{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:47:27Z","timestamp":1789984047700,"version":"4.0.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"13","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Natural Science Foundation of Fujian Province of China","award":["2021\u00a0J01411"],"award-info":[{"award-number":["2021\u00a0J01411"]}]},{"name":"Joint Funds for the innovation of science and Technology, Fujian province","award":["2020Y9158"],"award-info":[{"award-number":["2020Y9158"]}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["82170718"],"award-info":[{"award-number":["82170718"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["81270766"],"award-info":[{"award-number":["81270766"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mutations in four genes encoding the outer ring complex of nuclear pore complexes (NPCs), NUP85, NUP107, NUP133 and NUP160, cause monogenic steroid-resistant nephrotic syndrome (SRNS). Knockout of NUP85, NUP107, or NUP133 in immortalized human podocytes activates CDC42, an important effector of SRNS pathogenesis. However, it is unknown whether or not loss of NUP160 dysregulates CDC42 in the podocytes. Here, we generated a podocyte-specific Nup160 knockout mouse model with double-fluorescent (mT\/mG) Cre reporter genes using CRISPR\/Cas9 and Cre\/loxP technologies. We investigated nephrotic syndrome-associated phenotypes in the Nup160podo\u2212\/\u2212 mice, and performed single-cell transcriptomic and proteomic analysis of glomerular suspension cells and cultured primary podocytes, respectively. The Nup160podo\u2212\/\u2212 mice exhibited progressive proteinuria and fusion of podocyte foot processes. We found decreased Cdc42 protein and normal Cdc42 transcriptional level in the podocytes of the Nup160podo\u2212\/\u2212 mice using analysis of single-cell transcriptomes and proteomes. We subsequently observed that Cdc42 protein decreased in both kidney tissues and cultured primary podocytes of the Nup160podo\u2212\/\u2212 mice, although Cdc42 mRNA levels were elevated in the cultured primary podocytes of the Nup160podo\u2212\/\u2212 mice. We also found that Cdc42 activity was significantly reduced in the cultured primary podocytes of the Nup160podo\u2212\/\u2212 mice. In conclusion, loss of Nup160 dysregulated Cdc42 in the podocytes of the Nup160podo\u2212\/\u2212 mice with proteinuria and fusion of podocyte foot processes. Our findings suggest that the dysregulation of CDC42 may contribute to the pathogenesis of SRNS in patients with mutations in NUP160.<\/jats:p>","DOI":"10.1093\/hmg\/ddaf064","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T12:01:40Z","timestamp":1745928100000},"page":"1134-1145","source":"Crossref","is-referenced-by-count":5,"title":["Loss of <i>Nup160<\/i> dysregulates Cdc42 in the podocytes of podocyte-specific <i>Nup160<\/i> knockout mice"],"prefix":"10.1093","volume":"34","author":[{"given":"Deying","family":"Liu","sequence":"first","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Medical Research Center, Fujian Maternity and Child Health Hospital , 19 Jin Ji Shan Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Medical Research Center, Fujian Maternity and Child Health Hospital , 19 Jin Ji Shan Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Medical Research Center, Fujian Maternity and Child Health Hospital , 19 Jin Ji Shan Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Medical Research Center, Fujian Maternity and Child Health Hospital , 19 Jin Ji Shan Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Medical Research Center, Fujian Maternity and Child Health Hospital , 19 Jin Ji Shan Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huajuan","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojian","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihua","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian Maternity and Child Health Hospital , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Department of Nephrology, Rheumatology and Immunology, Fujian Children\u2019s Hospital , 966 Heng Yu Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Medical Research Center, Fujian Maternity and Child Health Hospital , 19 Jin Ji Shan Road, Fuzhou, Fujian 350014 ,","place":["China"]},{"name":"Fujian Key Laboratory of Women and Children\u2019s Critical Diseases Research , 18 Daoshan Road, Fuzhou, Fujian 350000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"2025062609271255300_ref1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0140-6736(18)30536-1","article-title":"Idiopathic nephrotic syndrome in children","volume":"392","author":"Noone","year":"2018","journal-title":"Lancet"},{"key":"2025062609271255300_ref2","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1093\/ndt\/gfaa221","article-title":"The genetics of steroid-resistant nephrotic syndrome in children","volume":"37","author":"Dorval","year":"2022","journal-title":"Nephrol Dial Transplant"},{"key":"2025062609271255300_ref3","first-page":"371","article-title":"Podocyte injury and its consequences","volume":"20","author":"Nagata","year":"2016","journal-title":"Nat Rev Nephrol"},{"key":"2025062609271255300_ref4","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/s41581-024-00815-3","article-title":"Role of biophysics and mechanobiology in podocyte physiology","volume":"20","author":"Haydak","year":"2024","journal-title":"Nat Rev Nephrol"},{"key":"2025062609271255300_ref5","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s41572-020-0196-7","article-title":"Podocytopathies","volume":"6","author":"Kopp","year":"2020","journal-title":"Nat Rev Dis Primers"},{"key":"2025062609271255300_ref6","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1681\/ASN.2018080786","article-title":"Mutations in NUP160 are implicated in steroid-resistant nephrotic syndrome","volume":"30","author":"Zhao","year":"2019","journal-title":"J Am Soc Nephrol"},{"key":"2025062609271255300_ref7","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1007\/s00467-024-06494-3","article-title":"Nucleoporin-associated steroid-resistant nephrotic syndrome","volume":"40","author":"Yao","year":"2024","journal-title":"Pediatr Nephrol"},{"key":"2025062609271255300_ref8","first-page":"399","article-title":"Homozygous mutation in NUP107 leads to microcephaly with steroid-resistant nephrotic condition similar to Galloway-Mowat syndrome","volume":"54","author":"Rosti","year":"2017","journal-title":"Hum Mol Genet"},{"key":"2025062609271255300_ref9","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/ng.3512","article-title":"Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome","volume":"48","author":"Braun","year":"2016","journal-title":"Nat 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