{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:02:43Z","timestamp":1789466563716,"version":"build-2803163510"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan","award":["LZZ-2025-104"],"award-info":[{"award-number":["LZZ-2025-104"]}]},{"name":"International Science and Technology Cooperation Project of Jiangxi Province Fund","award":["20253BDH410003"],"award-info":[{"award-number":["20253BDH410003"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260745"],"award-info":[{"award-number":["82260745"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Andrographolide (AD) is the major bioactive component in the Chinese medicinal plant Andrographis paniculata (A. paniculata), which is widely used for its anti-inflammatory and antiviral properties. In this study, we observed that light is a critical environmental factor for AD biosynthesis and identified a potential transcription factor, ApTrihelix1, as a regulator of AD biosynthesis via RNA-seq combined with co-expression analysis. ApTrihelix1 is light-induced, and knocking down its expression downregulates AD content as well as the expression of several AD biosynthesis-related genes (ADRGs). Furthermore, through high-throughput sequencing methods (e.g. ChIP-seq) and molecular experiments (e.g. Yeast One Hybrid assay), ApTrihelix1 was found to bind to the promoter of ApCPS2 (a key ADRG) to activate its expression. Taken together, these data strongly support that light upregulates the expression of certain transcription factors (e.g. ApTrihelix1), thereby activating key ADRGs (e.g. ApCPS2) and promoting AD accumulation in A. paniculata.<\/jats:p>","DOI":"10.1093\/hr\/uhag118","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T16:07:42Z","timestamp":1775750862000},"source":"Crossref","is-referenced-by-count":2,"title":["Light-responsive transcription factor ApTrihelix1 modulates andrographolide biosynthesis via targeting\n                    <i>ApCPS2<\/i>\n                    in\n                    <i>Andrographis paniculata<\/i>"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6753-9022","authenticated-orcid":false,"given":"Ling","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences , 9 Zhiqing Road, Jiujiang, Jiangxi 332900 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences , 9 Zhiqing Road, Jiujiang, Jiangxi 332900 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Jing","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences , 9 Zhiqing Road, Jiujiang, Jiangxi 332900 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9979-0361","authenticated-orcid":false,"given":"Yufang","family":"Hu","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences , 9 Zhiqing Road, Jiujiang, Jiangxi 332900 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqing","family":"Fan","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences , 9 Zhiqing Road, Jiujiang, Jiangxi 332900 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyun","family":"Tian","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences , 9 Zhiqing Road, Jiujiang, Jiangxi 332900 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuesong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine , Guangzhou 510006 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglei","family":"Jin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine , Guangzhou 510006 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5675-0466","authenticated-orcid":false,"given":"Wei","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine , Guangzhou 510006 ,","place":["China"]},{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing 100070 ,","place":["China"]},{"name":"Key Laboratory of National Forestry and Grassland Administration for Chinese Herbal Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing 100070 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9501-1797","authenticated-orcid":false,"given":"Mingkun","family":"Huang","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences , 9 Zhiqing Road, Jiujiang, Jiangxi 332900 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,7]]},"reference":[{"key":"2026070718334132900_ref1","first-page":"1","article-title":"A critical review of Andrographis 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