."},{"key":"2023080808431487300_ref4","volume-title":"Molecular Genetics of Axial Patterning, Growth and Disease in Drosophila Eye","author":"Fernandez-Funez","year":"2020"},{"key":"2023080808431487300_ref5","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/978-981-13-2218-1_3","volume-title":"Insights into Human Neurodegeneration: Lessons Learnt from Drosophila","author":"Mandya","year":"2019"},{"key":"2023080808431487300_ref6","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1017\/CBO9780511920585.012","volume-title":"Behavioral Genetics of the Fly (Drosophila melanogaster)","author":"Roy","year":"2014"},{"key":"2023080808431487300_ref7","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/S0896-6273(00)80573-5","article-title":"Polyglutamine-expanded human huntingtin transgenes induce degeneration of drosophila photoreceptor neurons","volume":"21","author":"Jackson","year":"1998","journal-title":"Neuron"},{"key":"2023080808431487300_ref8","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/35099568","article-title":"Histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in drosophila","volume":"413","author":"Steffan","year":"2001","journal-title":"Nature"},{"key":"2023080808431487300_ref9","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/S0092-8674(00)81200-3","article-title":"Expanded polyglutamine protein forms nuclear inclusions and causes neural degeneration in drosophila","volume":"93","author":"Warrick","year":"1998","journal-title":"Cell"},{"key":"2023080808431487300_ref10","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/hmg\/9.1.13","article-title":"Expanded polyglutamine peptides alone are intrinsically cytotoxic and cause neurodegeneration in drosophila","volume":"9","author":"Marsh","year":"2000","journal-title":"Hum. Mol. Genet."},{"key":"2023080808431487300_ref11","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1126\/science.287.5459.1837","article-title":"Genetic suppression of polyglutamine toxicity in drosophila","volume":"287","author":"Kazemi-Esfarjani","year":"2000","journal-title":"Science"},{"key":"2023080808431487300_ref12","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/35040584","article-title":"Identification of genes that modify ataxin-1-induced neurodegeneration","volume":"408","author":"Fernandez-Funez","year":"2000","journal-title":"Nature"},{"key":"2023080808431487300_ref13","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/0896-6273(93)90127-D","article-title":"Huntington's disease gene (IT15) is widely expressed in human and rat tissues","volume":"11","author":"Li","year":"1993","journal-title":"Neuron"},{"key":"2023080808431487300_ref14","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/ng1193-259","article-title":"Widespread expression of the human and rat Huntington's disease gene in brain and nonneural tissues","volume":"5","author":"Strong","year":"1993","journal-title":"Nat. Genet."},{"key":"2023080808431487300_ref15","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/0896-6273(95)90345-3","article-title":"Widespread expression of Huntington's disease gene (IT15) protein product","volume":"14","author":"Sharp","year":"1995","journal-title":"Neuron"},{"key":"2023080808431487300_ref16","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/ng0595-104","article-title":"Cellular localization of the Huntington's disease protein and discrimination of the normal and mutated form","volume":"10","author":"Trottier","year":"1995","journal-title":"Nat. Genet."},{"key":"2023080808431487300_ref17","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1093\/hmg\/8.5.813","article-title":"Formation of polyglutamine inclusions in non-CNS tissue","volume":"8","author":"Sathasivam","year":"1999","journal-title":"Hum. Mol. Genet."},{"key":"2023080808431487300_ref18","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1002\/mds.20373","article-title":"Mitochondrial impairment in patients and asymptomatic mutation carriers of Huntington's disease","volume":"20","author":"Saft","year":"2005","journal-title":"Mov. Disord."},{"key":"2023080808431487300_ref19","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1002\/mds.21540","article-title":"Clinical correlates of mitochondrial function in Huntington's disease muscle","volume":"22","author":"Turner","year":"2007","journal-title":"Mov. Disord."},{"key":"2023080808431487300_ref20","doi-asserted-by":"crossref","first-page":"e8025","DOI":"10.1371\/journal.pone.0008025","article-title":"Formation of polyglutamine inclusions in a wide range of non-CNS tissues in the HdhQ150 knock-in mouse model of Huntington's disease","volume":"4","author":"Moffitt","year":"2009","journal-title":"PLoS One"},{"key":"2023080808431487300_ref21","doi-asserted-by":"crossref","first-page":"e0173644","DOI":"10.1371\/journal.pone.0173644","article-title":"A new Caenorhabditis elegans model of human huntingtin 513 aggregation and toxicity in body wall muscles","volume":"12","author":"Lee","year":"2017","journal-title":"PLoS One"},{"key":"2023080808431487300_ref22","doi-asserted-by":"crossref","first-page":"14275","DOI":"10.1038\/s41598-017-14290-3","article-title":"Myostatin inhibition prevents skeletal muscle pathophysiology in Huntington's disease mice","volume":"7","author":"Bondulich","year":"2017","journal-title":"Sci. Rep."},{"key":"2023080808431487300_ref23","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1093\/hmg\/ddy077","article-title":"A genetic modifier suggests that endurance exercise exacerbates Huntington's disease","volume":"27","author":"Corrochano","year":"2018","journal-title":"Hum. Mol. Genet."},{"key":"2023080808431487300_ref24","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1002\/mds.21550","article-title":"Myopathy as a first symptom of Huntington's disease in a Marathon runner","volume":"22","author":"Kosinski","year":"2007","journal-title":"Mov. Disord."},{"key":"2023080808431487300_ref25","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1242\/dev.118.2.401","article-title":"Targeted gene expression as a means of altering cell fates and generating dominant phenotypes","volume":"118","author":"Brand","year":"1993","journal-title":"Development"},{"key":"2023080808431487300_ref26","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/j.freeradbiomed.2009.06.021","article-title":"Sod2 knockdown in the musculature has whole-organism consequences in drosophila","volume":"47","author":"Martin","year":"2009","journal-title":"Free Radic. Biol. Med."},{"key":"2023080808431487300_ref27","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1111\/j.1474-9726.2008.00421.x","article-title":"Characterization of the drosophila gene-switch system in aging studies: a cautionary tale","volume":"7","author":"Poirier","year":"2008","journal-title":"Aging Cell"},{"key":"2023080808431487300_ref28","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1046\/j.1474-9728.2002.00007.x","article-title":"Spatio-temporal analysis of gene expression during aging in Drosophila melanogaster","volume":"1","author":"Seroude","year":"2002","journal-title":"Aging Cell"},{"key":"2023080808431487300_ref29","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1534\/genetics.109.112516","article-title":"A genomewide RNA interference screen for modifiers of aggregates formation by mutant huntingtin in drosophila","volume":"184","author":"Zhang","year":"2010","journal-title":"Genetics"},{"key":"2023080808431487300_ref30","doi-asserted-by":"crossref","first-page":"12596","DOI":"10.1073\/pnas.221303298","article-title":"A conditional tissue-specific transgene expression system using inducible GAL4","volume":"98","author":"Osterwalder","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2023080808431487300_ref31","doi-asserted-by":"crossref","first-page":"3037","DOI":"10.1242\/dev.125.16.3037","article-title":"Divergent roles for NK-2 class homeobox genes in cardiogenesis in flies and mice","volume":"125","author":"Ranganayakulu","year":"1998","journal-title":"Development"},{"key":"2023080808431487300_ref32","doi-asserted-by":"crossref","first-page":"e4167","DOI":"10.7717\/peerj.4167","article-title":"Regulating the UAS\/GAL4 system in adult drosophila with Tet-off GAL80 transgenes","volume":"5","author":"Barwell","year":"2017","journal-title":"PeerJ"},{"key":"2023080808431487300_ref33","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.63726","article-title":"The hippo pathway controls myofibril assembly and muscle fiber growth by regulating sarcomeric gene expression","volume":"10","author":"Kaya-Copur","year":"2021","journal-title":"elife"},{"key":"2023080808431487300_ref34","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.79903","article-title":"Fixation can change the appearance of phase separation in living cells","volume":"11","author":"Irgen-Gioro","year":"2022","journal-title":"elife"},{"key":"2023080808431487300_ref35","doi-asserted-by":"crossref","first-page":"12602","DOI":"10.1073\/pnas.221303998","article-title":"P[switch], a system for spatial and temporal control of gene expression in Drosophila melanogaster","volume":"98","author":"Roman","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2023080808431487300_ref36","doi-asserted-by":"crossref","first-page":"2839","DOI":"10.1534\/g3.116.030882","article-title":"Ligand-bound GeneSwitch causes developmental aberrations in drosophila that are alleviated by the alternative oxidase","volume":"6","author":"Andjelkovic","year":"2016","journal-title":"G3 (Bethesda)"},{"key":"2023080808431487300_ref37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/gene.10150","article-title":"GAL4 system in drosophila: a fly geneticist's Swiss army knife","volume":"34","author":"Duffy","year":"2002","journal-title":"Genesis"},{"key":"2023080808431487300_ref38","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1126\/science.1089035","article-title":"Spatiotemporal rescue of memory dysfunction in drosophila","volume":"302","author":"McGuire","year":"2003","journal-title":"Science"},{"key":"2023080808431487300_ref39","article-title":"Comparison of GAL80ts and Tet-off GAL80 transgenes","volume":"2023","author":"Barwell","year":"2023","journal-title":"MicroPubl Biol."},{"key":"2023080808431487300_ref40","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1038\/220808a0","article-title":"Temperature and lifespan in drosophila","volume":"220","author":"Lamb","year":"1968","journal-title":"Nature"},{"key":"2023080808431487300_ref41","doi-asserted-by":"crossref","first-page":"9737","DOI":"10.1073\/pnas.1618994114","article-title":"The 4E-BP growth pathway regulates the effect of ambient temperature on drosophila metabolism and lifespan","volume":"114","author":"Carvalho","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2023080808431487300_ref42","doi-asserted-by":"crossref","first-page":"12974","DOI":"10.1073\/pnas.0405207101","article-title":"Drosophila lifespan enhancement by exogenous bacteria","volume":"101","author":"Brummel","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2023080808431487300_ref43","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/70532","article-title":"Suppression of polyglutamine-mediated neurodegeneration in drosophila by the molecular chaperone HSP70","volume":"23","author":"Warrick","year":"1999","journal-title":"Nat. Genet."},{"key":"2023080808431487300_ref44","doi-asserted-by":"crossref","first-page":"4197","DOI":"10.1534\/g3.119.400782","article-title":"Expression of heat shock protein 70 is insufficient to extend Drosophila melanogaster longevity","volume":"9","author":"Xiao","year":"2019","journal-title":"G3 (Bethesda)"},{"key":"2023080808431487300_ref45","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1002\/dneu.20344","article-title":"Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in drosophila","volume":"67","author":"Xiao","year":"2007","journal-title":"Dev. Neurobiol."},{"key":"2023080808431487300_ref46","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1126\/science.aax3338","article-title":"Huntington's disease alters human neurodevelopment","volume":"369","author":"Barnat","year":"2020","journal-title":"Science"},{"key":"2023080808431487300_ref47","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ymeth.2014.02.037","article-title":"A guide to study drosophila muscle biology","volume":"68","author":"Weitkunat","year":"2014","journal-title":"Methods"},{"key":"2023080808431487300_ref48","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.21769\/BioProtoc.2606","article-title":"Rapid IFM dissection for visualizing fluorescently tagged Sarcomeric proteins","volume":"7","author":"Xiao","year":"2017","journal-title":"Bio Protoc"},{"key":"2023080808431487300_ref49","doi-asserted-by":"crossref","first-page":"101015","DOI":"10.1016\/j.disamonth.2020.101015","article-title":"Rhabdomyolysis","volume":"66","author":"Cabral","year":"2020","journal-title":"Dis. Mon."},{"key":"2023080808431487300_ref50","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1093\/hmg\/11.17.1911","article-title":"Dysregulation of gene expression in the R6\/2 model of polyglutamine disease: parallel changes in muscle and brain","volume":"11","author":"Luthi-Carter","year":"2002","journal-title":"Hum. Mol. Genet."},{"key":"2023080808431487300_ref51","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1159\/000321681","article-title":"Impaired brain creatine kinase activity in Huntington's disease","volume":"8","author":"Zhang","year":"2011","journal-title":"Neurodegener. Dis."},{"key":"2023080808431487300_ref52","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1006\/nbdi.2001.0406","article-title":"Creatine increase survival and delays motor symptoms in a transgenic animal model of Huntington's disease","volume":"8","author":"Andreassen","year":"2001","journal-title":"Neurobiol. Dis."},{"key":"2023080808431487300_ref53","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1046\/j.1471-4159.2003.01706.x","article-title":"Creatine therapy provides neuroprotection after onset of clinical symptoms in Huntington's disease transgenic mice","volume":"85","author":"Dedeoglu","year":"2003","journal-title":"J. Neurochem."},{"key":"2023080808431487300_ref54","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jinsphys.2014.02.005","article-title":"A high-throughput method of hemolymph extraction from adult drosophila without anesthesia","volume":"63","author":"MacMillan","year":"2014","journal-title":"J. Insect Physiol."},{"key":"2023080808431487300_ref55","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1242\/dev.113.1.67","article-title":"Development of the indirect flight muscles of drosophila","volume":"113","author":"Fernandes","year":"1991","journal-title":"Development"},{"key":"2023080808431487300_ref56","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.248336","article-title":"An autophagy-dependent tubular lysosomal network synchronizes degradative activity required for muscle remodeling","volume":"133","author":"Murakawa","year":"2020","journal-title":"J. Cell Sci."},{"key":"2023080808431487300_ref57","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1038\/s41467-021-22058-7","article-title":"Myofibril and mitochondria morphogenesis are coordinated by a mechanical feedback mechanism in muscle","volume":"12","author":"Avellaneda","year":"2021","journal-title":"Nat. Commun."},{"key":"2023080808431487300_ref58","doi-asserted-by":"crossref","first-page":"203760","DOI":"10.1016\/j.cdev.2021.203760","article-title":"Mechanobiology of muscle and myofibril morphogenesis","volume":"168","author":"Luis","year":"2021","journal-title":"Cells Dev"},{"key":"2023080808431487300_ref59","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.mad.2005.09.012","article-title":"Juvenile Huntington's disease: does a dosage-effect pathogenic mechanism differ from the classical adult disease?","volume":"127","author":"Squitieri","year":"2006","journal-title":"Mech. Ageing Dev."},{"key":"2023080808431487300_ref60","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/S0092-8674(00)81385-9","article-title":"Reiterative use of the EGF receptor triggers differentiation of all cell types in the drosophila eye","volume":"87","author":"Freeman","year":"1996","journal-title":"Cell"},{"key":"2023080808431487300_ref61","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1007\/978-3-319-10605-2_50","article-title":"Detecting social actions of fruit flies","volume":"8690","author":"Eyjolfsdottir","year":"2014","journal-title":"Lect Notes Comput Sc"},{"key":"2023080808431487300_ref62","doi-asserted-by":"crossref","first-page":"101888","DOI":"10.1016\/j.xpro.2022.101888","article-title":"Protocol for recording and analyzing spontaneous locomotion in drosophila","volume":"3","author":"Barwell","year":"2022","journal-title":"STAR Protocols"},{"key":"2023080808431487300_ref63","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1139\/gen-2020-0044","article-title":"Versatile method to measure locomotion in adult drosophila","volume":"64","author":"Barwell","year":"2021","journal-title":"Genome"},{"key":"2023080808431487300_ref64","doi-asserted-by":"crossref","first-page":"101843","DOI":"10.1016\/j.xpro.2022.101843","article-title":"Protocol to measure \u00df-galactosidase in drosophila extracts using a CPRG assay","volume":"3","author":"Barwell","year":"2022","journal-title":"STAR Protocols"},{"key":"2023080808431487300_ref65","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1515\/znc-1979-1-228","article-title":"Isolation of anatomical brain mutants of drosophila by histological means","volume":"34","author":"Heisenberg","year":"1979","journal-title":"Z. Naturforsch. C"}],"container-title":["Human Molecular Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/hmg\/advance-article-pdf\/doi\/10.1093\/hmg\/ddad098\/50884883\/ddad098.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/hmg\/article-pdf\/32\/16\/2656\/51047862\/ddad098.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/hmg\/article-pdf\/32\/16\/2656\/51047862\/ddad098.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T16:47:50Z","timestamp":1729615670000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/hmg\/article\/32\/16\/2656\/7208880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,27]]},"references-count":65,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2023,6,27]]},"published-print":{"date-parts":[[2023,8,7]]}},"URL":"https:\/\/doi.org\/10.1093\/hmg\/ddad098","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.04.04.535619","asserted-by":"object"}]},"ISSN":["0964-6906","1460-2083"],"issn-type":[{"value":"0964-6906","type":"print"},{"value":"1460-2083","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,8,15]]},"published":{"date-parts":[[2023,6,27]]}}}