{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:15:11Z","timestamp":1788275711106,"version":"build-2803163510"},"reference-count":82,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Innovation Program of the Chinese Academy of Agricultural Sciences"},{"name":"Liaoning Major Science and Technology Special Project","award":["2025JH1\/11700019"],"award-info":[{"award-number":["2025JH1\/11700019"]}]},{"name":"Organization and Implementation of Crop Germplasm Resources Introduction","award":["22250268"],"award-info":[{"award-number":["22250268"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2019YFD1001301"],"award-info":[{"award-number":["2019YFD1001301"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2019YFD1001300"],"award-info":[{"award-number":["2019YFD1001300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-08"],"award-info":[{"award-number":["CARS-08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pea (Pisum sativum L.) is one of the most important edible legumes in China, with both planting area and total yield ranking among the highest in the world. Fusarium wilt, caused by Fusarium oxysporum f. sp. pisi (Fop), is a severe factor limiting pea production. The deployment of resistant pea cultivars is the most effective and sustainable strategy for controlling this disease. In the present study, a novel resistance gene PsFwC9, conferring resistance to Fop race 5, was identified in the resistant pure line Chengwan 9-8 (CW9-8), and its candidate gene Psat4g213640 was characterized and functionally validated to be associated with disease resistance. Genetic analysis of the F\u2082 population derived from the cross between the resistant parent CW9-8 and the susceptible parent Chengwan 9-1 (CW9-1) revealed that PsFwC9 was controlled by a single dominant gene. Based on whole-genome resequencing, bulked segregant analysis sequencing (BSA-seq) and fine mapping, PsFwC9 was localized to an 817.06-kb region on chromosome 4 (i.e. linkage group IV, chr4LG4), flanked by KASP markers A016508 and A016511, and co-segregated with four markers. Haplotype analysis revealed that only the marker A016615 was significantly associated with Fusarium wilt resistance, and this marker was designated as a diagnostic marker for PsFwC9. Marker A016615 was located at 425\u2009699\u2009725\u00a0bp on chr4LG4, corresponding to the 277\u00a0bp within Psat4g213640, where a \u2018A\/G\u2019 single-nucleotide polymorphism caused an amino acid substitution leading to an alteration in protein structure; therefore, Psat4g213640 was identified as the PsFwC9 candidate gene. Quantitative real-time PCR analysis showed no significant difference in the expression levels of Psat4g213640 between CW9-8 and CW9-1. Overexpression of the candidate gene Psat4g213640CW9-8 in the hairy root system significantly enhanced the resistance of CW9-1 to Fusarium wilt, whereas RNA interference-mediated silencing of Psat4g213640CW9-8 reduced the resistance of CW9-8, indicating that Psat4g213640CW9-8 played a crucial role in pea resistance to Fusarium wilt. In addition, subcellular localization showed that the protein encoded by Psat4g213640 was targeted to the endoplasmic reticulum. Collectively, these findings not only enriched the gene resources for disease resistance in pea and provided an important foundation for elucidating the molecular mechanism of PsFwC9-mediated resistance, but also provided important technical support for the practical application of molecular breeding for disease resistance in pea.<\/jats:p>","DOI":"10.1093\/hr\/uhag166","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T10:43:09Z","timestamp":1776940989000},"source":"Crossref","is-referenced-by-count":0,"title":["Identification and characterization of\n                    <i>PsFwC9<\/i>\n                    conferring Fusarium wilt resistance in pea"],"prefix":"10.1093","volume":"13","author":[{"given":"Dong","family":"Deng","sequence":"first","affiliation":[{"name":"Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing 100081 ,","place":["China"]},{"name":"Crop Research Institute, Liaoning Academy of Agricultural Sciences , Shenyang 110161 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqi","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing 100081 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Canxing","family":"Duan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing 100081 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Feng","sequence":"additional","affiliation":[{"name":"Crop Research Institute, Liaoning Academy of Agricultural Sciences , Shenyang 110161 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renfeng","family":"Xue","sequence":"additional","affiliation":[{"name":"Crop Research Institute, Liaoning Academy of Agricultural Sciences , Shenyang 110161 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weide","family":"Ge","sequence":"additional","affiliation":[{"name":"Crop Research Institute, Liaoning Academy of Agricultural Sciences , Shenyang 110161 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suli","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing 100081 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