{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T01:18:46Z","timestamp":1772932726897,"version":"3.50.1"},"reference-count":76,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260768"],"award-info":[{"award-number":["32260768"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jiujiang Basic Research Program","award":["S2024KXJJ0001"],"award-info":[{"award-number":["S2024KXJJ0001"]}]},{"name":"Key Research Projects of Jiangxi Province","award":["20223BBG71003"],"award-info":[{"award-number":["20223BBG71003"]}]},{"name":"Talents Program of Jiangxi Province","award":["jxsq2020101088"],"award-info":[{"award-number":["jxsq2020101088"]}]},{"name":"Talents Program of Jiangxi Province","award":["jxsq2020101020"],"award-info":[{"award-number":["jxsq2020101020"]}]},{"name":"Key Program of the Chinese Academy of Sciences","award":["KJZD-SW-L13"],"award-info":[{"award-number":["KJZD-SW-L13"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Quercetin glucosides are important phytopharmaceutical metabolites in Descurainia sophia seeds, which are widely used in traditional herbal medicine. However, the key genes involved in quercetin glucoside biosynthesis in D. sophia have not been characterized. Herein, we present the telomere-to-telomere genomes of a tetraploid D. sophia, which accumulates high levels of quercetin glucoside, and a diploid D. sophia, which accumulates only trace amounts. Multiomics analyses and uridine diphosphate glucosyltransferase (UGT) enzyme assays revealed that the gene duplication and functional evolution of Dscd6AG01520, an UGT gene, led to high quercetin-3-O-\u03b2-d-glucoside and quercetin-3,7-O-\u03b2-d-diglucoside accumulation in tetraploid D. sophia seeds. Further UGT enzyme assays with the point mutations of Dscd6AG01520 showed that S213 was a critical amino acid for the enzymatic activity of Dscd6AG01520. In addition, we found that diploid D. sophia evolved from an ancestral crucifer karyotype through chromosome fusion and rearrangement. Collectively, our findings illuminate the mechanism of high quercetin glucoside accumulation in tetraploid D. sophia, clarify the origin of the diploid D. sophia genome, and provide valuable genomic resources for comparative genomics and research into polyploid evolution.<\/jats:p>","DOI":"10.1093\/hr\/uhaf335","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T08:16:38Z","timestamp":1764749798000},"source":"Crossref","is-referenced-by-count":0,"title":["Telomere-to-telomere genome assembly and multiomics analyses illustrate the high accumulation of quercetin glucosides in tetraploid\n                    <i>Descurainia sophia<\/i>"],"prefix":"10.1093","volume":"13","author":[{"given":"Weifeng","family":"Wu","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Plant Germplasm Resources Innovation and Genetic Improvement, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]},{"name":"Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianyong","family":"Wang","sequence":"additional","affiliation":[{"name":"Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]},{"name":"Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8780-8963","authenticated-orcid":false,"given":"Chengcheng","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, 100081 Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Song","sequence":"additional","affiliation":[{"name":"Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences , 518120 Shenzhen ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]},{"name":"Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]},{"name":"Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meixin","family":"Xiong","sequence":"additional","affiliation":[{"name":"Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]},{"name":"Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences , Chengdu 610061, Sichuan ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences , Chengdu 610061, Sichuan ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingbing","family":"Li","sequence":"additional","affiliation":[{"name":"Bellagen Biotechnology Co. Ltd. , Jinan 250307, Shandong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, 100081 Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Bellagen Biotechnology Co. Ltd. , Jinan 250307, Shandong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9501-1797","authenticated-orcid":false,"given":"Mingkun","family":"Huang","sequence":"additional","affiliation":[{"name":"Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8670-584X","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences , 518120 Shenzhen ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2982-9675","authenticated-orcid":false,"given":"Feng","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, 100081 Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danyu","family":"Kong","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Plant Germplasm Resources Innovation and Genetic Improvement, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]},{"name":"Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]},{"name":"Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences , Jiujiang 332900, Jiangxi ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,12,3]]},"reference":[{"key":"2026030701165651200_ref1","first-page":"45","article-title":"Descurainia sophia (L.): a weed with multiple medicinal uses. 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