{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:28:44Z","timestamp":1787923724961,"version":"build-2784847793"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the China Agriculture Research System of MOF and MARA","award":["CARS-19"],"award-info":[{"award-number":["CARS-19"]}]},{"name":"Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding-Tea Plant","award":["2021C02067-7-1"],"award-info":[{"award-number":["2021C02067-7-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Theanine, a non-protein amino acid predominantly found in tea (Camellia sinensis), is a primary contributor to the characteristic umami and sweet taste of tea infusion and is associated with numerous health benefits. The biosynthesis of its direct precursor, ethylamine, is catalyzed by the enzyme CsAlaDC. The evolutionary origin of this enzyme and the molecular basis for its functional divergence from the serine decarboxylase CsSDC, however, remain poorly understood. In this study, through comprehensive genome-wide identification, phylogenetic, structural, and domain analyses, we demonstrate that CsAlaDC originated from CsSDC via gene duplication followed by functional specialization. Phe106 and Gly168 were identified as indispensable residues governing CsAlaDC enzymatic activity in vitro and in vivo through functional validation. Crucially, we pinpointed two specific codon substitutions\u2014TAC(Tyr112) to TTT(Phe106) and TGT(Cys174) to GGT(Gly168)\u2014as key evolutionary mutations responsible for the functional shift from SDC to AlaDC activity. These findings elucidate the evolutionary trajectory of CsAlaDC and provide mechanistic insights into the molecular regulation of theanine biosynthesis in tea plants.<\/jats:p>","DOI":"10.1093\/hr\/uhag153","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T11:30:28Z","timestamp":1776166228000},"source":"Crossref","is-referenced-by-count":0,"title":["Molecular evolution and functional divergence of CsAlaDC from CsSDC reveal key determinants for theanine biosynthesis in tea plants"],"prefix":"10.1093","volume":"13","author":[{"given":"Hui","family":"Zhou","sequence":"first","affiliation":[{"name":"National Center for Tea Plant Improvement, National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peixian","family":"Bai","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Hangzhou Academy of Agricultural Science , Hangzhou 310024 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxin","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Tea Plant Improvement, National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueqi","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Tea Plant Improvement, National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liubin","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Tea Plant Improvement, National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanhua","family":"Liu","sequence":"additional","affiliation":[{"name":"National Center for Tea Plant Improvement, National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Wei","sequence":"additional","affiliation":[{"name":"National Center for Tea Plant Improvement, National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Tea Plant Improvement, National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"2026082804262380800_ref1","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1515\/znc-2009-5-614","article-title":"Ethylamine content and theanine biosynthesis in different organs of Camellia sinensis seedlings","volume":"64","author":"Deng","year":"2009","journal-title":"Z Naturforsch C J Biosci"},{"key":"2026082804262380800_ref2","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.plaphy.2009.09.009","article-title":"Distribution and biosynthesis of theanine in Theaceae plants","volume":"48","author":"Deng","year":"2010","journal-title":"Plant Physiol 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