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This observation supports the concept that monogenic neurodevelopmental disorders are systemic diseases that profoundly impact neurodevelopment. We tested the systemic disease model focusing on Rett syndrome, which is caused by mutations in MECP2. Transcriptomes and proteomes of organs and brain regions from Mecp2-null mice as well as diverse MECP2-null male and female human cells were assessed. Widespread changes in the steady-state transcriptome and proteome were identified in brain regions and organs of presymptomatic Mecp2-null male mice as well as mutant human cell lines. The extent of these transcriptome and proteome modifications was similar in cortex, liver, kidney, and skeletal muscle and more pronounced than in the hippocampus and striatum. In particular, Mecp2- and MECP2-sensitive proteomes were enriched in synaptic and metabolic annotated gene products, the latter encompassing lipid metabolism and mitochondrial pathways. MECP2 mutations altered pyruvate-dependent mitochondrial respiration while maintaining the capacity to use glutamine as a mitochondrial carbon source. We conclude that mutations in Mecp2\/MECP2 perturb lipid and mitochondrial metabolism systemically limiting cellular flexibility to utilize mitochondrial fuels.<\/jats:p>","DOI":"10.1093\/hmg\/ddad154","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T01:13:28Z","timestamp":1694567608000},"page":"12-32","source":"Crossref","is-referenced-by-count":14,"title":["Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants"],"prefix":"10.1093","volume":"33","author":[{"given":"Stephanie A","family":"Zlatic","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Emory University , 615 Michael Steet, Atlanta, GA 30322 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Emory University , 615 Michael Steet, Atlanta, GA 30322 ,","place":["United 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Upstate Medical University , 505 Irving Avenue, Syracuse, NY 13210 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph Martin","family":"Dalloul","sequence":"additional","affiliation":[{"name":"Pharmacology and Chemical Biology, Emory University , 1510 Clifton Rd NE, Atlanta, GA 30322 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William Li-Yun","family":"Liu","sequence":"additional","affiliation":[{"name":"Pharmacology and Chemical Biology, Emory University , 1510 Clifton Rd NE, Atlanta, GA 30322 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anupam","family":"Patgiri","sequence":"additional","affiliation":[{"name":"Pharmacology and Chemical Biology, Emory University , 1510 Clifton Rd NE, Atlanta, GA 30322 ,","place":["United 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