{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T17:13:02Z","timestamp":1784740382790,"version":"3.55.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T00:00:00Z","timestamp":1648684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The hypothesis of preimplantation genetic testing for aneuploidy (PGT-A) was first proposed 20\u2009years ago, suggesting that during IVF elimination of aneuploid embryos prior to transfer will improve implantation rates of remaining embryos and, therefore, increase pregnancy and live birth rates, while also reducing miscarriages. Subsequently, unvalidated and increasingly unrestricted clinical utilization of PGT-A called for at least one properly randomized controlled trial (RCT) to assess cumulative live birth rates following a single oocyte retrieval, utilizing all fresh and frozen embryos of an IVF cycle. Only recently two such RCTs were published, however both, when properly analysed, not only failed to demonstrate significant advantages from utilization of PGT-A, but actually demonstrated outcome deficits in comparison to non-use of PGT-A, when patient selection biases in favour of PGT-A were reversed. Moreover, because of high embryo mosaicism at the blastocyst stage and, therefore, high false-positive rates from trophectoderm biopsies, large numbers of chromosomal-normal embryos with normal pregnancy potential are unnecessarily left unused or discarded, indisputably causing harm to affected couples. We, therefore, strongly call for restricting PGT-A to only research protocols and, as of this point in time, encourage professional societies in the field to follow suit with appropriate practice guidelines.<\/jats:p>","DOI":"10.1093\/humrep\/deac052","type":"journal-article","created":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T11:08:47Z","timestamp":1648465727000},"page":"2730-2734","source":"Crossref","is-referenced-by-count":36,"title":["We have reached a dead end for preimplantation genetic testing for aneuploidy"],"prefix":"10.1093","volume":"37","author":[{"given":"Norbert","family":"Gleicher","sequence":"first","affiliation":[{"name":"Center for Human Reproduction (CHR) , New York, NY, USA"},{"name":"Foundation for Reproductive Medicine , New York, NY, USA"},{"name":"Stem Cell and Embryology Laboratory, Rockefeller University , New York, NY, USA"},{"name":"Medical University of Vienna , Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7980-2369","authenticated-orcid":false,"given":"David H","family":"Barad","sequence":"additional","affiliation":[{"name":"Center for Human Reproduction (CHR) , New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pasquale","family":"Patrizio","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Miami School of Medicine , Miami, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0865-1196","authenticated-orcid":false,"given":"Raoul","family":"Orvieto","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Chaim Sheba Medical Center , Infertility and IVF Unit, Ramat Gan, Israel"},{"name":"Tel Aviv University, Sackler Medical Faculty, Tel-Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,3,31]]},"reference":[{"key":"2022120105532246500_deac052-B1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.rbmo.2018.06.018","article-title":"The emperor still looks naked","volume":"37","author":"Braude","year":"2018","journal-title":"Reprod Biomed Online"},{"key":"2022120105532246500_deac052-B2","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/j.fertnstert.2015.08.038","article-title":"Comprehensive chromosome screening improves embryo selection: a meta-analysis","volume":"104","author":"Dahdouh","year":"2015","journal-title":"Fertil Steril"},{"key":"2022120105532246500_deac052-B3","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.rbmo.2015.11.017","article-title":"The origin and significance of additional aneuploidy events in couples undergoing preimplantation genetic diagnosis for translocations by array comparative genomic hybridization","volume":"32","author":"Ghevaria","year":"2016","journal-title":"Reprod Biomed Online"},{"key":"2022120105532246500_deac052-B4","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1007\/s10815-016-0673-9","article-title":"The impact of patient preselection on reported IVF outcomes","volume":"33","author":"Gleicher","year":"2016","journal-title":"J Assist Reprod Genet"},{"key":"2022120105532246500_deac052-B5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s13048-017-0318-3","article-title":"Is the hypothesis of preimplantation genetic screening (PGS) still supportable? A review","volume":"10","author":"Gleicher","year":"2017","journal-title":"J Ovarian Res"},{"key":"2022120105532246500_deac052-B6","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.molmed.2020.11.009","article-title":"Preimplantation genetic testing for aneuploidy\u2014a castle built on sand","volume":"27","author":"Gleicher","year":"2021","journal-title":"Trends Mol Med"},{"key":"2022120105532246500_deac052-B7","doi-asserted-by":"crossref","first-page":"e9","DOI":"10.1016\/j.fertnstert.2015.07.180","article-title":"Further evidence against use of PGS in poor prognosis patients: report of normal births after transfer of embryos reported as aneuploid","volume":"104","author":"Gleicher","year":"2015","journal-title":"Fertil Steril"},{"key":"2022120105532246500_deac052-B8","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s12958-016-0193-6","article-title":"Accuracy of preimplantation genetic screening (PGS) is compromised by degree of mosaicism of human embryos","volume":"14","author":"Gleicher","year":"2016","journal-title":"Reprod Biol Endocrinol"},{"key":"2022120105532246500_deac052-B9","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.1056\/NEJMc1500421","article-title":"Healthy babies after intrauterine transfer of mosaic aneuploid blastocysts","volume":"373","author":"Greco","year":"2015","journal-title":"N Engl J Med"},{"key":"2022120105532246500_deac052-B10","first-page":"312","article-title":"Human embryos commonly form abnormal nuclei during developmentL a mechanism of DNA damage, embryonic aneuploidy, and developmental arrest","volume":"31","author":"Kort","year":"2016","journal-title":"Hum Reprod"},{"key":"2022120105532246500_deac052-B11","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.1056\/NEJMsb2101718","article-title":"The imperative f responsible innovation in reproductive medicine","volume":"385","author":"Mastenbroek","year":"2021","journal-title":"N Engl J Med"},{"key":"2022120105532246500_deac052-B12","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1093\/humupd\/dmr003","article-title":"Preimplantation genetic screening: a systematic review and meta-analysis","volume":"17","author":"Mastenbroek","year":"2011","journal-title":"Hum Reprod Update"},{"key":"2022120105532246500_deac052-B13","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1016\/j.fertnstert.2016.08.017","article-title":"Why do euploid embryos miscarry? A case-control study comparing the rate of aneuploidy within presumed euploid embryos that resulted in miscarriage or live birth using next-generation sequencing","volume":"106","author":"Maxwell","year":"2016","journal-title":"Fertil Steril"},{"issue":"Suppl 1","key":"2022120105532246500_deac052-B14","first-page":"i14","article-title":"Embryos showing mosaicism in trophectoderm cells can achieve good pregnancy rates","volume":"31","author":"Morales","year":"2016","journal-title":"Hum Reprod"},{"key":"2022120105532246500_deac052-B15","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/j.fertnstert.2019.07.1346","article-title":"Preimplantation genetic testing for aneuploidy versus morphology as selection criteria for single frozen-thawed embryo transfer in good-prognosis patients: a multicenter randomized clinical trial","volume":"112","author":"Munn\u00e9","year":"2019","journal-title":"Fertil Steril"},{"key":"2022120105532246500_deac052-B16","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.fertnstert.2016.11.013","article-title":"High relative deoxyribonucleic acid content of trophec-toderm biopsy adversely afects pregnancy outcomes","volume":"107","author":"Neal","year":"2017","journal-title":"Fertil Steril"},{"key":"2022120105532246500_deac052-B17","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s12958-016-0171-z","article-title":"Preimplantation genetic screening\u2014the required RCT that has not yet been carried out","volume":"14","author":"Orvieto","year":"2016","journal-title":"Reprod Biol Endocrinol"},{"key":"2022120105532246500_deac052-B18","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ejogrb.2020.07.052","article-title":"The reproducibility of trophectoderm biopsies\u2014the chaos behind preimplantation genetic testing for aneuploidy","volume":"254","author":"Orvieto","year":"2020","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"2022120105532246500_deac052-B19","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1007\/s10815-020-01705-w","article-title":"Preimplantation genetic testing for aneuploidy (PGT-A)\u2014finally revealed","volume":"37","author":"Orvieto","year":"2020","journal-title":"J Assist Reprod Genet"},{"key":"2022120105532246500_deac052-B20","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1007\/s10815-016-0801-6","article-title":"Should preimplantation genetic screening (PGS) be implemented to routine IVF practice?","volume":"33","author":"Orvieto","year":"2016","journal-title":"J Assist Reprod Genet"},{"key":"2022120105532246500_deac052-B21","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s12958-020-00650-8","article-title":"Do human embryos have the ability of self-correction?","volume":"18","author":"Orvieto","year":"2020","journal-title":"Reprod Biol Endocrinol"},{"key":"2022120105532246500_deac052-B22","doi-asserted-by":"crossref","first-page":"506","DOI":"10.3109\/09513590.2016.1142962","article-title":"Should pre-implantation genetic screening be implemented to routine clinical practice?","volume":"32","author":"Orvieto","year":"2016","journal-title":"Gynecol Endocrinol"},{"key":"2022120105532246500_deac052-B23","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1007\/s10815-019-01510-0","article-title":"Worldwide live births following transfer of chromosomally \u201cabnormal\u201d embryos after PGT\/A. 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