{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:59:05Z","timestamp":1790294345831,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008992","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"publisher","award":["2023A1515012007"],"award-info":[{"award-number":["2023A1515012007"]}],"id":[{"id":"10.13039\/501100008992","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32570446"],"award-info":[{"award-number":["32570446"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Epimedium sagittatum is an important medicinal plant whose main bioactive flavonol glycosides, such as epimedin C and icariin, are derived from kaempferol. However, the spatial distribution of these compounds within the plant and the genetic basis for their structural diversity remain unclear. Here, we employed MALDI-TOF mass spectrometry imaging to visualize the distribution of epimedin C and icariin in E. sagittatum leaves, revealing their predominant accumulation in nonleaf vein mesophyll part. Integrated metabolomic and transcriptomic analyses of leaves, petioles, and rhizomes highlighted glycosylation as a key contributor to kaempferol derivative diversity. We identified five candidate UDP-glycosyltransferase genes, among which UGT72B113 exhibited remarkable functional versatility. In vitro enzymatic assays demonstrated that UGT72B113 accepts multiple kaempferol-type aglycones and various sugar donors, catalyzing the formation of monoglycosylate, diglycosylate, and even triglycosylated products from a single substrate. Our findings reveal that UGT72B113 is involved in generating flavonol glycoside diversity in E. sagittatum via its broad substrate promiscuity, multi-glycosylation capability, and sugar donor flexibility. This study provides new insights into the spatial regulation of flavonol accumulation and the enzymatic mechanisms underlying phytochemical diversity in medicinal plant.<\/jats:p>","DOI":"10.1093\/hr\/uhag187","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T11:49:32Z","timestamp":1778240972000},"source":"Crossref","is-referenced-by-count":1,"title":["Spatial distribution of marker compounds in\n                    <i>Epimedium sagittatum<\/i>\n                    leaves and the role of UGT72B113 in generating kaempferol glycoside diversity"],"prefix":"10.1093","volume":"13","author":[{"given":"Bin","family":"Yan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510650 ,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghao","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510650 ,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of National Forestry and Grassland Administration on Chinese Herbal Medicine, College of Life Science, Northeast Forestry University , Harbin 150040 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanhuan","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University , Zhengzhou 450046 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxia","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510650 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxiang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510650 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510650 ,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8017-2045","authenticated-orcid":false,"given":"Yongqing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510650 ,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University , Zhengzhou 450046 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510650 ,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9578-4068","authenticated-orcid":false,"given":"Xiaoman","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, Guangdong Provincial Key Laboratory of Applied Botany, Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510650 ,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,9]]},"reference":[{"key":"2026092419120634100_ref1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.2187\/bss.17.24","article-title":"Flavonoid function and activity to plants and other 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What a dilemma for acylation in the world of plant phenolic compounds","volume":"208","author":"Bontpart","year":"2015","journal-title":"New Phytol"},{"key":"2026092419120634100_ref4","doi-asserted-by":"crossref","first-page":"655","DOI":"10.3109\/13880209.2013.853809","article-title":"A systematic review on biological activities of prenylated flavonoids","volume":"52","author":"Chen","year":"2014","journal-title":"Pharm Biol"},{"key":"2026092419120634100_ref5","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1039\/D2NP00077F","article-title":"Plant glycosyltransferases for expanding bioactive glycoside diversity","volume":"40","author":"Sirirungruang","year":"2023","journal-title":"Nat Prod Rep"},{"key":"2026092419120634100_ref6","doi-asserted-by":"crossref","first-page":"4451","DOI":"10.1007\/s11101-024-10042-0","article-title":"Flavonoid UDP-glycosyltransferase in plants: functional identification, substrate recognition mechanism, and biotechnology 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