{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:05:56Z","timestamp":1791651956630,"version":"4.3.4"},"reference-count":57,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The genus Camelina (Brassicaceae) comprises 7\u20138 diploid, tetraploid, and hexaploid species. Of particular agricultural interest is the biofuel crop, C. sativa (gold-of-pleasure or false flax), an allohexaploid domesticated from the widespread weed, C. microcarpa. Recent cytogenetics and genomics work has uncovered the identity of the parental diploid species involved in ancient polyploidization events in Camelina. However, little is known about the maternal subgenome ancestry of contemporary polyploid species. To determine the diploid maternal contributors of polyploid Camelina lineages, we sequenced and assembled 84 Camelina chloroplast genomes for phylogenetic analysis. Divergence time estimation was used to infer the timing of polyploidization events. Chromosome counts were also determined for 82 individuals to assess ploidy and cytotypic variation. Chloroplast genomes showed minimal divergence across the genus, with no observed gene-loss or structural variation. Phylogenetic analyses revealed C. hispida as a maternal diploid parent to the allotetraploid Camelina rumelica, and C. neglecta as the closest extant diploid contributor to the allohexaploids C. microcarpa and C. sativa. The tetraploid C. rumelica appears to have evolved through multiple independent hybridization events. Divergence times for polyploid lineages closely related to C. sativa were all inferred to be very recent, at only ~65 thousand years ago. Chromosome counts confirm that there are two distinct cytotypes within C. microcarpa (2n\u2009=\u200938 and 2n\u2009=\u200940). Based on these findings and other recent research, we propose a model of Camelina subgenome relationships representing our current understanding of the hybridization and polyploidization history of this recently-diverged genus.<\/jats:p>","DOI":"10.1093\/hr\/uhab050","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T04:17:00Z","timestamp":1641961020000},"source":"Crossref","is-referenced-by-count":35,"title":["Chloroplast phylogenomics in\n                    <i>Camelina<\/i>\n                    (Brassicaceae) reveals multiple origins of polyploid species and the maternal lineage of\n                    <i>C. sativa<\/i>"],"prefix":"10.1093","volume":"9","author":[{"given":"Jordan R","family":"Brock","sequence":"first","affiliation":[{"name":"Washington University in St. Louis Department of Biology, , St. Louis, Missouri, 63130 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terezie","family":"Mand\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Masaryk University CEITEC \u2013 Central European Institute of Technology, , Kamenice 5, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"McKain","sequence":"additional","affiliation":[{"name":"The University of Alabama Department of Biological Sciences, , 411 Mary Harmon Bryant Hall, Tuscaloosa, Alabama, 35487 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin A","family":"Lysak","sequence":"additional","affiliation":[{"name":"Masaryk University CEITEC \u2013 Central European Institute of Technology, , Kamenice 5, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth M","family":"Olsen","sequence":"additional","affiliation":[{"name":"Washington University in St. Louis Department of Biology, , St. Louis, Missouri, 63130 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,1,5]]},"reference":[{"key":"2024071111382171800_ref1","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/j.1467-7652.2010.00558.x","article-title":"Chloroplast genome sequences from total DNA for plant identification","volume":"9","author":"Nock","year":"2011","journal-title":"Plant Biotechnol J"},{"key":"2024071111382171800_ref2","first-page":"111","article-title":"A phylogenetic analysis of chloroplast genomes elucidates the relationships of the six economically important brassica species comprising the triangle of U","volume":"8","author":"Li","year":"2017","journal-title":"Front Plant Sci"},{"key":"2024071111382171800_ref3","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/1471-2229-10-54","article-title":"Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers","volume":"10","author":"Allender","year":"2010","journal-title":"BMC Plant Biol"},{"key":"2024071111382171800_ref4","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1139\/g02-033","article-title":"Ascertaining maternal and paternal lineage within Musa by chloroplast and mitochondrial DNA RFLP analyses","volume":"45","author":"Carreel","year":"2002","journal-title":"Genome"},{"key":"2024071111382171800_ref5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0085761","article-title":"Sequencing of chloroplast genomes from wheat, barley, rye and their relatives provides a detailed insight into the evolution of the Triticeae tribe","volume":"9","author":"Middleton","year":"2014","journal-title":"PLoS One"},{"key":"2024071111382171800_ref6","doi-asserted-by":"crossref","first-page":"4132","DOI":"10.1073\/pnas.86.11.4132","article-title":"New World tetraploid cottons contain Old World cytoplasm","volume":"86","author":"Wendel","year":"1989","journal-title":"Proc 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(Brassicaceae)","volume":"14","author":"Ceplitis","year":"2005","journal-title":"Mol Ecol"},{"key":"2024071111382171800_ref10","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.ympev.2011.08.023","article-title":"Chloroplast DNA phylogeography of a distylous shrub (Palicourea padifolia, Rubiaceae) reveals past fragmentation and demographic expansion in Mexican cloud forests","volume":"61","author":"Guti\u00e9rrez-Rodr\u00edguez","year":"2011","journal-title":"Mol Phylogenet Evol"},{"key":"2024071111382171800_ref11","article-title":"Robust DNA isolation and high-throughput sequencing library construction for herbarium specimens. 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