{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:58:13Z","timestamp":1787169493143,"version":"build-2736575974"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["CRU326"],"award-info":[{"award-number":["CRU326"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The amount of single-cell RNA-sequencing (scRNA-seq) data produced in the field of human male reproduction has steadily increased. Transcriptional profiles of thousands of testicular cells have been generated covering the human neonatal, prepubertal, pubertal and adult period as well as different types of male infertility; the latter include non-obstructive azoospermia, cryptozoospermia, Klinefelter syndrome and azoospermia factor deletions. In this review, we provide an overview of transcriptional changes in different testicular subpopulations during postnatal development and in cases of male infertility. Moreover, we review novel concepts regarding the existence of spermatogonial and somatic cell subtypes as well as their crosstalk and provide corresponding marker genes to facilitate their identification. We discuss the potential clinical implications of scRNA-seq findings, the need for spatial information and the necessity to corroborate findings by exploring other levels of regulation, including at the epigenetic or protein level.<\/jats:p>","DOI":"10.1093\/humrep\/deac245","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T10:31:52Z","timestamp":1668681112000},"page":"1-13","source":"Crossref","is-referenced-by-count":50,"title":["Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9279-7373","authenticated-orcid":false,"given":"Sara","family":"Di Persio","sequence":"first","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology, University Hospital of M\u00fcnster , M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0181-6194","authenticated-orcid":false,"given":"Nina","family":"Neuhaus","sequence":"additional","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology, University Hospital of M\u00fcnster , M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,11,21]]},"reference":[{"key":"2023010801273462700_deac245-B1","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1111\/j.1365-2605.2011.01225.x","article-title":"High levels of the extracellular matrix proteoglycan decorin are associated with inhibition of testicular function: decorin and testicular function","volume":"35","author":"Adam","year":"2012","journal-title":"Int J Androl"},{"key":"2023010801273462700_deac245-B2","doi-asserted-by":"crossref","first-page":"5205","DOI":"10.1038\/s41467-021-25544-0","article-title":"Aging, inflammation 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