{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T21:46:08Z","timestamp":1790545568809,"version":"4.1.0"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,1,15]],"date-time":"2020-01-15T00:00:00Z","timestamp":1579046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","award":["30636"],"award-info":[{"award-number":["30636"]}],"id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["148756"],"award-info":[{"award-number":["148756"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CGS-M Frederick Banting and Charles Best Canada Graduate Scholarship"},{"DOI":"10.13039\/100012285","name":"BC Children's Hospital Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005247","name":"University of British Columbia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005247","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mutations in each of the four human VPS13 (VPS13A\u2013D) proteins are associated with distinct neurological disorders: chorea-acanthocytosis, Cohen syndrome, early-onset Parkinson\u2019s disease and spastic ataxia. Recent evidence suggests that the different VPS13 paralogs transport lipids between organelles at different membrane contact sites. How each VPS13 isoform is targeted to organelles is not known. We have shown that the localization of yeast Vps13 protein to membranes requires a conserved six-repeat region, the Vps13 Adaptor Binding (VAB) domain, which binds to organelle-specific adaptors. Here, we use a systematic mutagenesis strategy to determine the role of each repeat in recognizing each known adaptor. Our results show that mutation of invariant asparagines in repeats 1 and 6 strongly impacts the binding of all adaptors and blocks Vps13 membrane recruitment. However, we find that repeats 5\u20136 are sufficient for localization and interaction with adaptors. This supports a model where a single adaptor-binding site is found in the last two repeats of the VAB domain, while VAB domain repeat 1 may influence domain conformation. Importantly, a disease-causing mutation in VPS13D, which maps to the highly conserved asparagine residue in repeat 6, blocks adaptor binding and Vps13 membrane recruitment when modeled in yeast. Our findings are consistent with a conserved adaptor binding role for the VAB domain and suggest the presence of as-yet-unidentified adaptors in both yeast and humans.<\/jats:p>","DOI":"10.1093\/hmg\/ddz318","type":"journal-article","created":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T15:09:38Z","timestamp":1578668978000},"page":"635-648","source":"Crossref","is-referenced-by-count":43,"title":["A VPS13D spastic ataxia mutation disrupts the conserved adaptor-binding site in yeast Vps13"],"prefix":"10.1093","volume":"29","author":[{"given":"Samantha K","family":"Dziurdzik","sequence":"first","affiliation":[{"name":"Centre for Molecular Medicine and Therapeutics, British Columbia Children\u2019s Hospital Research Institute, University of British Columbia, Vancouver, BC V5Z 4H4, Canada"},{"name":"Department of Medical Genetics, University of British Columbia, Vancouver, BC V6H 3N1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn D M","family":"Bean","sequence":"first","affiliation":[{"name":"Department of Biology, Concordia University, Montreal, QC H3G 1M8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Davey","sequence":"first","affiliation":[{"name":"Centre for Molecular Medicine and Therapeutics, British Columbia Children\u2019s Hospital Research Institute, University of British Columbia, Vancouver, BC V5Z 4H4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Conibear","sequence":"first","affiliation":[{"name":"Centre for Molecular Medicine and Therapeutics, British Columbia Children\u2019s Hospital Research Institute, University of British Columbia, Vancouver, BC V5Z 4H4, Canada"},{"name":"Department of Medical Genetics, University of British Columbia, Vancouver, BC V6H 3N1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,15]]},"reference":[{"key":"2020031302572223300_ref1","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1038\/s41467-019-09253-3","article-title":"Coming together to define membrane contact sites","volume":"10","author":"Scorrano","year":"2019","journal-title":"Nat. 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Genet."},{"key":"2020031302572223300_ref8","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1086\/375454","article-title":"Cohen syndrome is caused by mutations in a novel gene, COH1, encoding a transmembrane protein with a presumed role in vesicle-mediated sorting and intracellular protein transport","volume":"72","author":"Kolehmainen","year":"2003","journal-title":"Am. J. Hum. Genet."},{"key":"2020031302572223300_ref9","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.neuron.2012.11.002","article-title":"Using whole-exome sequencing to identify inherited causes of autism","volume":"77","author":"Yu","year":"2013","journal-title":"Neuron"},{"key":"2020031302572223300_ref10","doi-asserted-by":"crossref","first-page":"434","DOI":"10.3389\/fgene.2019.00434","article-title":"Comprehensive analysis of rare variants of 101 autism-linked genes in a Hungarian cohort of autism spectrum disorder patients","volume":"10","author":"Balicza","year":"2019","journal-title":"Front. Genet."},{"key":"2020031302572223300_ref11","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.ajhg.2016.01.014","article-title":"Loss of VPS13C function in autosomal-recessive parkinsonism causes mitochondrial dysfunction and increases PINK1 \/ parkin-dependent mitophagy","volume":"98","author":"Lesage","year":"2016","journal-title":"Am. J. Hum. 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