{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T02:45:41Z","timestamp":1782873941009,"version":"3.54.5"},"reference-count":78,"publisher":"Oxford University Press (OUP)","issue":"24","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes for Health Research","doi-asserted-by":"crossref","award":["PJT 186235"],"award-info":[{"award-number":["PJT 186235"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The long non-coding RNA XIST is critical for establishing X-chromosome inactivation early in development. XIST remains expressed from the inactive X throughout female life; yet in somatic cells silencing of most genes continues in the absence of XIST. As changes in XIST expression are observed in cancers and aging we sought to examine the impact of complete or partial XIST loss on gene expression and chromatin structure in the hTERT RPE-1 cell line. XIST regulatory elements lie within the gene, with ongoing expression dependent on the A repeat region. Upon loss of XIST expression there was up-regulation of a subset of X-linked genes, with escape genes showing an average 20% increase in expression. Near full Xi-expression was found for MED14 and USP9X upon XIST loss, with partial up-regulation observed with the reduced XIST expression caused by removal of the F-repeat containing region or XIST delocalization following deletion of the E repeat region. Comparison with other studies of XIST loss in human cell lines revealed recurrent impact on escapees, while autosomal genes demonstrated no consistent XIST-dependencies. Loss of XIST in hTERT RPE-1 resulted in loss of H3K27me3 and H2AK119ub; however, independent inhibition of these pathways failed to upregulate MED14 and USP9X. In contrast, reduction of H3K9me3 caused partial reactivation of USP9X but not MED14, thus implicating a combinatorial action of silencing pathways in X-chromosome inactivation. Our work, in agreement with previous studies, reveals that in somatic cells XIST expression and localization suppresses the expression of escape genes.<\/jats:p>","DOI":"10.1093\/hmg\/ddaf160","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T12:13:05Z","timestamp":1759925585000},"page":"2027-2041","source":"Crossref","is-referenced-by-count":5,"title":["Impact of somatic\n                    <i>XIST<\/i>\n                    deletions on ongoing XIST expression and inactive X silencing and heterochromatin"],"prefix":"10.1093","volume":"34","author":[{"given":"Karanveer S","family":"Bhangu","sequence":"first","affiliation":[{"name":"Department of Medical Genetics, University of British Columbia , 2350 Health Sciences Mall, Vancouver, BC Canada V6T 1Z3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of British Columbia , 2350 Health Sciences Mall, Vancouver, BC Canada V6T 1Z3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah J","family":"Illing","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of British Columbia , 2350 Health Sciences Mall, Vancouver, BC Canada V6T 1Z3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8959-0101","authenticated-orcid":false,"given":"Carolyn J","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of British Columbia , 2350 Health Sciences Mall, Vancouver, BC Canada V6T 1Z3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"2025120606492589100_ref1","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1038\/190372a0","article-title":"Gene action in the X-chromosome of the mouse (Mus musculus 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