{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:59:10Z","timestamp":1790294350386,"version":"4.1.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Natural Science Foundation of China","award":["32472735; 32272703"],"award-info":[{"award-number":["32472735; 32272703"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"the Natural Science Foundations of Anhui Province","award":["2308085QC101"],"award-info":[{"award-number":["2308085QC101"]}]},{"name":"Provincial Key Development Program for Basic Research of Anhui","award":["2023z04020005"],"award-info":[{"award-number":["2023z04020005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Trichomes, specialized epidermal cells on plant leaves, contribute to resistance against both abiotic and biotic stress. Wucai, an important leafy vegetable in China, is predominantly characterized by low trichome density or even hairlessness in commercial cultivars. However, the genetic and molecular mechanisms underlying trichome loss remain largely unknown. In this study, we investigate the genetic basis of trichome loss using a high-trichome-density wucai inbred line, W7-1, and a low-trichome-density cultivar, W7-2. Through a combination of bulked segregant analysis sequencing and map-based cloning, we identified the homolog of the Arabidopsis CPR5 (Constitutive Pathogen Response 5) gene located on chromosome A06 as the candidate locus. A 3365-bp transposon insertion in the promoter of W7-2 disrupts its transcriptional activity, leading to reduced expression of BcCPR5 and impaired trichome formation. Further analysis showed that BcCPR5 expression is positively regulated by BcWRKY44 through a W-box cis-element in the promoter. In W7-2, this transposon insertion physically separates the W-box from the transcription start site. Population genetic analysis revealed that the insertion is prevalent among low-trichome-density or hairless cultivated varieties, reflecting selection during domestication and breeding. Collectively, our findings partially uncover the genetic mechanism underlying trichome loss in wucai and establish a novel BcWRKY44\u2013BcCPR5 regulatory module controlling trichome formation.<\/jats:p>","DOI":"10.1093\/hr\/uhag184","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T11:45:37Z","timestamp":1778067937000},"source":"Crossref","is-referenced-by-count":0,"title":["A transposon insertion in the promoter of\n                    <i>BcCPR5<\/i>\n                    impairs trichome formation in wucai (\n                    <i>Brassica campestris<\/i>\n                    L.)"],"prefix":"10.1093","volume":"13","author":[{"given":"Xun","family":"Gao","sequence":"first","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangkang","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Department of Vegetable Culture and Breeding, Wanjiang Vegetable Industrial Technology Institute , Maanshan 238200 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Tang","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Department of Vegetable Culture and Breeding, Wanjiang Vegetable Industrial Technology Institute , Maanshan 238200 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Hou","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Department of Vegetable Culture and Breeding, Wanjiang Vegetable Industrial Technology Institute , Maanshan 238200 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohu","family":"Chen","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Department of Vegetable Culture and Breeding, Wanjiang Vegetable Industrial Technology Institute , Maanshan 238200 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenggang","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Department of Vegetable Culture and Breeding, Wanjiang Vegetable Industrial Technology Institute , Maanshan 238200 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Yuan","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Department of Vegetable Culture and Breeding, Wanjiang Vegetable Industrial Technology Institute , Maanshan 238200 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University , Hefei 230036 ,","place":["China"]},{"name":"Department of Vegetable Culture and Breeding, Wanjiang Vegetable Industrial Technology Institute , Maanshan 238200 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components","volume":"15","author":"Porebski","year":"1997","journal-title":"Plant Mol Biol Rep"},{"key":"2026092419120964400_ref50","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and accurate short read alignment with burrows-wheeler transform","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"2026092419120964400_ref51","article-title":"Insertion of a large retrotransposon TNT fragment in the promoter region of Brgl1 results in a gray leaf phenotype in pakchoi","volume":"5","author":"Liu","year":"2025","journal-title":"Veg Res"},{"key":"2026092419120964400_ref52","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The sequence alignment\/map format and 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