{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:20:09Z","timestamp":1790684409713,"version":"4.1.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,1]]},"DOI":"10.1093\/humrep\/del506","type":"journal-article","created":{"date-parts":[[2007,2,2]],"date-time":"2007-02-02T12:15:39Z","timestamp":1170418539000},"page":"1443-1449","source":"Crossref","is-referenced-by-count":140,"title":["Blastocyst biopsy versus cleavage stage biopsy and blastocyst transfer for preimplantation genetic diagnosis of \u03b2-thalassaemia: a pilot study"],"prefix":"10.1093","volume":"22","author":[{"given":"G.","family":"Kokkali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Traeger-Synodinos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Vrettou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Stavrou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.M.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.S.","family":"Cram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Makrakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.O.","family":"Trounson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Kanavakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Pantos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,1,29]]},"reference":[{"key":"key\n\t\t\t\t20171012131641_del506c1","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1002\/pd.111","article-title":"Preimplantation genetic diagnosis of the fragile X syndrome by use of polymorphic linked markers","volume":"21","author":"Apessos","year":"2001","journal-title":"Prenat Diagn"},{"key":"key\n\t\t\t\t20171012131641_del506c2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.fertnstert.2004.06.049","article-title":"Blastocyst culture after repeated failure of cleavage-stage embryo transfers: a comparison of day 5 and day 6 transfers","volume":"83","author":"Barrenetxea","year":"2005","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c3","first-page":"109","article-title":"Approaches to sex selection in farm animals","volume-title":"New Technologies in Animal Breeding","author":"Betteridge","year":"1981"},{"key":"key\n\t\t\t\t20171012131641_del506c4","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1093\/humrep\/deh104","article-title":"The merits of blastocyst versus cleavage stage embryo transfer: a Cochrane review","volume":"19","author":"Blake","year":"2004","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c5","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.fertnstert.2003.11.064","article-title":"Moving to blastocyst biopsy for PGD and single embryo transfer at Sydney IVF","volume":"82","author":"de Boer","year":"2004","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c6","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1023\/A:1022548612107","article-title":"Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: report of two cases","volume":"15","author":"Boada","year":"1998","journal-title":"J Assist Reprod Genet"},{"key":"key\n\t\t\t\t20171012131641_del506c7","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1093\/humrep\/12.2.328","article-title":"Use of synthetic serum substitute and \u03b1-minimum essential medium for the extended culture of human embryos to the blastocyst stage","volume":"12","author":"Desai","year":"1997","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c8","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1093\/oxfordjournals.humrep.a137191","article-title":"Trophoectoderm biopsy in human blastocysts","volume":"5","author":"Dorkas","year":"1990","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c9","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/molehr\/6.5.391","article-title":"Multiplex PCR of polymorphic markers flanking the CFTR gene: a general approach for preimplantation genetic diagnosis of cystic fibrosis","volume":"6","author":"Dreesen","year":"2000","journal-title":"Mol Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c10","first-page":"279","article-title":"Manipulations on the blastocyst","volume":"6","author":"Gardner","year":"1971","journal-title":"Adv Biosci"},{"key":"key\n\t\t\t\t20171012131641_del506c11","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1038\/218346a0","article-title":"Control of the sex ratio at full term in the rabbit by transferring sexed blastocysts","volume":"218","author":"Gardner","year":"1968","journal-title":"Nature"},{"key":"key\n\t\t\t\t20171012131641_del506c12","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1093\/humrep\/13.suppl_3.148","article-title":"Culture of viable human blastocysts in defined sequential serum-free media media","volume":"13","author":"Gardner","year":"1998","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c13","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/S0015-0282(97)00438-X","article-title":"Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers","volume":"69","author":"Gardner","year":"1998","journal-title":"Fert Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c14","doi-asserted-by":"crossref","first-page":"3434","DOI":"10.1093\/humrep\/13.12.3434","article-title":"A prospective randomised trail of blastocyst culture and transfer in in vitro fertilisation","volume":"13","author":"Gardner","year":"1998","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c15","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1002\/1097-0223(200007)20:7<571::AID-PD879>3.0.CO;2-N","article-title":"Cinical application of preimplantation genetic diagnosis for cystic fibrosis","volume":"20","author":"Goossens","year":"2000","journal-title":"Prenat Diagn"},{"key":"key\n\t\t\t\t20171012131641_del506c16","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1093\/humrep\/13.6.1656","article-title":"Successful outcome with day 4 embryo transfer after preimplantation diagnosis for genetically transmitted diseases","volume":"13","author":"Grifo","year":"1998","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c17","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1093\/humrep\/13.11.3151","article-title":"Mosaicism in the inner cell mass of human blastocysts","volume":"13","author":"Evsikov","year":"1998","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c18","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/344768a0","article-title":"Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification","volume":"344","author":"Handyside","year":"1990","journal-title":"Nature"},{"key":"key\n\t\t\t\t20171012131641_del506c19","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/humrep\/dei292","article-title":"ESHRE PGD Consortium data collection V: cycles from January to December 2002 with pregnancy follow-up to October 2003","volume":"21","author":"Harper","year":"2006","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c20","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/humrep\/13.1.169","article-title":"Evolution of a culture protocol for successful blastocyst development and pregnancy","volume":"13","author":"Jones","year":"1998","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c21","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1016\/S0015-0282(98)00342-2","article-title":"Factors affecting the success of human blastocyst development and pregnancy following in vitro fertilisation and embryo transfer","volume":"79","author":"Jones","year":"1998","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c22","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1093\/humrep\/14.6.1405","article-title":"Blastocyst stage transfer: pitfalls and benefits. The benefits of extended culture","volume":"14","author":"Jones","year":"1999","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c23","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1136\/jmg.39.1.6","article-title":"Preimplanation genetic diagnosis in clinical practice","volume":"39","author":"Kanavakis","year":"2002","journal-title":"J Med Genet"},{"key":"key\n\t\t\t\t20171012131641_del506c24","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1093\/humrep\/deh893","article-title":"Birth of a healthy infant following trophectoderm biopsy from blastocysts for preimplantation diagnosis of \u03b2-thalassaemia major","volume":"20","author":"Kokkali","year":"2005","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c25","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1023\/A:1022571822585","article-title":"Preimplantation diagnosis of thalassemias","volume":"15","author":"Kuliev","year":"1998","journal-title":"J Assist Reprod Genet"},{"key":"key\n\t\t\t\t20171012131641_del506c26","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1016\/j.fertnstert.2005.05.063","article-title":"Pregnancies and live births after trophectoderm biopsy and preimplantation genetic testing of human blastocysts","volume":"84","author":"McArthur","year":"2005","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c27","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1093\/humrep\/15.8.1781","article-title":"Chromosome mosaicism in day 3 aneuploid embryos that develop to morphologically normal blastocyst in vitro","volume":"15","author":"Magli","year":"2000","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c28","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1093\/oxfordjournals.humrep.a136711","article-title":"Preimplantation diagnosis of HPRT-deficient male and carrier female mouse embryos by trophectoderm biopsy","volume":"3","author":"Monk","year":"1988","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c29","doi-asserted-by":"crossref","first-page":"2976","DOI":"10.1093\/oxfordjournals.humrep.a135833","article-title":"A retrospective analysis of the in-vitro development of \u201cspare\u201d human in-vitro fertilization preimplantation embryos using \u201cin-house\u201d prepared medium and \u201cMedi-cult\u201d commercial medium","volume":"10","author":"Muggleton-Harris","year":"1995","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c30","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1093\/humrep\/17.1.25","article-title":"Pregnancies following blastocyst stage transfer in PGD cycles at risk for \u03b2-thalassaemic haemoglobinopathies","volume":"17","author":"Palmer","year":"2002","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c31","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0015-0282(16)56650-3","article-title":"Prospective study of a modified gonadotropin-releasing hormone agonist long protocol in an in vitro fertilization program","volume":"61","author":"Pantos","year":"1994","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c32","first-page":"113","article-title":"The successful use of hatched blastocysts in assisted reproductive technology","volume":"28","author":"Pantos","year":"2001","journal-title":"Clin Exp Obstet Gynecol"},{"key":"key\n\t\t\t\t20171012131641_del506c33","first-page":"42","article-title":"Blastocyst versus early cleavage embryo transfer: a retrospective analysis of 4165 transfers","volume":"31","author":"Pantos","year":"2004","journal-title":"Clin Exp Obstet Gynecol"},{"key":"key\n\t\t\t\t20171012131641_del506c34","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.fertnstert.2003.07.016","article-title":"Comparison of embryo transfer on day 2, day 3 and day 6: A prospective randomized study","volume":"81","author":"Pantos","year":"2004","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c35","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1056\/NEJMoa053524","article-title":"In vitro fertilization with single blastocyst-stage versus single cleavage-stage embryos","volume":"354","author":"Papanikolaou","year":"2006","journal-title":"N Engl J Med"},{"key":"key\n\t\t\t\t20171012131641_del506c36","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1002\/pd.170","article-title":"PGD protocols for alpha and beta-thalassaemias using multiplex fluorescent PCR","volume":"21","author":"Piyamongkol","year":"2001","journal-title":"Prenat Diagn"},{"key":"key\n\t\t\t\t20171012131641_del506c37","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1002\/(SICI)1097-0223(199812)18:13<1402::AID-PD500>3.0.CO;2-T","article-title":"Assessment of the reliability of single blastomere analysis for preimplanation genetic diagnosis of the \u0394F508 deletion causing cystic fibrosis in clinical practice","volume":"18","author":"Ray","year":"1998","journal-title":"Prenat Diagn"},{"key":"key\n\t\t\t\t20171012131641_del506c38","doi-asserted-by":"crossref","first-page":"2869","DOI":"10.1093\/humrep\/13.10.2869","article-title":"The predictive value of day 3 embryo morphology regarding blastocyst formation, pregnancy and implantation rate after day 5 transfer following in-vitro fertilisation or intracytoplasmic sperm injection","volume":"13","author":"Rijnders","year":"1998","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c39","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1016\/S0015-0282(16)58349-6","article-title":"A prospective, randomised study of embryo transfer results after 3 or 5 days of embryo culture in in vitro fertilisation","volume":"65","author":"Scholtes","year":"1996","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c40","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/S0015-0282(99)00311-8","article-title":"Blastocyst culture and transfer: analysis of results and parameters affecting outcome in two in vitro fertilisation programs","volume":"72","author":"Schoolcraft","year":"1999","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c41","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1016\/S0140-6736(04)16209-0","article-title":"Preimplantation genetic diagnosis","volume":"363","author":"Sermon","year":"2004","journal-title":"Lancet"},{"key":"key\n\t\t\t\t20171012131641_del506c42","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1016\/S0015-0282(01)01771-X","article-title":"A comparison of day 5 and day 6 blastocyst transfers","volume":"75","author":"Shapiro","year":"2001","journal-title":"Fertil Steril"},{"key":"key\n\t\t\t\t20171012131641_del506c43","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1093\/humrep\/deh536","article-title":"Comparison of blastocyst transfer with or without preimplantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: a prospective randomised controlled trial","volume":"19","author":"Staessen","year":"2004","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c44","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1093\/oxfordjournals.humrep.a136713","article-title":"Normal in vivo development of marmoset monkey embryos after trophoectoderm biopsy","volume":"3","author":"Summers","year":"1988","journal-title":"Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c45","article-title":"Blastocyst biopsy improved implantation in infertile patient with advanced maternal age","author":"Tongyai","year":"2004"},{"key":"key\n\t\t\t\t20171012131641_del506c46","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1093\/molehr\/gag038","article-title":"An evaluation of preimplantation genetic diagnosis in clinical genetic services through three years application for prevention of \u03b2-thalassaemia major and sickle cell thalassaemia","volume":"9","author":"Traeger-Synodinos","year":"2003","journal-title":"Mol Hum Reprod"},{"key":"key\n\t\t\t\t20171012131641_del506c47","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/BF02072538","article-title":"Polar body diagnosis of common aneuploidies by FISH","volume":"13","author":"Verlinsky","year":"1996","journal-title":"J Assist Reprod Genet"},{"key":"key\n\t\t\t\t20171012131641_del506c48","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/bmme.1997.2635","article-title":"Preimplantation diagnosis of single gene disorders by two-step oocyte genetic analysis using first and second polar body","volume":"62","author":"Verlinsky","year":"1997","journal-title":"Biochem Mol Med"},{"key":"key\n\t\t\t\t20171012131641_del506c49","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1017\/S0967199400003920","article-title":"Laser blastocyst biopsy for preimplantation diagnosis in the human","volume":"5","author":"Veiga","year":"1997","journal-title":"Zygote"},{"key":"key\n\t\t\t\t20171012131641_del506c50","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1373\/49.5.769","article-title":"Rapid screening of multiple \u03b2-globin gene mutations by real-time PCR on the Lightcycler: application to carrier screening and prenatal diagnosis of thalassemia syndromes","volume":"49","author":"Vrettou","year":"2003","journal-title":"Clin Chem"},{"key":"key\n\t\t\t\t20171012131641_del506c51","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1002\/humu.20022","article-title":"Real-time PCR for single-cell genotyping in sickle cell and thalassaemia syndromes as a rapid, accurate, reliable and widely applicable protocol for PGD","volume":"23","author":"Vrettou","year":"2004","journal-title":"Hum Mutat"},{"key":"key\n\t\t\t\t20171012131641_del506c52","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S1472-6483(10)60947-9","article-title":"Intracytoplasmic injection of spermatozoa and spermatogenic cells: its biology and applications in humans and animals","volume":"10","author":"Yanagimachi","year":"2005","journal-title":"Reprod Biomed Online"}],"container-title":["Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/humrep\/article-pdf\/22\/5\/1443\/9543876\/del506.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T13:29:59Z","timestamp":1507814999000},"score":1,"resource":{"primary":{"URL":"http:\/\/academic.oup.com\/humrep\/article\/22\/5\/1443\/2913866\/Blastocyst-biopsy-versus-cleavage-stage-biopsy-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,29]]},"references-count":52,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2007,1,29]]},"published-print":{"date-parts":[[2007,5,1]]}},"URL":"https:\/\/doi.org\/10.1093\/humrep\/del506","relation":{},"ISSN":["1460-2350","0268-1161"],"issn-type":[{"value":"1460-2350","type":"electronic"},{"value":"0268-1161","type":"print"}],"subject":[],"published-other":{"date-parts":[[2007,5]]},"published":{"date-parts":[[2007,1,29]]}}}