{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T17:17:44Z","timestamp":1793035064592,"version":"4.5.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2703000"],"award-info":[{"award-number":["2022YFC2703000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20662"],"award-info":[{"award-number":["U24A20662"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271651"],"award-info":[{"award-number":["82271651"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Clinical Research Fund of Guangdong Medical Association","award":["2025SZ-B1001"],"award-info":[{"award-number":["2025SZ-B1001"]}]},{"name":"Postdoctoral Fellowship Program of CPSF","award":["GZC20233216"],"award-info":[{"award-number":["GZC20233216"]}]},{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"publisher","award":["2023A1515110325"],"award-info":[{"award-number":["2023A1515110325"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>STUDY QUESTION<\/jats:title>\n                    <jats:p>How does iron overload affect ovarian function in pediatric patients with transfusion-dependent beta-thalassemia major?<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>SUMMARY ANSWER<\/jats:title>\n                    <jats:p>Iron overload in pediatric patients with beta-thalassemia major is strongly associated with decreased ovarian reserve and mitochondrial damage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>WHAT IS KNOWN ALREADY<\/jats:title>\n                    <jats:p>Beta-thalassemia major is a severe blood disorder necessitating frequent blood transfusions, leading to iron overload. Excessive iron causes oxidative stress and damage in various organs, including the ovaries, but its impact on ovarian function in pediatric patients has not been fully explored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN, SIZE, DURATION<\/jats:title>\n                    <jats:p>This study analyzed data from 194 pediatric patients who were treated at The Sixth Affiliated Hospital of Sun Yat-Sen University between January 2021 and January 2024, among whom 138 had beta-thalassemia major. Meanwhile, we conducted experiments using human samples and mouse models of iron overload.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>PARTICIPANTS\/MATERIALS, SETTING, METHODS<\/jats:title>\n                    <jats:p>The study involved 194 pediatric patients. Clinical assessments measured serum ferritin and ovarian reserve indicators anti-M\u00fcllerian hormone (AMH) and antral follicle count. Ovarian tissue from pediatric patients was used for histopathological examination, immunohistochemical (IHC) staining, transmission electron microscopy (TEM), and transcriptome analysis, to investigate the effects of iron overload. For the animal experiments, we utilized 20 newborn female mice to establish an iron overload and control model. These animals underwent comprehensive evaluations, including histopathological examination, TEM, and IHC analyses, to systematically investigate the impact of iron overload on ovarian tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>MAIN RESULTS AND THE ROLE OF CHANCE<\/jats:title>\n                    <jats:p>Pubertal pediatric patients with high iron overload had significantly lower AMH, indicating reduced ovarian reserve, accompanied by evident mitochondrial damage and fibrotic remodeling in ovarian tissues, collectively contributing to impaired ovarian function. Decreased AMH and impaired follicular development were also observed in iron-overloaded mice, along with mitochondrial damage and ovarian fibrosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>LARGE SCALE DATA<\/jats:title>\n                    <jats:p>Transcriptome data are available at China National Center for Bioinformation, reference HRA004373 and HRA013348.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>LIMITATIONS, REASONS FOR CAUTION<\/jats:title>\n                    <jats:p>The cross-sectional design limits causality assessment. The sample size for molecular analyses is relatively small, necessitating larger, longitudinal studies to confirm these findings and understand long-term effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>WIDER IMPLICATIONS OF THE FINDINGS<\/jats:title>\n                    <jats:p>Effective iron management in pediatric patients with beta-thalassemia major is crucial to preserving ovarian function and preventing long-term reproductive issues. These findings can guide clinical practices and therapeutic strategies to mitigate iron overload\u2019s adverse effects on ovarian health. In this study, mitochondrial dysfunction may compromise cellular energy production and amplify tissue vulnerability to ischemia-reperfusion injury during transplantation. Additionally, fibrosis-induced structural rigidity may hinder revascularization of transplanted tissue, further reducing success rates. These findings underscore the need to monitor post-transplant survival rates. Further experimental investigations are warranted to explore this critical issue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY FUNDING\/COMPETING INTEREST(S)<\/jats:title>\n                    <jats:p>This work was supported by the National Key R&amp;D Program of China (2022YFC2703000); National Natural Science Foundation of China (U24A20662, 82271651); Clinical Research Fund of Guangdong Medical Association (2025SZ-B1001); Postdoctoral Fellowship Program of CPSF (GZC20233216); and Guangdong Basic and Applied Basic Research Foundation (2023A1515110325). The authors declared no competing interests.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/humrep\/deaf250","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T13:09:54Z","timestamp":1765458594000},"page":"410-426","source":"Crossref","is-referenced-by-count":2,"title":["Ovarian function compromise in pediatric beta-thalassemia major: a consequence of iron overload-induced mitochondrial and fibrotic damage"],"prefix":"10.1093","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5054-9817","authenticated-orcid":false,"given":"Xiaoping","family":"Liu","sequence":"first","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0843-7739","authenticated-orcid":false,"given":"Peigen","family":"Chen","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfang","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weie","family":"Zhao","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoke","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Sun","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchun","family":"Guo","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8835-0694","authenticated-orcid":false,"given":"Cong","family":"Fang","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9701-3865","authenticated-orcid":false,"given":"Xiaoyan","family":"Liang","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2847-4184","authenticated-orcid":false,"given":"Jingjie","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Research Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]},{"name":"GuangDong Engineering Technology Research Center of Fertility Preservation , Guangzhou,","place":["China"]},{"name":"Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,1,2]]},"reference":[{"key":"2026030500514792800_deaf250-B1","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1093\/humrep\/dew007","article-title":"Cryopreservation of in vitro matured oocytes in addition to ovarian tissue freezing for fertility preservation in paediatric female cancer patients before and after cancer therapy","volume":"31","author":"Abir","year":"2016","journal-title":"Hum Reprod"},{"key":"2026030500514792800_deaf250-B2","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1093\/biolre\/ioac024","article-title":"Oocyte mitochondria-key regulators of oocyte function and potential therapeutic targets for improving fertility","volume":"106","author":"Adhikari","year":"2022","journal-title":"Biol Reprod"},{"key":"2026030500514792800_deaf250-B3","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1007\/s10815-023-02945-2","article-title":"Preservation of fertility in female and male prepubertal patients diagnosed with cancer","volume":"40","author":"Albamonte","year":"2023","journal-title":"J Assist Reprod Genet"},{"key":"2026030500514792800_deaf250-B4","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq\u2014a Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"2026030500514792800_deaf250-B5","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1093\/humrep\/det388","article-title":"The immature human ovary shows loss of abnormal follicles and increasing follicle developmental competence through childhood and adolescence","volume":"29","author":"Anderson","year":"2014","journal-title":"Hum Reprod"},{"key":"2026030500514792800_deaf250-B6","doi-asserted-by":"crossref","first-page":"811","DOI":"10.3324\/haematol.2013.099747","article-title":"Hematopoietic stem cell transplantation in thalassemia major and sickle cell disease: indications and management recommendations from an international expert panel","volume":"99","author":"Angelucci","year":"2014","journal-title":"Haematologica"},{"key":"2026030500514792800_deaf250-B7","first-page":"271","article-title":"Cardiac magnetic resonance strain in beta thalassemia major correlates with cardiac iron overload","volume":"10","author":"Ansah","year":"2023","journal-title":"Children (Basel)"},{"key":"2026030500514792800_deaf250-B8","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1002\/ajh.21269","article-title":"Consensus statement on iron overload in myelodysplastic syndromes","volume":"83","author":"Bennett","year":"2008","journal-title":"Am J Hematol"},{"key":"2026030500514792800_deaf250-B9","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"2026030500514792800_deaf250-B10","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1093\/humrep\/deq146","article-title":"Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial","volume":"25","author":"Cobo","year":"2010","journal-title":"Hum Reprod"},{"key":"2026030500514792800_deaf250-B11","doi-asserted-by":"crossref","first-page":"451","DOI":"10.4103\/2230-8210.183456","article-title":"Endocrine profile of beta-thalassemia major patients followed from childhood to advanced adulthood in a tertiary care center","volume":"20","author":"De Sanctis","year":"2016","journal-title":"Indian J Endocrinol Metab"},{"key":"2026030500514792800_deaf250-B12","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1515\/jpem-2014-0242","article-title":"Anti-Mullerian hormone levels in American girls by age and race\/ethnicity","volume":"28","author":"Elchuri","year":"2015","journal-title":"J Pediatr Endocrinol Metab"},{"key":"2026030500514792800_deaf250-B13","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.tem.2012.04.003","article-title":"Iron metabolism and the polycystic ovary syndrome","volume":"23","author":"Escobar-Morreale","year":"2012","journal-title":"Trends Endocrinol Metab"},{"key":"2026030500514792800_deaf250-B14","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.rbmo.2017.03.007","article-title":"Outcomes of immature oocytes collected from ovarian tissue for cryopreservation in adult and prepubertal patients","volume":"34","author":"Fasano","year":"2017","journal-title":"Reprod Biomed Online"},{"key":"2026030500514792800_deaf250-B15","doi-asserted-by":"crossref","first-page":"hoae005","DOI":"10.1093\/hropen\/hoae005","article-title":"Neurotrophin-4 promotes in vitro development and maturation of human secondary follicles yielding metaphase II oocytes and successful blastocyst formation","volume":"2024","author":"Guo","year":"2024","journal-title":"Hum Reprod Open"},{"key":"2026030500514792800_deaf250-B16","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.1210\/jc.2010-0930","article-title":"Serum levels of anti-Mullerian hormone as a marker of ovarian function in 926 healthy females from birth to adulthood and in 172 Turner syndrome patients","volume":"95","author":"Hagen","year":"2010","journal-title":"J Clin Endocrinol Metab"},{"key":"2026030500514792800_deaf250-B17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.2010.05544.x","article-title":"Pathogenesis and management of iron toxicity in thalassemia","volume":"1202","author":"Hershko","year":"2010","journal-title":"Ann NY Acad Sci"},{"key":"2026030500514792800_deaf250-B18","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1210\/clinem\/dgab687","article-title":"Clinical utility of anti-Mullerian hormone in pediatrics","volume":"107","author":"Kanakatti Shankar","year":"2022","journal-title":"J Clin Endocrinol Metab"},{"key":"2026030500514792800_deaf250-B19","doi-asserted-by":"crossref","first-page":"D480","DOI":"10.1093\/nar\/gkm882","article-title":"KEGG for linking genomes to life and the environment","volume":"36","author":"Kanehisa","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"2026030500514792800_deaf250-B20","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.fertnstert.2019.03.026","article-title":"In vitro maturation rates in young premenarche patients","volume":"112","author":"Karavani","year":"2019","journal-title":"Fertil Steril"},{"key":"2026030500514792800_deaf250-B21","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat Methods"},{"key":"2026030500514792800_deaf250-B22","doi-asserted-by":"crossref","first-page":"2484","DOI":"10.3390\/cells10092484","article-title":"The role of mitochondria in oocyte maturation","volume":"10","author":"Kirillova","year":"2021","journal-title":"Cells"},{"key":"2026030500514792800_deaf250-B23","doi-asserted-by":"crossref","first-page":"115415","DOI":"10.1016\/j.biopha.2023.115415","article-title":"The emerging role of ferroptosis in female reproductive disorders","volume":"166","author":"Liu","year":"2023","journal-title":"Biomed Pharmacother"},{"key":"2026030500514792800_deaf250-B24","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"2026030500514792800_deaf250-B25","doi-asserted-by":"crossref","first-page":"593718","DOI":"10.3389\/fendo.2020.593718","article-title":"Consequences of beta-thalassemia or sickle cell disease for ovarian follicle number and morphology in girls who had ovarian tissue cryopreserved","volume":"11","author":"Mamsen","year":"2020","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2026030500514792800_deaf250-B26","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.3390\/antiox10121864","article-title":"Iron overload, oxidative stress, and ferroptosis in the failing heart and liver","volume":"10","author":"Mancardi","year":"2021","journal-title":"Antioxidants (Basel)"},{"key":"2026030500514792800_deaf250-B27","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.fertnstert.2017.05.024","article-title":"Update on fertility preservation from the Barcelona International Society for Fertility Preservation-ESHRE-ASRM 2015 expert meeting: indications, results and future perspectives","volume":"108","author":"Martinez","year":"2017","journal-title":"Fertil Steril"},{"key":"2026030500514792800_deaf250-B28","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/ng1180","article-title":"PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes","volume":"34","author":"Mootha","year":"2003","journal-title":"Nat Genet"},{"key":"2026030500514792800_deaf250-B29","doi-asserted-by":"crossref","first-page":"159","DOI":"10.4274\/jtgga.galenos.2023.2022-12-2","article-title":"Assessment of the ovarian reserve in patients with beta-thalassemia major: a prospective longitudinal study","volume":"24","author":"Ozcan","year":"2023","journal-title":"J Turkish German Gynecol Assoc"},{"key":"2026030500514792800_deaf250-B30","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1111\/bjh.13497","article-title":"Gonadal function and fertility after stem cell transplantation in childhood: comparison of a reduced intensity conditioning regimen containing melphalan with a myeloablative regimen containing busulfan","volume":"170","author":"Panasiuk","year":"2015","journal-title":"Br J Haematol"},{"key":"2026030500514792800_deaf250-B31","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1006\/cryo.1999.2170","article-title":"Cryopreservation of bovine ovarian tissue: structural normality of follicles after thawing and culture in vitro","volume":"38","author":"Paynter","year":"1999","journal-title":"Cryobiology"},{"key":"2026030500514792800_deaf250-B32","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1016\/j.fertnstert.2019.09.013","article-title":"Fertility preservation in patients undergoing gonadotoxic therapy or gonadectomy: a committee opinion","volume":"112","author":"Practice Committee of the American Society for Reproductive Medicine. Electronic address","year":"2019","journal-title":"Fertil Steril"},{"key":"2026030500514792800_deaf250-B33","doi-asserted-by":"crossref","first-page":"R22","DOI":"10.1186\/gb-2011-12-3-r22","article-title":"Improving RNA-seq expression estimates by correcting for fragment bias","volume":"12","author":"Roberts","year":"2011","journal-title":"Genome Biol"},{"key":"2026030500514792800_deaf250-B34","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1186\/s13048-023-01300-4","article-title":"The stromal microenvironment and ovarian aging: mechanisms and therapeutic opportunities","volume":"16","author":"Shen","year":"2023","journal-title":"J Ovarian Res"},{"key":"2026030500514792800_deaf250-B35","doi-asserted-by":"crossref","first-page":"2878","DOI":"10.1182\/blood-2011-06-360271","article-title":"Reproductive capacity in iron overloaded women with thalassemia major","volume":"118","author":"Singer","year":"2011","journal-title":"Blood"},{"key":"2026030500514792800_deaf250-B36","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026030500514792800_deaf250-B37","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.ejogrb.2019.04.046","article-title":"A 10-year longitudinal study of evaluation of ovarian reserve in women with transfusion-dependent beta thalassaemia major","volume":"238","author":"Talaulikar","year":"2019","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"2026030500514792800_deaf250-B38","doi-asserted-by":"crossref","first-page":"D330","DOI":"10.1093\/nar\/gky1055","article-title":"The Gene Ontology Resource: 20 years and still GOing strong","volume":"47","author":"The Gene Ontology Consortium","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2026030500514792800_deaf250-B39","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1053\/ybeog.2003.0360","article-title":"Disorders of pubertal development","volume":"17","author":"Traggiai","year":"2003","journal-title":"Best Pract Res Clin Obstet Gynaecol"},{"key":"2026030500514792800_deaf250-B40","doi-asserted-by":"crossref","first-page":"101074","DOI":"10.1016\/j.blre.2023.101074","article-title":"Thalassaemia in China","volume":"60","author":"Wang","year":"2023","journal-title":"Blood Rev"},{"key":"2026030500514792800_deaf250-B41","first-page":"100141","article-title":"clusterProfiler 4.0: a universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation (Camb)"},{"key":"2026030500514792800_deaf250-B42","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1080\/14647273.2017.1422297","article-title":"Fertility preservation for medical reasons in girls and women: British fertility society policy and practice guideline","volume":"21","author":"Yasmin","year":"2018","journal-title":"Hum Fertil (Camb)"}],"container-title":["Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/humrep\/article-pdf\/41\/3\/410\/66210903\/deaf250.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/humrep\/article-pdf\/41\/3\/410\/66210903\/deaf250.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T05:52:04Z","timestamp":1772689924000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/humrep\/article\/41\/3\/410\/8408566"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,2]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,1,2]]},"published-print":{"date-parts":[[2026,3,1]]}},"URL":"https:\/\/doi.org\/10.1093\/humrep\/deaf250","relation":{},"ISSN":["0268-1161","1460-2350"],"issn-type":[{"value":"0268-1161","type":"print"},{"value":"1460-2350","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,3]]},"published":{"date-parts":[[2026,1,2]]}}}