{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T10:35:23Z","timestamp":1789122923763,"version":"build-2803163510"},"reference-count":76,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guizhou University Basic Research Project","award":["GDJC (2024)03"],"award-info":[{"award-number":["GDJC (2024)03"]}]},{"name":"Guizhou Provincial Major Science and Technology Projects: \u2018Analysis of Comparative Advantages in Quality of Fresh Tea Leaves in Guizhou and Construction and Application of an Evaluation System\u2019","award":["QiankeheZhongda [2025] 044"],"award-info":[{"award-number":["QiankeheZhongda [2025] 044"]}]},{"name":"Guizhou Provincial Science and Technology Projects","award":["QiankeheJichu-ZK [2022] General 111"],"award-info":[{"award-number":["QiankeheJichu-ZK [2022] General 111"]}]},{"name":"Wangmo Eight Step Tea: Integration and Demonstration of Quality and Efficiency Enhancement Technologies","award":["2021YFD1100307"],"award-info":[{"award-number":["2021YFD1100307"]}]},{"name":"Project on Guiyang Science and Technology Plan [Construction Technology Contract","award":["[2023]48-21"],"award-info":[{"award-number":["[2023]48-21"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R & D Program of China","doi-asserted-by":"crossref","award":["2021YFD1200203-1"],"award-info":[{"award-number":["2021YFD1200203-1"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Jasmonic acid (JA), a pivotal lipid-derived phytohormone, serves as a critical regulator in plant growth and defense mechanisms. However, the genetic mechanisms of OPR2 gene in JA-dependent biotic defenses of Camellia tachangensis have rarely been investigated. In this study, we performed a genome-wide association study to analyze 100,720 high-quality single nucleotide polymorphisms (SNPs) among 350 tea accessions from Guizhou province to identify genetic variations associated with JA. Analysis showed C. tachangensis displayed higher levels of JA content, further analysis identified 60 high-quality SNPs and nine candidate genes related to JA. Among them, CtOPR2 encoding 12-oxophytodienoic acid reductase 2 is responsible for catalyzing the conversion of 4,5-didehydro-JA (4,5-ddh-JA) to JA. The expression level of CtOPR2 in three tea accessions with different JA content was consistent with the dynamic changes of JA content. The expression level of synthetic (AOS, AOC, and ACX) and responsive (WRKY18 and MYC2) genes were significantly decreased and increased in asODN-CtOPR2-treated shoot tips and transgenic tobacco lines overexpressing CtOPR2, respectively, which were consistent with the JA content. These results further revealed that CtOPR2 gene played essential roles in promoting JA biosynthesis. A significant reduction in insect bite area was observed on transgenic tobacco leaves compared to wild-type leaves in feeding experiments with Spodoptera litura, highlighting that the positive regulatory function of CtOPR2 gene in JA-mediated immune responses. This study provides a robust theoretical foundation for marker-assisted selection breeding in tea, aimed at developing high-JA germplasm with potentially enhanced pest resistance for cultivation in Guizhou.<\/jats:p>","DOI":"10.1093\/hr\/uhag142","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:27:06Z","timestamp":1776770826000},"source":"Crossref","is-referenced-by-count":0,"title":["Mining of\n                    <i>CtOPR2<\/i>\n                    as an essential gene regulating jasmonic acid mediated insect tolerance in\n                    <i>Camellia tachangensis<\/i>\n                    through genome-wide association studies"],"prefix":"10.1093","volume":"13","author":[{"given":"Xingmin","family":"Zhan","sequence":"first","affiliation":[{"name":"College of Life Science\/Institute of Agro-Bioengineering, Guizhou University , Guiyang 550025 ,","place":["China"]},{"name":"The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in the Mountainous Region (Ministry of Education) , Xueshi Road, Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijing","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Animal Science, Guizhou University , Guiyang 550025 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqi","family":"Dai","sequence":"additional","affiliation":[{"name":"College of Liberal Arts and Sciences, University of Illinois at Urbana-Champaign , Urbana, IL ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjun","family":"Chen","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in the Mountainous Region (Ministry of Education) , Xueshi Road, Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlong","family":"Dai","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in the Mountainous Region (Ministry of Education) , Xueshi Road, Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinfei","family":"Song","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in the Mountainous Region (Ministry of Education) , Xueshi Road, Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science\/Institute of Agro-Bioengineering, Guizhou University , Guiyang 550025 ,","place":["China"]},{"name":"The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in the Mountainous Region (Ministry of Education) , Xueshi Road, Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzhen","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Life Science\/Institute of Agro-Bioengineering, Guizhou University , Guiyang 550025 ,","place":["China"]},{"name":"The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in the Mountainous Region (Ministry of Education) , Xueshi Road, Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"2026091105464422400_ref1","doi-asserted-by":"crossref","DOI":"10.1016\/j.gene.2019.143940","article-title":"Advances in research on functional genes of tea 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beyond","volume":"4","author":"Hong","year":"2024","journal-title":"Veg Res"},{"key":"2026091105464422400_ref6","article-title":"Genome-wide identification, molecular evolution, and expression analysis of the bHLH gene family in Mesembryanthemum crystallinum","volume":"5","author":"Zhang","year":"2025","journal-title":"Veg Res"},{"key":"2026091105464422400_ref7","article-title":"Emission pattern and anti-insect function of indole from tea plant (Camellia sinensis) attacked by tea geometrids","volume":"4","author":"Li","year":"2024","journal-title":"Bev Plant Res"},{"key":"2026091105464422400_ref8","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/1744-7917.12436","article-title":"Indirect plant defense against insect herbivores: a review","volume":"25","author":"Zainab","year":"2018","journal-title":"Insect Sci"},{"key":"2026091105464422400_ref9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev-genet-102209-163500","article-title":"New insights into 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Arabidopsis","volume":"60","author":"Shan","year":"2009","journal-title":"J Exp Bot"},{"key":"2026091105464422400_ref54","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1093\/jxb\/erw495","article-title":"Jasmonate action in plant growth and development","volume":"68","author":"Huang","year":"2017","journal-title":"J Exp Bot"},{"key":"2026091105464422400_ref55","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.mrrev.2017.05.002","article-title":"Transient and stable vector transfection: pitfalls, off-target effects, artifacts","volume":"773","author":"Stepanenko","year":"2017","journal-title":"Mutat Res Rev Mutat Res"},{"key":"2026091105464422400_ref56","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.164","article-title":"PHGD: an integrative and user-friendly database for plant hormone-related 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