{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:47:08Z","timestamp":1789984028117,"version":"4.0.1"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T00:00:00Z","timestamp":1705276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["81270766"],"award-info":[{"award-number":["81270766"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["82170718"],"award-info":[{"award-number":["82170718"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovation of Science and Technology, Fujian province","award":["2020Y9158"],"award-info":[{"award-number":["2020Y9158"]}]},{"name":"Natural Science Foundation of Fujian Province of China","award":["2021J01411"],"award-info":[{"award-number":["2021J01411"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>More than 60 monogenic genes mutated in steroid-resistant nephrotic syndrome (SRNS) have been identified. Our previous study found that mutations in nucleoporin 160 kD (NUP160) are implicated in SRNS. The NUP160 gene encodes a component of the nuclear pore complex. Recently, two siblings with homozygous NUP160 mutations presented with SRNS and a nervous system disorder. However, replication of nephrotic syndrome (NS)-associated phenotypes in a mammalian model following loss of Nup160 is needed to prove that NUP160 mutations cause SRNS. Here, we generated a podocyte-specific Nup160 knockout (Nup160podKO) mouse model using CRISPR\/Cas9 and Cre\/loxP technologies. We investigated NS-associated phenotypes in these Nup160podKO mice. We verified efficient abrogation of Nup160 in Nup160podKO mice at both the DNA and protein levels. We showed that Nup160podKO mice develop typical signs of NS. Nup160podKO mice exhibited progression of proteinuria to average albumin\/creatinine ratio (ACR) levels of 15.06\u2009\u00b1\u20092.71\u00a0mg\/mg at 26\u00a0weeks, and had lower serum albumin levels of 13.13\u2009\u00b1\u20091.34\u00a0g\/l at 30\u00a0weeks. Littermate control mice had urinary ACR mean values of 0.03\u00a0mg\/mg and serum albumin values of 22.89\u2009\u00b1\u20090.34\u00a0g\/l at the corresponding ages. Further, Nup160podKO mice exhibited glomerulosclerosis compared with littermate control mice. Podocyte-specific Nup160 knockout in mice led to NS and glomerulosclerosis. Thus, our findings strongly support that mutations in NUP160 cause SRNS. The newly generated Nup160podKO mice are a reliable mammalian model for future study of the pathogenesis of NUP160-associated SRNS.<\/jats:p>","DOI":"10.1093\/hmg\/ddad211","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T23:29:01Z","timestamp":1705361341000},"page":"667-676","source":"Crossref","is-referenced-by-count":10,"title":["Podocyte-specific <i>Nup160<\/i> knockout mice develop nephrotic syndrome and glomerulosclerosis"],"prefix":"10.1093","volume":"33","author":[{"given":"Yuanyuan","family":"Li","sequence":"first","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 , China"},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children's Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 , China"},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 , China"},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children's Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 , China"},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 , China"},{"name":"Department of Pediatrics , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"},{"name":"Fujian Medical University , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children's Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"},{"name":"Fujian Medical University , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojian","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children's Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"},{"name":"Fujian Medical University , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children's Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shentong","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathology , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"},{"name":"Fujian Medical University , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huajuan","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children's Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lifang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"},{"name":"Fujian Medical University , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children's Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Pathology , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"},{"name":"Fujian Medical University , Fuzong Clinical Medical College, , 156 Xi Er Huan Bei Road, Fuzhou, Fujian 350025 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihua","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 , China"},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children's Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 , China"},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 , 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