{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T00:45:15Z","timestamp":1792889115528,"version":"4.5.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2024J032"],"award-info":[{"award-number":["2024J032"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2023J032"],"award-info":[{"award-number":["2023J032"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Medical and Health Plan of Zhejiang","award":["2025KY1286"],"award-info":[{"award-number":["2025KY1286"]}]},{"name":"Medical and Health Plan of Zhejiang","award":["2023KY1025"],"award-info":[{"award-number":["2023KY1025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study investigates the influence of miR-128-2-5p within serum-derived exosomes (Exos) on COL6A2 expression and its implications in postmenopausal osteoporosis (POMP). Utilizing bioinformatics analysis, we identified 1317 differentially expressed genes (DEGs), primarily enriched in the focal adhesion pathway\u2014a critical regulator of osteoblast adhesion. A significant gene, COL6A2, emerged as notably downregulated in POMP, possessing potential as a diagnostic marker. Predictive analysis linked the upstream miRNA miR-128-2-5p, highly enriched in Exos, with the regulation of COL6A2. Experimentally, Exos from POMP patients demonstrated elevated miR-128-2-5p levels, which inhibited COL6A2 expression in vitro, reducing osteoblast adhesion and exacerbating osteoporotic conditions. These findings highlight the pivotal role of exosomal miR-128-2-5p in bone metabolism, suggesting a novel molecular mechanism and a potential therapeutic target in POMP.<\/jats:p>","DOI":"10.1093\/hmg\/ddae147","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T11:53:46Z","timestamp":1737028426000},"page":"563-576","source":"Crossref","is-referenced-by-count":3,"title":["Regulatory role of miR-128-2-5p in serum exosomes on COL6A2 expression and postmenopausal osteoporosis"],"prefix":"10.1093","volume":"34","author":[{"given":"Liangjie","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Orthopedics , Ningbo Medical Center Li Huili Hospital, , No. 57 Xingning Road, Yinzhou District, Ningbo, Zhejiang 315040,","place":["China"]},{"name":"Li Huili Hospital Affiliated to Ningbo University , Ningbo Medical Center Li Huili Hospital, , No. 57 Xingning Road, Yinzhou District, Ningbo, Zhejiang 315040,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The First Hospital of Jilin University , No. 71, Xinmin Street, Chaoyang District, Changchun City, Jilin Province 130021,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Zhengzhou Central Hospital Affiliated to Zhengzhou University , No. 195, Tongbai Road, Zhongyuan District, Zhengzhou City, South Province 450007,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6404-8625","authenticated-orcid":false,"given":"Minjie","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Jiujiang No.1 People\u2019s Hospital , No. 48, Taling South Road, Xunyang District, Jiujiang City, Jiangxi Province 332000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"2025032012274734000_ref1","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1097\/PPO.0000000000000592","article-title":"Menopause hormone therapy in the Management of Postmenopausal Osteoporosis","volume":"28","author":"Rozenberg","year":"2022","journal-title":"Cancer J"},{"key":"2025032012274734000_ref2","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.rbr.2017.06.001","article-title":"Brazilian guidelines for the diagnosis and treatment of postmenopausal osteoporosis","volume":"57","author":"Radominski","year":"2017","journal-title":"Rev Bras Reumatol Engl Ed"},{"key":"2025032012274734000_ref3","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.jot.2022.08.003","article-title":"Fecal microbiota transplantation ameliorates bone loss in mice with ovariectomy-induced osteoporosis via modulating gut microbiota and metabolic function","volume":"37","author":"Zhang","year":"2022","journal-title":"J Orthop Translat"},{"key":"2025032012274734000_ref4","doi-asserted-by":"crossref","first-page":"1032614","DOI":"10.3389\/fendo.2022.1032614","article-title":"Necroptosis: a new target for prevention of osteoporosis","volume":"13","author":"Hu","year":"2022","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2025032012274734000_ref5","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1186\/s13018-021-02678-x","article-title":"Effect of drugs on bone mineral density in postmenopausal osteoporosis: a Bayesian network meta-analysis","volume":"16","author":"Migliorini","year":"2021","journal-title":"J Orthop Surg Res"},{"key":"2025032012274734000_ref6","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/B978-0-444-53491-0.00014-6","article-title":"Autonomic control of bone formation: its clinical relevance","volume":"117","author":"Ji-Ye","year":"2013","journal-title":"Handb Clin Neurol"},{"key":"2025032012274734000_ref7","doi-asserted-by":"crossref","first-page":"eaau6977","DOI":"10.1126\/science.aau6977","article-title":"The biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"key":"2025032012274734000_ref8","doi-asserted-by":"crossref","first-page":"R435","DOI":"10.1016\/j.cub.2018.01.059","article-title":"Exosomes and Ectosomes in intercellular communication","volume":"28","author":"Meldolesi","year":"2018","journal-title":"Curr Biol"},{"key":"2025032012274734000_ref9","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/s41368-022-00187-z","article-title":"Exosomes rewire the cartilage microenvironment in osteoarthritis: from intercellular communication to therapeutic strategies","volume":"14","author":"Wu","year":"2022","journal-title":"Int J Oral Sci"},{"key":"2025032012274734000_ref10","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1016\/j.cmet.2021.08.006","article-title":"Exosomes as mediators of intercellular crosstalk in metabolism","volume":"33","author":"Isaac","year":"2021","journal-title":"Cell Metab"},{"key":"2025032012274734000_ref11","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s12265-020-10040-5","article-title":"Cardiovascular exosomes and MicroRNAs in cardiovascular physiology and pathophysiology","volume":"14","author":"Henning","year":"2021","journal-title":"J Cardiovasc Transl Res"},{"key":"2025032012274734000_ref12","doi-asserted-by":"crossref","first-page":"587","DOI":"10.2174\/1570161119666210208202621","article-title":"Exosomes and Exosomal microRNAs in age-associated stroke","volume":"19","author":"Wang","year":"2021","journal-title":"Curr Vasc Pharmacol"},{"key":"2025032012274734000_ref13","doi-asserted-by":"crossref","first-page":"935244","DOI":"10.3389\/fendo.2022.935244","article-title":"Research progress on exosomes\/microRNAs in the treatment of diabetic retinopathy","volume":"13","author":"Niu","year":"2022","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2025032012274734000_ref14","first-page":"1169","article-title":"Collagen VI-related muscle disorders","volume":"63","author":"Higuchi","year":"2011","journal-title":"Brain Nerve"},{"key":"2025032012274734000_ref15","doi-asserted-by":"crossref","first-page":"110560","DOI":"10.1016\/j.cellsig.2022.110560","article-title":"Role of COL6A2 in malignant progression and temozolomide resistance of glioma","volume":"102","author":"Hong","year":"2023","journal-title":"Cell Signal"},{"key":"2025032012274734000_ref16","doi-asserted-by":"crossref","first-page":"5095","DOI":"10.3390\/ijms24065095","article-title":"Collagen VI in the musculoskeletal system","volume":"24","author":"Di Martino","year":"2023","journal-title":"Int J Mol Sci"},{"key":"2025032012274734000_ref17","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/978-94-007-7893-1_12","article-title":"Collagen type VI myopathies","volume":"802","author":"Bushby","year":"2014","journal-title":"Adv Exp Med Biol"},{"key":"2025032012274734000_ref18","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/s13287-018-0955-5","article-title":"The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell self-renewal and multiple differentiation potential","volume":"9","author":"Tu","year":"2018","journal-title":"Stem Cell Res Ther"},{"key":"2025032012274734000_ref19","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1002\/jcb.29355","article-title":"MicroRNA-218-5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells","volume":"121","author":"Kou","year":"2020","journal-title":"J Cell Biochem"},{"key":"2025032012274734000_ref20","doi-asserted-by":"crossref","first-page":"16069","DOI":"10.1038\/nrdp.2016.69","article-title":"Postmenopausal osteoporosis","volume":"2","author":"Eastell","year":"2016","journal-title":"Nat Rev Dis Primers"},{"key":"2025032012274734000_ref21","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/S0736-0266(01)00045-6","article-title":"Early cell adhesion events differ between osteoporotic and non-osteoporotic osteoblasts","volume":"19","author":"Perinpanayagam","year":"2001","journal-title":"J Orthop Res"},{"key":"2025032012274734000_ref22","doi-asserted-by":"crossref","first-page":"403","DOI":"10.33549\/physiolres.932045","article-title":"Adhesion, growth and differentiation of osteoblasts on surface-modified materials developed for bone implants","volume":"60","author":"Vandrovcov\u00e1","year":"2011","journal-title":"Physiol Res"},{"key":"2025032012274734000_ref23","doi-asserted-by":"crossref","first-page":"6366","DOI":"10.1038\/s41467-020-20029-y","article-title":"AN1-type zinc finger protein 3 (ZFAND3) is a transcriptional regulator that drives glioblastoma invasion","volume":"11","author":"Schuster","year":"2020","journal-title":"Nat Commun"},{"key":"2025032012274734000_ref24","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1186\/s13287-021-02214-y","article-title":"Exosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis and enhances adipogenic versus osteogenic differentiation in human bone marrow mesenchymal stem cells by sponging miR-431-5p [published correction appears in stem cell res Ther. 2022 Jul 29;13(1):381. doi: 10.1186\/s13287-022-03096-4]","volume":"12","author":"Zhi","year":"2021","journal-title":"Stem Cell Res Ther"},{"key":"2025032012274734000_ref25","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.3390\/ijms23031376","article-title":"Osteoporosis due to hormone imbalance: an overview of the effects of Estrogen deficiency and glucocorticoid overuse on bone turnover","volume":"23","author":"Cheng","year":"2022","journal-title":"Int J Mol Sci"},{"key":"2025032012274734000_ref26","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/prd.12422","article-title":"Osteoporosis and periodontal diseases - an update on their association and mechanistic links","volume":"89","author":"Yu","year":"2022","journal-title":"Periodontol"},{"key":"2025032012274734000_ref27","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/medu.14380","article-title":"Interpretive description: a flexible qualitative methodology for medical education research","volume":"55","author":"Thompson Burdine","year":"2021","journal-title":"Med Educ"},{"key":"2025032012274734000_ref28","doi-asserted-by":"crossref","first-page":"9724","DOI":"10.3390\/ijms22189724","article-title":"Sarcopenia: Etiology, nutritional approaches, and miRNAs","volume":"22","author":"Cannataro","year":"2021","journal-title":"Int J Mol Sci"},{"key":"2025032012274734000_ref29","doi-asserted-by":"crossref","first-page":"8887","DOI":"10.3390\/ijms21238887","article-title":"The osteoporosis\/microbiota linkage: the role of miRNA","volume":"21","author":"De Martinis","year":"2020","journal-title":"Int J Mol Sci"},{"key":"2025032012274734000_ref30","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1111\/ijlh.13477","article-title":"Epigenetic analysis reveals significant differential expression of miR-378C and miR-128-2-5p in a cohort of relapsed pediatric B-acute lymphoblastic leukemia cases","volume":"43","author":"Bhatia","year":"2021","journal-title":"Int J Lab Hematol"},{"key":"2025032012274734000_ref31","doi-asserted-by":"crossref","first-page":"5283","DOI":"10.1111\/jcmm.16538","article-title":"Baicalein alleviates osteoarthritis by protecting subchondral bone, inhibiting angiogenesis and synovial proliferation","volume":"25","author":"Li","year":"2021","journal-title":"J Cell Mol Med"},{"key":"2025032012274734000_ref32","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1111\/exd.14653","article-title":"The role of dual-specificity phosphatase 3 in melanocytic oncogenesis","volume":"31","author":"Chousakos","year":"2022","journal-title":"Exp Dermatol"},{"key":"2025032012274734000_ref33","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3390\/genes12010055","article-title":"Molecular genetics of glaucoma: subtype and ethnicity considerations","volume":"12","author":"Zukerman","year":"2020","journal-title":"Genes (Basel)"},{"key":"2025032012274734000_ref34","doi-asserted-by":"crossref","first-page":"819056","DOI":"10.3389\/fendo.2022.819056","article-title":"MicroRNAs in serum exosomes as circulating biomarkers for postmenopausal osteoporosis","volume":"13","author":"Shi","year":"2022","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2025032012274734000_ref35","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1186\/s13018-023-04020-z","article-title":"Identification and evaluation of circulating exosomal miRNAs for the diagnosis of postmenopausal osteoporosis","volume":"18","author":"Sun","year":"2023","journal-title":"J Orthop Surg Res"},{"key":"2025032012274734000_ref36","doi-asserted-by":"crossref","first-page":"10125","DOI":"10.18632\/aging.204453","article-title":"Exosomes derived from human umbilical cord mesenchymal stem cells promote osteogenesis through the AKT signaling pathway in postmenopausal osteoporosis [retracted in: aging (Albany NY). 2023 Dec 14;15(23):14506. doi: 10.18632\/aging.205437]","volume":"14","author":"Ren","year":"2022","journal-title":"Aging (Albany NY)"},{"key":"2025032012274734000_ref37","doi-asserted-by":"crossref","first-page":"e23916","DOI":"10.1002\/jcla.23916","article-title":"Microarray analysis of circRNAs sequencing profile in exosomes derived from bone marrow mesenchymal stem cells in postmenopausal osteoporosis patients","volume":"36","author":"Fu","year":"2022","journal-title":"J Clin Lab Anal"},{"key":"2025032012274734000_ref38","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.1002\/cbin.11690","article-title":"M1 macrophage-derived exosomes aggravate bone loss in postmenopausal osteoporosis via a microRNA-98\/DUSP1\/JNK axis","volume":"45","author":"Yu","year":"2021","journal-title":"Cell Biol Int"},{"key":"2025032012274734000_ref39","doi-asserted-by":"crossref","first-page":"4231","DOI":"10.1002\/jcp.30179","article-title":"The emerging role of miR-128 in musculoskeletal diseases","volume":"236","author":"Shang","year":"2021","journal-title":"J Cell Physiol"}],"container-title":["Human Molecular Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/hmg\/article-pdf\/34\/7\/563\/61457974\/ddae147.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/hmg\/article-pdf\/34\/7\/563\/61457974\/ddae147.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T23:52:14Z","timestamp":1742514734000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/hmg\/article\/34\/7\/563\/7954543"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,16]]},"references-count":39,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,1,16]]},"published-print":{"date-parts":[[2025,3,20]]}},"URL":"https:\/\/doi.org\/10.1093\/hmg\/ddae147","relation":{},"ISSN":["0964-6906","1460-2083"],"issn-type":[{"value":"0964-6906","type":"print"},{"value":"1460-2083","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,4,1]]},"published":{"date-parts":[[2025,1,16]]}}}