{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:24:28Z","timestamp":1790166268631,"version":"4.1.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T00:00:00Z","timestamp":1695340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["P30-ES019776"],"award-info":[{"award-number":["P30-ES019776"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01-ES009718"],"award-info":[{"award-number":["R01-ES009718"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01-ES022955"],"award-info":[{"award-number":["R01-ES022955"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["P30-ES000002"],"award-info":[{"award-number":["P30-ES000002"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R00-ES026648"],"award-info":[{"award-number":["R00-ES026648"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["T32-ES012870"],"award-info":[{"award-number":["T32-ES012870"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>STUDY QUESTION<\/jats:title>\n                    <jats:p>What metabolic pathways and metabolites in the serum and follicular fluid are associated with peak estradiol levels and the number of mature oocytes?<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>SUMMARY ANSWER<\/jats:title>\n                    <jats:p>In the serum metabolome, mostly fatty acid and amino acid pathways were associated with estradiol levels and mature oocytes while in the follicular fluid metabolome, mostly lipid, vitamin, and hormone pathways were associated with peak estradiol levels and mature oocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>WHAT IS KNOWN ALREADY<\/jats:title>\n                    <jats:p>Metabolomics has identified several metabolic pathways and metabolites associated with infertility but limited data are available for ovarian stimulation outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN, SIZE, DURATION<\/jats:title>\n                    <jats:p>A prospective cohort study of women undergoing IVF from 2009 to 2015.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>PARTICIPANTS\/MATERIALS, SETTING, METHODS<\/jats:title>\n                    <jats:p>A total of 125 women undergoing a fresh IVF cycle at a fertility clinic in the Northeast United States who provided a serum and follicular fluid sample. Untargeted metabolomics profiling was conducted using liquid chromatography with high-resolution mass spectrometry in two chromatography columns (C18 and hydrophilic interaction chromatography (HILIC)). The main ovarian stimulation outcomes were peak serum estradiol levels and number of mature oocytes. We utilized adjusted generalized linear regression models to identify significant metabolic features. Models were adjusted for age,BMI, initial infertility diagnosis, and ovarian stimulation protocol. We then conducted pathway analysis using mummichog and metabolite annotation using level-1 evidence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>MAIN RESULTS AND ROLE OF CHANCE<\/jats:title>\n                    <jats:p>In the serum metabolome, 480 and 850 features were associated with peak estradiol levels in the C18 and HILIC columns, respectively. Additionally, 437 and 538 features were associated with mature oocytes in the C18 and HILIC columns, respectively. In the follicular fluid metabolome, 752 and 929 features were associated with peak estradiol levels in the C18 and HILIC columns, respectively, Additionally, 993 and 986 features were associated with mature oocytes in the C18 and HILIC columns, respectively. The most common pathways associated with peak estradiol included fatty acids (serum and follicular fluid), hormone (follicular fluid), and lipid pathways (follicular fluid). The most common pathways associated with the number of mature oocytes retrieved included amino acids (serum), fatty acids (serum and follicular fluid), hormone (follicular fluid), and vitamin pathways(follicular fluid). The vitamin D3 pathway had the strongest association with both ovarian stimulation outcomes in the follicularfluid. Four and nine metabolites were identified using level-1 evidence (validated identification) in the serum and follicular fluid metabolomes, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>LIMITATIONS, REASONS FOR CAUTION<\/jats:title>\n                    <jats:p>Our sample was majority White and highly educated and may not be generalizable to thewider population. Additionally, residual confounding is possible and the flushing medium used in the follicular fluid could have diluted our results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>WIDER IMPLICATIONS OF THE FINDINGS<\/jats:title>\n                    <jats:p>The pathways and metabolites identified by our study provide novel insights into the biologicalmechanisms in the serum and follicular fluid that may underlie follicular and oocyte development, which could potentially be used to improve ovarian stimulation outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY FUNDING\/COMPETING INTEREST(S)<\/jats:title>\n                    <jats:p>This work was supported by the following grants from the National Institute of Environmental Health Sciences (P30-ES019776, R01-ES009718, R01-ES022955, P30-ES000002, R00-ES026648, and T32-ES012870), and National Institute of Diabetes and Digestive and Kidney Diseases\u00a0(P30DK046200). The authors have no competing interests to disclose.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>TRIAL REGISTRATION NUMBER<\/jats:title>\n                    <jats:p>N\/A.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/humrep\/dead189","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T16:33:15Z","timestamp":1694449995000},"page":"2196-2207","source":"Crossref","is-referenced-by-count":22,"title":["Serum and follicular fluid metabolome and markers of ovarian stimulation"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0078-301X","authenticated-orcid":false,"given":"Robert B","family":"Hood","sequence":"first","affiliation":[{"name":"Department of Epidemiology, Emory University Rollins School of Public Health , Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghai","family":"Liang","sequence":"additional","affiliation":[{"name":"Gangarosa Department of Environmental Health, Emory University Rollins School of Public Health , Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youran","family":"Tan","sequence":"additional","affiliation":[{"name":"Gangarosa Department of Environmental Health, Emory University Rollins School of Public Health , Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer B","family":"Ford","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, Harvard T.H. Chan School of Public Health , Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Souter","sequence":"additional","affiliation":[{"name":"Vincent Obstetrics and Gynecology, Massachusetts General Hospital and Harvard Medical School , Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4436-9630","authenticated-orcid":false,"given":"Jorge E","family":"Chavarro","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Harvard T.H. Chan School of Public Health , Boston, MA, USA"},{"name":"Department of Nutrition, Harvard T.H. Chan School of Public Health , Boston, USA"},{"name":"Channing Division of Network Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School , Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean P","family":"Jones","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy, & Critical Care Medicine, Emory University School of Medicine , Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russ","family":"Hauser","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, Harvard T.H. Chan School of Public Health , Boston, MA, USA"},{"name":"Department of Epidemiology, Harvard T.H. Chan School of Public Health , Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0812-7439","authenticated-orcid":false,"given":"Audrey J","family":"Gaskins","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Emory University Rollins School of Public Health , Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,9,22]]},"reference":[{"key":"2023110708023650000_dead189-B1","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.rbmo.2017.12.010","article-title":"Lower follicular fluid vitamin D concentration is related to a higher number of large ovarian follicles","volume":"36","author":"Antunes","year":"2018","journal-title":"Reprod Biomed Online"},{"key":"2023110708023650000_dead189-B2","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1093\/humupd\/dmq039","article-title":"Understanding subfertility at a molecular level in the female through the application of nuclear magnetic resonance (NMR) spectroscopy","volume":"17","author":"Baskind","year":"2011","journal-title":"Hum Reprod Update"},{"key":"2023110708023650000_dead189-B3","first-page":"323","article-title":"Serum metabolites as predictive molecular markers of ovarian response to controlled stimulation: a pilot study","volume":"23","author":"Borges","year":"2019","journal-title":"JBRA Assist Reprod"},{"key":"2023110708023650000_dead189-B4","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s11306-019-1481-x","article-title":"NMR metabolic profiling of follicular fluid for investigating the different causes of female infertility: a pilot study","volume":"15","author":"Castiglione Morelli","year":"2019","journal-title":"Metabolomics"},{"key":"2023110708023650000_dead189-B5","author":"CDC","year":"2021"},{"key":"2023110708023650000_dead189-B6","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1038\/nbt.2377","article-title":"A cross-platform toolkit for mass spectrometry and proteomics","volume":"30","author":"Chambers","year":"2012","journal-title":"Nat Biotechnol"},{"key":"2023110708023650000_dead189-B7","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1007\/s10815-018-1179-4","article-title":"Vitamin D as a follicular marker of human oocyte quality and a serum marker of invitro fertilization outcome","volume":"35","author":"Ciepiela","year":"2018","journal-title":"J Assist Reprod Genet"},{"key":"2023110708023650000_dead189-B8","doi-asserted-by":"crossref","first-page":"385","DOI":"10.3390\/nu3040385","article-title":"Vitamin A in reproduction and development","volume":"3","author":"Clagett-Dame","year":"2011","journal-title":"Nutrients"},{"key":"2023110708023650000_dead189-B9","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s11306-018-1350-z","article-title":"Hyper response to ovarian stimulation affects the follicular fluid metabolomic profile of women undergoing IVF similarly to polycystic ovary syndrome","volume":"14","author":"Cordeiro","year":"2018","journal-title":"Metabolomics"},{"key":"2023110708023650000_dead189-B10","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1093\/humrep\/dex303","article-title":"Targeted metabolomics reveals reduced levels of polyunsaturated choline plasmalogens and a smaller dimethylarginine\/arginine ratio in the follicular fluid of patients with a diminished ovarian reserve","volume":"32","author":"de la Barca","year":"2017","journal-title":"Hum Reprod"},{"key":"2023110708023650000_dead189-B11","first-page":"1","article-title":"Poor ovarian response and the possible role of natural and modified natural cycles","volume":"16","author":"Di Guardo","year":"2022","journal-title":"Ther Adv Reprod Health"},{"key":"2023110708023650000_dead189-B12","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s10815-020-01693-x","article-title":"High-resolution (1)h-nmr spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age","volume":"37","author":"Dogan","year":"2020","journal-title":"J Assist Reprod Genet"},{"key":"2023110708023650000_dead189-B13","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.fertnstert.2014.11.015","article-title":"Oocyte environment: follicular fluid and cumulus cells are critical for oocyte health","volume":"103","author":"Dumesic","year":"2015","journal-title":"Fertil Steril"},{"key":"2023110708023650000_dead189-B14","doi-asserted-by":"crossref","first-page":"77002","DOI":"10.1289\/EHP4601","article-title":"Time-varying exposure to air pollution and outcomes of invitro fertilization among couples from a fertility clinic","volume":"127","author":"Gaskins","year":"2019","journal-title":"Environ Health Perspect"},{"key":"2023110708023650000_dead189-B15","doi-asserted-by":"crossref","first-page":"106666","DOI":"10.1016\/j.envint.2021.106666","article-title":"Periconception air pollution, metabolomic biomarkers, and fertility among women undergoing assisted reproduction","volume":"155","author":"Gaskins","year":"2021","journal-title":"Environ Int"},{"key":"2023110708023650000_dead189-B16","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1093\/toxsci\/kfv198","article-title":"Reference standardization for mass spectrometry and high-resolution metabolomics applications to exposome research","volume":"148","author":"Go","year":"2015","journal-title":"Toxicol Sci"},{"key":"2023110708023650000_dead189-B17","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/s12958-022-00938-x","article-title":"Effects of nicotinamide on follicular development and the quality of oocytes","volume":"20","author":"Guo","year":"2022","journal-title":"Reprod Biol Endocrinol"},{"key":"2023110708023650000_dead189-B18","doi-asserted-by":"crossref","first-page":"107061","DOI":"10.1016\/j.envint.2021.107061","article-title":"Pesticide residue intake from fruits and vegetables and alterations in the serum metabolome of women undergoing infertility treatment","volume":"160","author":"Hood","year":"2022","journal-title":"Environ Int"},{"key":"2023110708023650000_dead189-B19","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/j.fertnstert.2022.07.023","article-title":"Characterizing the follicular fluid metabolome: quantifying the correlation across follicles and differences with the serum metabolome","volume":"118","author":"Hood","year":"2022","journal-title":"Fertil Steril"},{"key":"2023110708023650000_dead189-B20","doi-asserted-by":"crossref","first-page":"e191","DOI":"10.1097\/EE9.0000000000000191","article-title":"Length of pm(2.5) exposure and alterations in the serum metabolome among women undergoing infertility treatment","volume":"6","author":"Hood","year":"2022","journal-title":"Environ Epidemiol"},{"key":"2023110708023650000_dead189-B21","doi-asserted-by":"crossref","first-page":"818888","DOI":"10.3389\/fendo.2022.818888","article-title":"Age-dependent metabolomic profile of the follicular fluids from women undergoing assisted reproductive technology treatment","volume":"13","author":"Huang","year":"2022","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2023110708023650000_dead189-B22","doi-asserted-by":"crossref","first-page":"107552","DOI":"10.1016\/j.envint.2022.107552","article-title":"Using follicular fluid metabolomics to investigate the association between air pollution and oocyte quality","volume":"169","author":"Hwang","year":"2022","journal-title":"Environ Int"},{"key":"2023110708023650000_dead189-B23","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1055\/s-2007-1002082","article-title":"Distribution of free amino acids in human preovulatory follicles","volume":"25","author":"Jimena","year":"1993","journal-title":"Horm Metab Res"},{"key":"2023110708023650000_dead189-B24","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.1016\/j.fertnstert.2011.01.154","article-title":"Associations between free fatty acids, cumulus oocyte complex morphology and ovarian function during invitro fertilization","volume":"95","author":"Jungheim","year":"2011","journal-title":"Fertil Steril"},{"key":"2023110708023650000_dead189-B25","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1093\/humrep\/des216","article-title":"A decrease in serum estradiol levels after human chorionic gonadotrophin administration predicts significantly lower clinical pregnancy and live birth rates in invitro fertilization cycles","volume":"27","author":"Kondapalli","year":"2012","journal-title":"Hum Reprod"},{"key":"2023110708023650000_dead189-B26","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1530\/EJE-11-0984","article-title":"Vitamin D and fertility: a systematic review","volume":"166","author":"Lerchbaum","year":"2012","journal-title":"Eur J Endocrinol"},{"key":"2023110708023650000_dead189-B27","doi-asserted-by":"crossref","first-page":"e1003123","DOI":"10.1371\/journal.pcbi.1003123","article-title":"Predicting network activity from high throughput metabolomics","volume":"9","author":"Li","year":"2013","journal-title":"PLoS Comput Biol"},{"key":"2023110708023650000_dead189-B28","doi-asserted-by":"crossref","first-page":"110506","DOI":"10.1016\/j.envres.2020.110506","article-title":"Application of high-resolution metabolomics to identify biological pathways perturbed by traffic-related air pollution","volume":"193","author":"Li","year":"2021","journal-title":"Environ Res"},{"key":"2023110708023650000_dead189-B29","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.envint.2019.04.003","article-title":"Perturbations of the arginine metabolome following exposures to traffic-related air pollution in a panel of commuters with and without asthma","volume":"127","author":"Liang","year":"2019","journal-title":"Environ Int"},{"key":"2023110708023650000_dead189-B30","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.envint.2018.07.044","article-title":"Use of high-resolution metabolomics for the identification of metabolic signals associated with traffic-related air pollution","volume":"120","author":"Liang","year":"2018","journal-title":"Environ Int"},{"key":"2023110708023650000_dead189-B31","doi-asserted-by":"crossref","first-page":"8836","DOI":"10.1021\/acs.analchem.0c00338","article-title":"Reference standardization for quantification and harmonization of large-scale metabolomics","volume":"92","author":"Liu","year":"2020","journal-title":"Anal Chem"},{"key":"2023110708023650000_dead189-B32","first-page":"130","article-title":"Oral vitamin D supplementation impacts gene expression in granulosa cells in women undergoing IVF","volume":"36","author":"Makieva","year":"2021","journal-title":"Hum Reprod"},{"key":"2023110708023650000_dead189-B33","author":"Mathews","year":"2016"},{"key":"2023110708023650000_dead189-B34","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/s10815-019-01670-z","article-title":"The lipidome of endometrial fluid differs between implantative and non-implantative IVF cycles","volume":"37","author":"Matorras","year":"2020","journal-title":"J Assist Reprod Genet"},{"key":"2023110708023650000_dead189-B35","doi-asserted-by":"crossref","first-page":"603167","DOI":"10.1155\/2013\/603167","article-title":"Metabolite profiling in the pursuit of biomarkers for IVF outcome: the case for metabolomics studies","volume":"2013","author":"McRae","year":"2013","journal-title":"Int J Reprod Med"},{"key":"2023110708023650000_dead189-B36","doi-asserted-by":"crossref","DOI":"10.1093\/hropen\/hoy001","article-title":"The environment and reproductive health (earth) study: a prospective preconception cohort","author":"Messerlian","year":"2018","journal-title":"Hum Reprod Open"},{"key":"2023110708023650000_dead189-B37","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1007\/s10815-019-01428-7","article-title":"Understanding mechanisms of oocyte development by follicular fluid lipidomics","volume":"36","author":"Montani","year":"2019","journal-title":"J Assist Reprod Genet"},{"key":"2023110708023650000_dead189-B38","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1530\/REP-13-0184","article-title":"Metabolic profiling of human follicular fluid identifies potential biomarkers of oocyte developmental competence","volume":"146","author":"O'Gorman","year":"2013","journal-title":"Reproduction"},{"key":"2023110708023650000_dead189-B39","doi-asserted-by":"crossref","first-page":"11725","DOI":"10.1021\/ac402503m","article-title":"Metabolite profiling of a NIST standard reference material for human plasma (SRM 1950): GC-MS, LC-MS, NMR, and clinical laboratory analyses, libraries, and web-based resources","volume":"85","author":"Sim\u00f3n-Manso","year":"2013","journal-title":"Anal Chem"},{"key":"2023110708023650000_dead189-B40","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/S1472-6483(10)60153-8","article-title":"Amino acid and fatty acid composition of follicular fluid as predictors of in-vitro embryo development","volume":"16","author":"Sinclair","year":"2008","journal-title":"Reprod Biomed Online"},{"key":"2023110708023650000_dead189-B41","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1007\/978-1-4614-4711-5_9","article-title":"The blood-follicle barrier (BFB) in disease and in ovarian function","volume":"763","author":"Siu","year":"2012","journal-title":"Adv Exp Med Biol"},{"key":"2023110708023650000_dead189-B42","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1080\/19396368.2020.1718244","article-title":"Follicular fat-soluble vitamins as markers of oocyte competency","volume":"66","author":"Skowro\u0144ska","year":"2020","journal-title":"Syst Biol Reprod Med"},{"key":"2023110708023650000_dead189-B43","doi-asserted-by":"crossref","first-page":"774667","DOI":"10.3389\/fendo.2021.774667","article-title":"Metabolomic profiling of poor ovarian response identifies potential predictive biomarkers","volume":"12","author":"Song","year":"2021","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2023110708023650000_dead189-B44","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s11306-007-0082-2","article-title":"Proposed minimum reporting standards for chemical analysis chemical analysis working group (CAWG) metabolomics standards initiative (MSI)","volume":"3","author":"Sumner","year":"2007","journal-title":"Metabolomics"},{"key":"2023110708023650000_dead189-B45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.ss7104a1","article-title":"Assisted reproductive technology surveillance\u2014United States, 2018","volume":"71","author":"Sunderam","year":"2022","journal-title":"MMWR Surveill Summ"},{"key":"2023110708023650000_dead189-B46","first-page":"105","article-title":"Side effects of ovarian hyperstimulation: hormonal and lipid profile changes","volume":"39","author":"Tadmor","year":"1994","journal-title":"Int J Fertil Menopausal Stud"},{"key":"2023110708023650000_dead189-B47","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/humupd\/8.2.141","article-title":"The variability of female reproductive ageing","volume":"8","author":"te Velde","year":"2002","journal-title":"Hum Reprod Update"},{"key":"2023110708023650000_dead189-B48","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1016\/j.fertnstert.2012.11.037","article-title":"Prevalence of infertility in the United States as estimated by the current duration approach and a traditional constructed approach","volume":"99","author":"Thoma","year":"2013","journal-title":"Fertil Steril"},{"key":"2023110708023650000_dead189-B49","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1471-2105-14-15","article-title":"Xmsanalyzer: Automated pipeline for improved feature detection and downstream analysis of large-scale, non-targeted metabolomics data","volume":"14","author":"Uppal","year":"2013","journal-title":"BMC Bioinformatics"},{"key":"2023110708023650000_dead189-B50","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1097\/GCO.0000000000000452","article-title":"What is new in the management of poor ovarian response in IVF?","volume":"30","author":"Vaiarelli","year":"2018","journal-title":"Curr Opin Obstet Gynecol"},{"key":"2023110708023650000_dead189-B51","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/s12884-023-05438-3","article-title":"Effect of the ratios of estradiol increase on the outcome of invitro fertilization-embryo transfer with antagonist regimens: a single center retrospective cohort study","volume":"23","author":"Wei","year":"2023","journal-title":"BMC Pregnancy Childbirth"},{"key":"2023110708023650000_dead189-B52","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/1477-7827-8-117","article-title":"Estradiol, progesterone, testosterone profiles in human follicular fluid and cultured granulosa cells from luteinized pre-ovulatory follicles","volume":"8","author":"Wen","year":"2010","journal-title":"Reprod Biol Endocrinol"},{"key":"2023110708023650000_dead189-B53","doi-asserted-by":"crossref","first-page":"3357","DOI":"10.12659\/MSM.906602","article-title":"Lipidomic components alterations of human follicular fluid reveal the relevance of improving clinical outcomes in women using progestin-primed ovarian stimulation compared to short-term protocol","volume":"24","author":"Wen","year":"2018","journal-title":"Med Sci Monit"},{"key":"2023110708023650000_dead189-B54","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.cca.2016.03.001","article-title":"A fatty acid profiling method using liquid chromatography-high resolution mass spectrometry for improvement of assisted reproductive technology","volume":"456","author":"Yagi","year":"2016","journal-title":"Clin Chim Acta"},{"key":"2023110708023650000_dead189-B55","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/s42003-022-03710-4","article-title":"Metabolic signatures in human follicular fluid identify lysophosphatidylcholine as a predictor of follicular development","volume":"5","author":"Yang","year":"2022","journal-title":"Commun Biol"},{"key":"2023110708023650000_dead189-B56","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.rbmo.2021.11.012","article-title":"Amino acid signatures in relation to polycystic ovary syndrome and increased risk of different metabolic disturbances","volume":"44","author":"Ye","year":"2022","journal-title":"Reprod Biomed Online"},{"key":"2023110708023650000_dead189-B57","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1007\/s10815-012-9831-x","article-title":"The relationship between pregnancy and oxidative stress markers on patients undergoing ovarian stimulations","volume":"29","author":"Younis","year":"2012","journal-title":"J Assist Reprod Genet"},{"key":"2023110708023650000_dead189-B58","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1093\/bioinformatics\/btp291","article-title":"apLCMS-adaptive processing of high-resolution LC\/MS Data","volume":"25","author":"Yu","year":"2009","journal-title":"Bioinformatics"},{"key":"2023110708023650000_dead189-B59","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.rbmo.2020.09.024","article-title":"Fatty acids of follicular fluid phospholipids and triglycerides display distinct association with IVF outcomes","volume":"42","author":"Zarezadeh","year":"2021","journal-title":"Reprod Biomed Online"},{"key":"2023110708023650000_dead189-B60","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s12958-020-00599-8","article-title":"Study on follicular fluid metabolomics components at different ages based on lipid metabolism","volume":"18","author":"Zhang","year":"2020","journal-title":"Reprod Biol Endocrinol"}],"container-title":["Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/humrep\/article-pdf\/38\/11\/2196\/52727000\/dead189.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/humrep\/article-pdf\/38\/11\/2196\/52727000\/dead189.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T03:03:59Z","timestamp":1699326239000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/humrep\/article\/38\/11\/2196\/7281034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,22]]},"references-count":60,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,9,22]]},"published-print":{"date-parts":[[2023,11,2]]}},"URL":"https:\/\/doi.org\/10.1093\/humrep\/dead189","relation":{},"ISSN":["0268-1161","1460-2350"],"issn-type":[{"value":"0268-1161","type":"print"},{"value":"1460-2350","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,11,1]]},"published":{"date-parts":[[2023,9,22]]}}}