{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T10:39:27Z","timestamp":1777286367442,"version":"3.51.4"},"reference-count":96,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T00:00:00Z","timestamp":1695340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"CIHR and Genome Canada","award":["CEE-151619"],"award-info":[{"award-number":["CEE-151619"]}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["FND-148425"],"award-info":[{"award-number":["FND-148425"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Assisted reproductive technologies (ART) account for 1\u20136% of births in developed countries. While most children conceived are healthy, increases in birth and genomic imprinting defects have been reported; such abnormal outcomes have been attributed to underlying parental infertility and\/or the ART used. Here, we assessed whether paternal genetic and lifestyle factors, that are associated with male infertility and affect the sperm epigenome, can influence ART outcomes. We examined how paternal factors, haploinsufficiency for Dnmt3L, an important co-factor for DNA methylation reactions, and\/or diet-induced obesity, in combination with ART (superovulation, in vitro fertilization, embryo culture and embryo transfer), could adversely influence embryo development and DNA methylation patterning in mice. While male mice fed high-fat diets (HFD) gained weight and showed perturbed metabolic health, their sperm DNA methylation was minimally affected by the diet. In contrast, Dnmt3L haploinsufficiency induced a marked loss of DNA methylation in sperm; notably, regions affected were associated with neurodevelopmental pathways and enriched in young retrotransposons, sequences that can have functional consequences in the next generation. Following ART, placental imprinted gene methylation and growth parameters were impacted by one or both paternal factors. For embryos conceived by natural conception, abnormality rates were similar for WT and Dnmt3L+\/\u2212 fathers. In contrast, paternal Dnmt3L+\/\u2212 genotype, as compared to WT fathers, resulted in a 3-fold increase in the incidence of morphological abnormalities in embryos generated by ART. Together, the results indicate that embryonic morphological and epigenetic defects associated with ART may be exacerbated in offspring conceived by fathers with sperm epimutations.<\/jats:p>","DOI":"10.1093\/hmg\/ddad160","type":"journal-article","created":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T04:16:11Z","timestamp":1695442571000},"page":"48-63","source":"Crossref","is-referenced-by-count":7,"title":["Higher incidence of embryonic defects in mouse offspring conceived with assisted reproduction from fathers with sperm epimutations"],"prefix":"10.1093","volume":"33","author":[{"given":"Gurbet","family":"Karahan","sequence":"first","affiliation":[{"name":"McGill University Department of Human Genetics, , Montreal, QC, H3A 0C7 , Canada"},{"name":"Research Institute of the McGill University Health Centre , Montreal, QC, H4A 3J1 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9e","family":"Martel","sequence":"additional","affiliation":[{"name":"Research Institute of the McGill University Health Centre , Montreal, QC, H4A 3J1 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"Rahimi","sequence":"additional","affiliation":[{"name":"Research Institute of the McGill University Health Centre , Montreal, QC, H4A 3J1 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mena","family":"Farag","sequence":"additional","affiliation":[{"name":"McGill University Department of Human Genetics, , Montreal, QC, H3A 0C7 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Matias","sequence":"additional","affiliation":[{"name":"Health Canada Nutrition Research Division, , Ottawa, ON, K1A 0K9 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda J","family":"MacFarlane","sequence":"additional","affiliation":[{"name":"Health Canada Nutrition Research Division, , Ottawa, ON, K1A 0K9 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donovan","family":"Chan","sequence":"additional","affiliation":[{"name":"Research Institute of the McGill University Health Centre , Montreal, QC, H4A 3J1 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacquetta","family":"Trasler","sequence":"additional","affiliation":[{"name":"McGill University Department of Human Genetics, , Montreal, QC, H3A 0C7 , Canada"},{"name":"Research Institute of the McGill University Health Centre , Montreal, QC, H4A 3J1 , Canada"},{"name":"McGill University Department of Pharmacology and Therapeutics, , Montreal, QC, H3G 1Y6 , Canada"},{"name":"McGill University Health Centre Department of Pediatrics, , Montreal, QC, H4A 3J1 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,9,22]]},"reference":[{"key":"2023121917045878900_ref1","doi-asserted-by":"crossref","first-page":"hoac022","DOI":"10.1093\/hropen\/hoac022","article-title":"ART in Europe, 2018: results generated from European registries by ESHRE","volume":"2022","author":"European IVF Monitoring Consortium (EIM)","year":"2022","journal-title":"Hum Reprod Open"},{"key":"2023121917045878900_ref2","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1080\/14767058.2018.1488168","article-title":"Do the children born after assisted reproductive technology have an increased risk of birth defects? A systematic review and meta-analysis","volume":"33","author":"Zhao","year":"2020","journal-title":"J Matern Fetal Neonatal Med"},{"key":"2023121917045878900_ref3","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1086\/346031","article-title":"Association of in vitro fertilization with Beckwith-Wiedemann syndrome and epigenetic alterations of LIT1 and H19","volume":"72","author":"DeBaun","year":"2003","journal-title":"Am J Hum Genet"},{"key":"2023121917045878900_ref4","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1136\/jmg.2004.026930","article-title":"Increased prevalence of imprinting defects in patients with Angelman syndrome born to subfertile couples","volume":"42","author":"Ludwig","year":"2005","journal-title":"J Med Genet"},{"key":"2023121917045878900_ref5","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1093\/humrep\/des197","article-title":"Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted 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