{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T02:56:49Z","timestamp":1781665009418,"version":"3.54.5"},"reference-count":80,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study","award":["SN-ZJU-SIAS-0011"],"award-info":[{"award-number":["SN-ZJU-SIAS-0011"]}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang province","doi-asserted-by":"publisher","award":["LZ22C150005"],"award-info":[{"award-number":["LZ22C150005"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Research and Development Project of China","award":["2024YFD2001003"],"award-info":[{"award-number":["2024YFD2001003"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472718"],"award-info":[{"award-number":["32472718"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The transition of plants from aquatic to terrestrial environments required effective barriers against water loss and UV damage. The plant cuticle, a hydrophobic barrier covering aerial surfaces, emerged as a critical innovation, yet how its biosynthesis is regulated in specialized structures remains poorly understood. This study identifies two long-chain acyl-CoA synthetases, SlLACS1 and SlLACS2, that exhibit both distinct and overlapping functions in cuticle formation across tomato tissues. These genes show striking specificity in different trichome types: SlLACS1 functions in type I\/IV trichomes, while SlLACS2 is required for type VI trichome cuticle integrity. However, they act redundantly in leaf epidermal and fruit cuticle formation, as revealed by analysis of single and double mutants. Unexpectedly, simultaneous disruption of both genes severely compromises pollen viability through defective pollen coat formation. Biochemical characterization demonstrates that SlLACS1 and SlLACS2 maintain their ancestral enzymatic function of activating long-chain fatty acids, an activity conserved from algal LACS homologs. These findings reveal how gene duplication and diversification facilitated the development of specialized hydrophobic barrier functions in distinct tissues while maintaining redundancy in fundamental protective structures, representing a sophisticated adaptation to terrestrial life.<\/jats:p>","DOI":"10.1093\/hr\/uhaf114","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T06:29:19Z","timestamp":1745821759000},"source":"Crossref","is-referenced-by-count":3,"title":["Evolutionary diversification of acyl-CoA synthetases underpins hydrophobic barrier formation across diverse tomato tissues and beyond"],"prefix":"10.1093","volume":"12","author":[{"given":"Jianfeng","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Horticulture, Zijingang Campus, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyu","family":"He","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Zijingang Campus, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyi","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Zijingang Campus, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Zijingang Campus, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Lyu","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Zijingang Campus, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinheng","family":"Pan","sequence":"additional","affiliation":[{"name":"Mass Spectrometry & Metabolomics Core Facility, The Biomedical Research Core Facility, Westlake University , Hangzhou 310030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarong","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Feng","sequence":"additional","affiliation":[{"name":"Mass Spectrometry & Metabolomics Core Facility, The Biomedical Research Core Facility, Westlake University , Hangzhou 310030 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing-xing","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingquan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Zijingang Campus, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L","family":"Last","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Michigan State University , East Lansing, MI 48824 ,","place":["USA"]},{"name":"Department of Plant Biology, Michigan State University , East Lansing, MI 48824 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengxiang","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Zijingang Campus, Zhejiang University , Hangzhou 310058 ,","place":["China"]},{"name":"Key Laboratory of Horticultural Plants Growth and Development, Agricultural Ministry of China , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"key":"2025071109313234100_ref1","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.tplants.2022.09.009","article-title":"The origin and early evolution of plants","volume":"28","author":"Bowles","year":"2023","journal-title":"Trends Plant Sci"},{"key":"2025071109313234100_ref2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1146\/annurev.ecolsys.39.110707.173526","article-title":"The earliest land plants","volume":"39","author":"Gensel","year":"2008","journal-title":"Annu Rev Ecol Evol Syst"},{"key":"2025071109313234100_ref3","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/37918","article-title":"The origin and early evolution of plants on land","volume":"389","author":"Kenrick","year":"1997","journal-title":"Nature"},{"key":"2025071109313234100_ref4","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1095-8339.1984.tb01566.x","article-title":"Physiological correlates of the morphology of early vascular plants","volume":"88","author":"Raven","year":"1984","journal-title":"Bot J Linn Soc"},{"key":"2025071109313234100_ref5","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1111\/jipb.13224","article-title":"Origin and evolution of green plants in the light of key evolutionary events","volume":"64","author":"Zhang","year":"2022","journal-title":"J Integr Plant Biol"},{"key":"2025071109313234100_ref6","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/jpy.12180","article-title":"Detection of flavonoids in microalgae from different evolutionary lineages (M Posewitz, Ed.)","volume":"50","author":"Goiris","year":"2014","journal-title":"J Phycol"},{"key":"2025071109313234100_ref7","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.1104\/pp.20.00913","article-title":"Origins and evolution of cuticle biosynthetic machinery in land plants","volume":"184","author":"Kong","year":"2020","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref8","doi-asserted-by":"crossref","first-page":"R210","DOI":"10.1016\/j.cub.2023.01.003","article-title":"The plant cuticle","volume":"33","author":"Gonz\u00e1lez-Valenzuela","year":"2023","journal-title":"Curr Biol"},{"key":"2025071109313234100_ref9","doi-asserted-by":"crossref","first-page":"R1281","DOI":"10.1016\/j.cub.2021.07.038","article-title":"The evolutionary emergence of land plants","volume":"31","author":"Donoghue","year":"2021","journal-title":"Curr Biol"},{"key":"2025071109313234100_ref10","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1093\/jxb\/52.suppl_1.381","article-title":"Roots: evolutionary origins and biogeochemical significance","volume":"52","author":"Raven","year":"2001","journal-title":"J Exp Bot"},{"key":"2025071109313234100_ref11","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1093\/plphys\/kiac304","article-title":"Deep origin and gradual evolution of transporting tissues: perspectives from across the land plants","volume":"190","author":"Woudenberg","year":"2022","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref12","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1038\/s41477-022-01283-y","article-title":"The origin of a land flora","volume":"8","author":"Bowman","year":"2022","journal-title":"Nat Plants"},{"key":"2025071109313234100_ref13","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.palaeo.2005.07.006","article-title":"Cretaceous angiosperm flowers: innovation and evolution in plant reproduction","volume":"232","author":"Friis","year":"2006","journal-title":"Palaeogeogr Palaeoclimatol Palaeoecol"},{"key":"2025071109313234100_ref14","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1126\/science.abi6984","article-title":"Reproductive innovations and pulsed rise in plant complexity","volume":"373","author":"Leslie","year":"2021","journal-title":"Science"},{"key":"2025071109313234100_ref15","first-page":"716","article-title":"Plant secondary metabolites: the weapons for biotic stress management","volume":"13","author":"Al-Khayri","year":"2023","journal-title":"Meta"},{"key":"2025071109313234100_ref16","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1104\/pp.17.00727","article-title":"Plant glandular trichomes: natural cell factories of high biotechnological interest","volume":"175","author":"Huchelmann","year":"2017","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref17","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s40529-023-00370-3","article-title":"Morphological characterization of intraspecific variation for trichome traits in tomato (Solanum lycopersicum)","volume":"64","author":"Kaur","year":"2023","journal-title":"Bot Stud"},{"key":"2025071109313234100_ref18","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/978-981-19-5779-6_15","volume-title":"Phytochemical Genomics: Plant Metabolomics and Medicinal Plant Genomics","author":"Dhankhar","year":"2022"},{"key":"2025071109313234100_ref19","doi-asserted-by":"crossref","first-page":"17077","DOI":"10.3390\/ijms131217077","article-title":"Plant glandular trichomes as targets for breeding or engineering of resistance to herbivores","volume":"13","author":"Glas","year":"2012","journal-title":"Int J Mol Sci"},{"key":"2025071109313234100_ref20","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1111\/j.1365-313X.2008.03432.x","article-title":"Harnessing plant trichome biochemistry for the production of useful compounds","volume":"54","author":"Schilmiller","year":"2008","journal-title":"Plant J"},{"key":"2025071109313234100_ref21","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1111\/j.1365-313X.2010.04154.x","article-title":"Mass spectrometry screening reveals widespread diversity in trichome specialized metabolites of tomato chromosomal substitution lines","volume":"62","author":"Schilmiller","year":"2010","journal-title":"Plant J"},{"key":"2025071109313234100_ref22","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/j.1365-313X.2009.03969.x","article-title":"Cutin deficiency in the tomato fruit cuticle consistently affects resistance to microbial infection and biomechanical properties, but not transpirational water loss","volume":"60","author":"Isaacson","year":"2009","journal-title":"Plant J"},{"key":"2025071109313234100_ref23","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1093\/jxb\/erad410","article-title":"NAC transcription factor SlNOR-like1 plays a dual regulatory role in tomato fruit cuticle formation","volume":"75","author":"Liu","year":"2023","journal-title":"J Exp Bot"},{"key":"2025071109313234100_ref24","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/tpj.12422","article-title":"Tomato Cutin deficient 1 (CD1) and putative orthologs comprise an ancient family of cutin synthase-like (CUS) proteins that are conserved among land plants","volume":"77","author":"Yeats","year":"2014","journal-title":"Plant J"},{"key":"2025071109313234100_ref25","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/nph.20009","article-title":"Revisiting plant cuticle biophysics","volume":"244","author":"Heredia","year":"2024","journal-title":"New Phytol"},{"key":"2025071109313234100_ref26","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1104\/pp.113.222737","article-title":"The formation and function of plant cuticles","volume":"163","author":"Yeats","year":"2013","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref27","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1146\/annurev-arplant-043015-111929","article-title":"The plant polyester cutin: biosynthesis, structure, and biological roles","volume":"67","author":"Fich","year":"2016","journal-title":"Annu Rev Plant Biol"},{"key":"2025071109313234100_ref28","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1111\/j.1365-313X.2009.03892.x","article-title":"Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis","volume":"59","author":"L\u00fc","year":"2009","journal-title":"Plant J"},{"key":"2025071109313234100_ref29","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1469-8137.2010.03514.x","article-title":"Epidermis: the formation and functions of a fundamental plant tissue","volume":"189","author":"Javelle","year":"2011","journal-title":"New Phytol"},{"key":"2025071109313234100_ref30","doi-asserted-by":"crossref","first-page":"3119","DOI":"10.1105\/tpc.112.101055","article-title":"Tomato GDSL1 is required for cutin deposition in the fruit cuticle","volume":"24","author":"Girard","year":"2012","journal-title":"Plant Cell"},{"key":"2025071109313234100_ref31","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1104\/pp.112.198374","article-title":"Pleiotropic phenotypes of the sticky peel mutant provide new insight into the role of CUTIN DEFICIENT2 in epidermal cell function in tomato","volume":"159","author":"Nadakuduti","year":"2012","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref32","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/tpj.14733","article-title":"WOOLLY, interacting with MYB transcription factor MYB31, regulates cuticular wax biosynthesis by modulating CER6 expression in tomato","volume":"103","author":"Xiong","year":"2020","journal-title":"Plant J"},{"key":"2025071109313234100_ref33","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1038\/nchembio.960","article-title":"The identification of cutin synthase: formation of the plant polyester cutin","volume":"8","author":"Yeats","year":"2012","journal-title":"Nat Chem Biol"},{"key":"2025071109313234100_ref34","doi-asserted-by":"crossref","first-page":"19499","DOI":"10.3390\/ijms151119499","article-title":"Over-expression of SlSHN1 gene improves drought tolerance by increasing cuticular wax accumulation in tomato","volume":"15","author":"Al-Abdallat","year":"2014","journal-title":"Int J Mol Sci"},{"key":"2025071109313234100_ref35","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1105\/tpc.16.00490","article-title":"MYB107 and MYB9 homologs regulate suberin deposition in angiosperms","volume":"28","author":"Lashbrooke","year":"2016","journal-title":"Plant Cell"},{"key":"2025071109313234100_ref36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1046\/j.1365-313X.2003.01946.x","article-title":"A new method for rapid visualization of defects in leaf cuticle reveals five intrinsic patterns of surface defects in Arabidopsis","volume":"37","author":"Tanaka","year":"2004","journal-title":"Plant J"},{"key":"2025071109313234100_ref37","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1104\/pp.109.145813","article-title":"TrichOME: a comparative omics database for plant trichomes","volume":"152","author":"Dai","year":"2010","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref38","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.pbi.2019.03.005","article-title":"Tip of the trichome: evolution of acylsugar metabolic diversity in Solanaceae","volume":"49","author":"Fan","year":"2019","journal-title":"Curr Opin Plant Biol"},{"key":"2025071109313234100_ref39","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1111\/pce.14530","article-title":"The overlooked functions of trichomes: water absorption and metal detoxication","volume":"46","author":"Li","year":"2023","journal-title":"Plant Cell Environ"},{"key":"2025071109313234100_ref40","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/aob\/mcv065","article-title":"Hydathode trichomes actively secreting water from leaves play a key role in the physiology and evolution of root-parasitic rhinanthoid Orobanchaceae","volume":"116","author":"Sv\u011btl\u00edkov\u00e1","year":"2015","journal-title":"Ann Bot"},{"key":"2025071109313234100_ref41","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.1104\/pp.003269","article-title":"Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism","volume":"129","author":"Shockey","year":"2002","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref42","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/s41467-017-02782-9","article-title":"High-resolution spatiotemporal transcriptome mapping of tomato fruit development and ripening","volume":"9","author":"Shinozaki","year":"2018","journal-title":"Nat Commun"},{"key":"2025071109313234100_ref43","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1146\/annurev-arplant-042809-112312","article-title":"Genetic regulation of sporopollenin synthesis and pollen exine development","volume":"62","author":"Ariizumi","year":"2011","journal-title":"Annu Rev Plant Biol"},{"key":"2025071109313234100_ref44","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1038\/s41477-023-01413-0","article-title":"Biosynthesis and transport of pollen coat precursors in angiosperms","volume":"9","author":"Qiao","year":"2023","journal-title":"Nat Plants"},{"key":"2025071109313234100_ref45","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.134438","article-title":"Identification of long-chain acyl-CoA synthetase gene family reveals that SlLACS1 is essential for cuticular wax biosynthesis in tomato","volume":"277","author":"Wu","year":"2024","journal-title":"Int J Biol Macromol"},{"key":"2025071109313234100_ref46","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.ympev.2003.07.018","article-title":"Molecular adaptation and the origin of land plants","volume":"29","author":"Waters","year":"2003","journal-title":"Mol Phylogenet Evol"},{"key":"2025071109313234100_ref47","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1126\/science.1150646","article-title":"The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants","volume":"319","author":"Rensing","year":"2008","journal-title":"Science"},{"key":"2025071109313234100_ref48","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.1111\/nph.17827","article-title":"Subtle interplay between trichome development and cuticle formation in plants","volume":"233","author":"Berhin","year":"2022","journal-title":"New Phytol"},{"key":"2025071109313234100_ref49","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1016\/j.tplants.2017.09.001","article-title":"Plant volatiles: going \u2018in\u2019 but not \u2018out\u2019 of trichome cavities","volume":"22","author":"Tissier","year":"2017","journal-title":"Trends Plant Sci"},{"key":"2025071109313234100_ref50","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.tplants.2015.06.009","article-title":"Rethinking how volatiles are released from plant cells","volume":"20","author":"Widhalm","year":"2015","journal-title":"Trends Plant Sci"},{"key":"2025071109313234100_ref51","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1104\/pp.114.242040","article-title":"Wettability, polarity, and water absorption of holm oak leaves: effect of leaf side and age","volume":"166","author":"Fern\u00e1ndez","year":"2014","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref52","doi-asserted-by":"crossref","first-page":"1906","DOI":"10.1111\/nph.17336","article-title":"Domino-like water transport on tillandsia through flexible trichome wings","volume":"231","author":"Ha","year":"2021","journal-title":"New Phytol"},{"key":"2025071109313234100_ref53","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1071\/FP16095","article-title":"Ecophysiology of leaf trichomes","volume":"43","author":"Bickford","year":"2016","journal-title":"Funct Plant Biol"},{"key":"2025071109313234100_ref54","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1111\/j.1438-8677.1989.tb00070.x","article-title":"Gas exchange and water vapor uptake in the atmospheric CAM bromeliad Tillandsia recurvata L.: the influence of trichomes","volume":"102","author":"Schmitt","year":"1989","journal-title":"Botanica Acta"},{"key":"2025071109313234100_ref55","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1146\/annurev-arplant-080620-021907","article-title":"Reproduction multitasking: the male gametophyte","volume":"72","author":"Hafidh","year":"2021","journal-title":"Annu Rev Plant Biol"},{"key":"2025071109313234100_ref56","doi-asserted-by":"crossref","first-page":"S84","DOI":"10.1105\/tpc.015800","article-title":"Pollen and stigma structure and function: the role of diversity in pollination","volume":"16","author":"Edlund","year":"2004","journal-title":"Plant Cell"},{"key":"2025071109313234100_ref57","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.flora.2005.02.006","article-title":"Pollenkitt \u2013 its composition, forms and functions","volume":"200","author":"Pacini","year":"2005","journal-title":"Flora"},{"key":"2025071109313234100_ref58","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat Methods"},{"key":"2025071109313234100_ref59","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat Biotechnol"},{"key":"2025071109313234100_ref60","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1186\/1471-2105-9-559","article-title":"WGCNA: an R package for weighted correlation network analysis","volume":"9","author":"Langfelder","year":"2008","journal-title":"BMC Bioinformatics"},{"key":"2025071109313234100_ref61","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1093\/bioinformatics\/btm563","article-title":"Defining clusters from a hierarchical cluster tree: the dynamic tree cut package for R","volume":"24","author":"Langfelder","year":"2008","journal-title":"Bioinformatics"},{"key":"2025071109313234100_ref62","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1104\/pp.107.107391","article-title":"A ligation-independent cloning tobacco rattle virus vector for high-throughput virus-induced gene silencing identifies roles for NbMADS4-1 and \u22122 in floral development","volume":"145","author":"Dong","year":"2007","journal-title":"Plant Physiol"},{"key":"2025071109313234100_ref63","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1046\/j.1365-313X.2002.01394.x","article-title":"Virus-induced gene silencing in tomato","volume":"31","author":"Liu","year":"2002","journal-title":"Plant J"},{"key":"2025071109313234100_ref64","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1007\/s00299-012-1285-1","article-title":"Sprout vacuum-infiltration: a simple and efficient agroinoculation method for virus-induced gene silencing in diverse solanaceous species","volume":"31","author":"Yan","year":"2012","journal-title":"Plant Cell Rep"},{"key":"2025071109313234100_ref65","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1105\/tpc.16.00922","article-title":"A multipurpose toolkit to enable advanced genome engineering in plants","volume":"29","author":"\u010cerm\u00e1k","year":"2017","journal-title":"Plant Cell"},{"key":"2025071109313234100_ref66","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.molp.2017.01.003","article-title":"CRISPR-P 2.0: an improved CRISPR-cas9 tool for genome editing in plants","volume":"10","author":"Liu","year":"2017","journal-title":"Mol Plant"},{"key":"2025071109313234100_ref67","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s11240-016-1063-9","article-title":"Modification of plant regeneration medium decreases the time for recovery of Solanum lycopersicum cultivar M82 stable transgenic lines","volume":"127","author":"Gupta","year":"2016","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"2025071109313234100_ref68","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/BF00269239","article-title":"Leaf disc transformation of cultivated tomato (L. esculentum) using agrobacterium tumefaciens","volume":"5","author":"McCormick","year":"1986","journal-title":"Plant Cell Rep"},{"key":"2025071109313234100_ref69","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1105\/tpc.12.11.2201","article-title":"A Rab1 GTPase is required for transport between the endoplasmic reticulum and golgi apparatus and for normal golgi movement in plants","volume":"12","author":"Batoko","year":"2000","journal-title":"Plant Cell"},{"key":"2025071109313234100_ref70","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.56717","article-title":"Evolution of a plant gene cluster in solanaceae and emergence of metabolic diversity","volume":"9","author":"Fan","year":"2020","journal-title":"elife"},{"key":"2025071109313234100_ref71","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/978-1-0716-1362-7_15","volume-title":"Plant Lipids","author":"Baales","year":"2021"},{"key":"2025071109313234100_ref72","doi-asserted-by":"crossref","DOI":"10.1199\/tab.0161","article-title":"Acyl-lipid metabolism","volume":"11","author":"Li-Beisson","year":"2013","journal-title":"Arabidopsis Book"},{"key":"2025071109313234100_ref73","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1186\/s12870-015-0678-z","article-title":"The development of type VI glandular trichomes in the cultivated tomato Solanum lycopersicum and a related wild species S. Habrochaites","volume":"15","author":"Bergau","year":"2015","journal-title":"BMC Plant Biol"},{"key":"2025071109313234100_ref74","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1111\/j.1365-313X.2007.03265.x","article-title":"The identification of a gene (Cwp1), silenced during Solanum evolution, which causes cuticle microfissuring and dehydration when expressed in tomato fruit","volume":"52","author":"Hovav","year":"2007","journal-title":"Plant J"},{"key":"2025071109313234100_ref75","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1194\/jlr.D001065","article-title":"Preparation of fatty acid methyl esters for gas-liquid chromatography","volume":"51","author":"Ichihara","year":"2010","journal-title":"J Lipid Res"},{"key":"2025071109313234100_ref76","doi-asserted-by":"crossref","first-page":"W29","DOI":"10.1093\/nar\/gkr367","article-title":"HMMER web server: interactive sequence similarity searching","volume":"39","author":"Finn","year":"2011","journal-title":"Nucleic Acids Res"},{"key":"2025071109313234100_ref77","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"2025071109313234100_ref78","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"2025071109313234100_ref79","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"2025071109313234100_ref80","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1038\/s41467-023-35936-z","article-title":"Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber","volume":"14","author":"Cheng","year":"2023","journal-title":"Nat Commun"}],"container-title":["Horticulture Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/hr\/advance-article-pdf\/doi\/10.1093\/hr\/uhaf114\/63019804\/uhaf114.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/hr\/article-pdf\/12\/8\/uhaf114\/63019804\/uhaf114.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/hr\/article-pdf\/12\/8\/uhaf114\/63019804\/uhaf114.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T13:31:47Z","timestamp":1752240707000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/hr\/article\/doi\/10.1093\/hr\/uhaf114\/8121008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,28]]},"references-count":80,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,7,10]]}},"URL":"https:\/\/doi.org\/10.1093\/hr\/uhaf114","relation":{},"ISSN":["2052-7276"],"issn-type":[{"value":"2052-7276","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,8]]},"published":{"date-parts":[[2025,4,28]]},"article-number":"uhaf114"}}