{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:28:44Z","timestamp":1787923724920,"version":"build-2784847793"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SanNongJiuFang Science and Technology Cooperation Project of Zhejiang Province","award":["2023SNJF008"],"award-info":[{"award-number":["2023SNJF008"]}]},{"name":"Organized Scientific Research in Horticulture Discipline of Zhejiang University","award":["B231220.0005-26"],"award-info":[{"award-number":["B231220.0005-26"]}]},{"name":"Key R&D Program of Zhejiang","award":["2022C02030"],"award-info":[{"award-number":["2022C02030"]}]},{"name":"Grand Science and Technology Special Project of Zhejiang Province","award":["2021C02065"],"award-info":[{"award-number":["2021C02065"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>During the domestication of Brassica campestris (syn. Brassica rapa), significant variations in bolting time have emerged, critically impacting crop yield and quality. To elucidate the genetic basis of bolting time regulation in B. campestris, we integrated selective-sweep analysis on 365 resequenced accessions with genome-wide association studies. This identified genes including BcLMI1-LF (LATE MERISTEM IDENTITY 1), an HD-ZIP I subfamily gene in the least fractionated subgenome of B. campestris. Further, gene ontology enrichment analysis indicated that BcLMI1-LF was a key candidate regulator of bolting.\u200c Haplotype analysis demonstrated the association of Hap3 of BcLMI1-LF with the late bolting phenotype. Functional validation confirmed that BcLMI1-LF-Hap3 overexpression significantly delays bolting. Mechanistically, yeast one-hybrid (Y1H), Dual-Luciferase Reporter Assay (DLRA), and chromatin immunoprecipitation followed by quantitative polymerase chain reaction (ChIP-qPCR) assays established that BcLMI1-LF-Hap3 directly represses BcTCP4-LF (TEOSINTE BRANCHED 1\/CYCLOIDEA\/PCF 4), a conserved bolting regulator.\u200c \u200cThis study advances understanding of bolting regulation in B. campestris and provides molecular targets for breeding late-bolting crops.<\/jats:p>","DOI":"10.1093\/hr\/uhag157","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T11:41:43Z","timestamp":1776253303000},"source":"Crossref","is-referenced-by-count":0,"title":["The HD-ZIP I transcription factor BcLMI1 regulates bolting time in\n                    <i>Brassica campestris<\/i>\n                    by repressing\n                    <i>BcTCP4<\/i>"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2572-7575","authenticated-orcid":false,"given":"Jingwen","family":"Chen","sequence":"first","affiliation":[{"name":"Zhejiang University College of Agriculture and Biotechnology, Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8869-0278","authenticated-orcid":false,"given":"Yujie","family":"Wu","sequence":"additional","affiliation":[{"name":"Zhejiang University College of Agriculture and Biotechnology, Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University , Hangzhou 310058 ,","place":["China"]},{"name":"Hainan Institute of Zhejiang University , Sanya 572025 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2884-6953","authenticated-orcid":false,"given":"Airong","family":"Li","sequence":"additional","affiliation":[{"name":"Zhejiang University College of Agriculture and Biotechnology, Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0712-1165","authenticated-orcid":false,"given":"Yuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Zhejiang University College of Agriculture and Biotechnology, Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University , Hangzhou 310058 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