{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:21:05Z","timestamp":1792794065220,"version":"4.5.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2019,10,8]],"date-time":"2019-10-08T00:00:00Z","timestamp":1570492800000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"European Union, Interreg V \u00d6KS","id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>STUDY QUESTION<\/jats:title>\n                    <jats:p>Can ovarian biopsying per se and\/or autotransplantation of fragmented ovarian cortical tissue activate dormant follicles and increase the number of recruitable follicles for IVF\/ICSI in women with diminished ovarian reserve (DOR)?<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>SUMMARY ANSWER<\/jats:title>\n                    <jats:p>Ovarian biopsying followed by immediate autotransplantation of fragmented cortical tissue failed to increase the number of recruitable follicles for IVF\/ICSI 10\u00a0weeks after the procedure either at the graft site or in the biopsied ovary, but 12 of the 20 women subsequently had a clinical pregnancy during the 1-year follow-up.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>WHAT IS KNOWN ALREADY<\/jats:title>\n                    <jats:p>Infertile women with DOR constitute a group of patients with poor reproductive outcome mainly due to the low number of mature oocytes available for IVF\/ICSI. Recent studies have shown that in vitro activation of residual dormant follicles by both chemical treatment and tissue fragmentation has resulted in return of menstrual cycles and pregnancies in a fraction of amenorrhoeic women with premature ovarian insufficiency.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN, SIZE, DURATION<\/jats:title>\n                    <jats:p>This is a prospective clinical cohort study including 20 women with DOR treated at the fertility clinic, Rigshospitalet, Denmark, during April 2016\u2013December 2017. Non-pregnant patients were on average followed for 280\u00a0days (range 118\u2013408), while women who conceived were followed until delivery. Study follow-up of non-pregnant patients ended in September 2018.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>PARTICIPANTS, MATERIALS, SETTING, METHODS<\/jats:title>\n                    <jats:p>The study included infertile women aged 30\u201339\u00a0years with preserved menstrual cycles, indication for IVF\/ICSI and repeated serum measurements of anti-M\u00fcllerian hormone (AMH)\u2009\u2264\u20095\u00a0pmol\/L. Patients were randomized to have four biopsies taken from either the left or the right ovary by laparoscopy followed by fragmentation of the cortical tissue to an approximate size of 1\u00a0mm3 and autotransplanted to a peritoneal pocket. The other ovary served as a control. Patients were followed weekly for 10\u00a0weeks with recording of hormone profile, antral follicle count (AFC), ovarian volume and assessment for ectopic follicle growth. After 10\u00a0weeks, an IVF\/ICSI-cycle with maximal ovarian stimulation was initiated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>MAIN RESULTS AND THE ROLE OF CHANCE<\/jats:title>\n                    <jats:p>No difference in the number of mature follicles after ovarian stimulation 10\u00a0weeks after the procedure in the biopsied versus the control ovaries was observed (1.0 vs. 0.7 follicles, P\u00a0=\u20090.35). In only three patients, growth of four follicles was detected at the graft site 24\u2013268\u00a0days after the procedure. From one of these follicles, a metaphase II (MII) oocyte was retrieved and fertilized, but embryonic development failed. Overall AMH levels did not change significantly after the procedure (P\u00a0=\u20090.2). The AFC increased by 0.14 (95% CI: 0.06;0.21) per week (P\u00a0&lt;\u20090.005), and the biopsied ovary had on average 0.6 (95% CI: 0.3;\u22120.88) follicles fewer than the control ovary (P\u00a0=\u20090.01). Serum levels of androstenedione and testosterone increased significantly by 0.63\u00a0nmol\/L (95% CI: 0.21;1.04) and 0.11\u00a0nmol\/L (95% CI: 0.01;0.21) 1\u00a0week after the procedure, respectively, and testosterone increased consecutively over the 10\u00a0weeks by 0.0095\u00a0nmol\/L (95% CI: 0.0002;0.0188) per week (P\u00a0=\u20090.045). In 7 of the 20 patients, there was a serum AMH elevation 5 to 8\u00a0weeks after the procedure. In this group, mean AMH increased from 2.08\u00a0pmol\/L (range 1.74\u20132.34) to 3.94\u00a0pmol\/L (range 3.66\u20134.29) from Weeks 1\u20134 to Weeks 5\u20138. A clinical pregnancy was obtained in 12 of the 20 (60%) patients with and without medically assisted reproduction (MAR) treatments. We report a cumulated live birth rate per started IVF\/ICSI cycle of 18.4%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>LIMITATIONS, REASON FOR CAUTION<\/jats:title>\n                    <jats:p>Limitations of the study were the number of patients included and the lack of a non-operated control group. Moreover, 9 of the 20 women had no male partner at inclusion and were treated with donor sperm, but each of these women had an average of 6.8 (range 4\u20139) unsuccessful MAR treatments with donor sperm prior to inclusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>WIDER IMPLICATIONS OF THE FINDINGS<\/jats:title>\n                    <jats:p>Although 12 out of 20 patients became pregnant during the follow-up period, the current study does not indicate that biopsying, fragmenting and autotransplanting of ovarian cortical tissue increase the number of recruitable follicles for IVF\/ICSI after 10\u00a0weeks. However, a proportion of the patients may have a follicular response in Weeks 5\u20138 after the procedure. It could therefore be relevant to perform a future study on the possible effects of biopsying per se that includes stimulation for IVF\/ICSI earlier than week 10.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY FUNDING\/COMPETING INTEREST(S)<\/jats:title>\n                    <jats:p>This study is part of the ReproUnion collaborative study, co-financed by the European Union, Interreg V \u00d6KS. The funders had no role in the study design, data collection and interpretation, or decision to submit the work for publication. None of the authors have a conflict of interest.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>TRIAL REGISTRATION NUMBER<\/jats:title>\n                    <jats:p>NCT02792569.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/humrep\/dez152","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T15:11:18Z","timestamp":1562771478000},"page":"1924-1936","source":"Crossref","is-referenced-by-count":54,"title":["Biopsying, fragmentation and autotransplantation of fresh ovarian cortical tissue in infertile women with diminished ovarian reserve"],"prefix":"10.1093","volume":"34","author":[{"given":"Stine Aagaard","family":"Lunding","sequence":"first","affiliation":[{"name":"The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne Elisabeth","family":"Pors","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stine Gry","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma Kloeve","family":"Landersoe","sequence":"additional","affiliation":[{"name":"The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janni Vikkels\u00f8","family":"Jeppesen","sequence":"additional","affiliation":[{"name":"The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esben Meulengracht","family":"Flachs","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Medicine, Copenhagen University Hospital, Bispebjerg Hospital, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Pinborg","sequence":"additional","affiliation":[{"name":"The Fertility Clinic, Department of Obstetrics and Gynaecology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten Tryde","family":"Macklon","sequence":"additional","affiliation":[{"name":"The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette T\u00f8nnes","family":"Pedersen","sequence":"additional","affiliation":[{"name":"The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"},{"name":"Department of Gynaecology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus Yding","family":"Andersen","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders Nyboe","family":"Andersen","sequence":"additional","affiliation":[{"name":"The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,10,8]]},"reference":[{"key":"2019102315500240100_ref1","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s11845-017-1665-1","article-title":"Association of basal serum androgen levels with ovarian response and ICSI cycle outcome","volume":"187","author":"Abide Yayla","year":"2018","journal-title":"Ir J Med Sci"},{"key":"2019102315500240100_ref2","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1210\/jc.2012-1829","article-title":"Ovarian antral follicle subclasses and anti-M\u00fcllerian hormone during normal reproductive aging","volume":"98","author":"Bentzen","year":"2013","journal-title":"J Clin Endocrinol Metab"},{"key":"2019102315500240100_ref3","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.rbmo.2017.03.009","article-title":"Live birth rates in various subgroups of poor ovarian responders fulfilling the Bologna criteria","volume":"34","author":"Bozdag","year":"2017","journal-title":"Reprod Biomed Online [Internet]"},{"key":"2019102315500240100_ref4","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1093\/humrep\/deu319","article-title":"A retrospective evaluation of prognosis and cost-effectiveness of IVF in poor responders according to the Bologna criteria","volume":"30","author":"Busnelli","year":"2015","journal-title":"Hum Reprod"},{"key":"2019102315500240100_ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12958-018-0409-z","article-title":"Dehydroepiandrosterone (DHEA) supplementation improves in vitro fertilization outcomes of poor ovarian responders, especially in women with low serum concentration of DHEA-S: a retrospective cohort study","volume":"16","author":"Chern","year":"2018","journal-title":"Reprod Biol Endocrinol"},{"key":"2019102315500240100_ref6","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1148\/radiology.177.1.2204964","article-title":"Ovarian volumes measured by US: bigger than we think","author":"Cohen","year":"1990","journal-title":"Radiology"},{"key":"2019102315500240100_ref7","first-page":"6649","article-title":"Orthotopic and heterotopic ovarian tissue transplantation","volume":"15","author":"Demeestere","year":"2009","journal-title":"Hum Reprod Updat [Internet]"},{"key":"2019102315500240100_ref8","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/j.fertnstert.2013.03.030","article-title":"Restoration of ovarian activity and pregnancy after transplantation of cryopreserved ovarian tissue: a review of 60 cases of reimplantation","volume":"99","author":"Donnez","year":"2013","journal-title":"Fertil Steril [Internet]"},{"key":"2019102315500240100_ref9","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/S0140-6736(04)17222-X","article-title":"Livebirth after orthotopic transplantation of cryopreserved ovarian tissue","volume":"364","author":"Donnez","year":"2004","journal-title":"Lancet (London, England) [Internet]"},{"key":"2019102315500240100_ref10","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1038\/nature10137","article-title":"Role of YAP\/TAZ in mechanotransduction","volume":"474","author":"Dupont","year":"2011","journal-title":"Nature [Internet]"},{"key":"2019102315500240100_ref11","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1210\/er.2009-0012","article-title":"The mammalian ovary from genesis to revelation","volume":"30","author":"Edson","year":"2009","journal-title":"Endocr Rev"},{"key":"2019102315500240100_ref12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13048-018-0447-3","article-title":"Pregnancy after drug-free in vitro activation of follicles and fresh tissue autotransplantation in primary ovarian insufficiency patient: a case report and literature review","volume":"11","author":"Fabregues","year":"2018","journal-title":"J Ovarian Res"},{"key":"2019102315500240100_ref13","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1093\/humrep\/der092","article-title":"ESHRE consensus on the definition of \u201cpoor response\u201d to ovarian stimulation for in vitro fertilization: the Bologna criteria","volume":"26","author":"Ferraretti","year":"2011","journal-title":"Hum Reprod [Internet]"},{"key":"2019102315500240100_ref14","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3109\/09513590.2014.964639","article-title":"Ovarian and adrenal androgens may be useful markers to predict oocyte competence and embryo development in older women","volume":"31","author":"Ferrario","year":"2015","journal-title":"Gynecol Endocrinol"},{"key":"2019102315500240100_ref15","first-page":"620","article-title":"The impact of genetic variation and gene expression level of the follicle-stimulating hormone receptor on ovarian reserve","author":"Ghezelayagh","year":"2016"},{"key":"2019102315500240100_ref16","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/rda.13357","article-title":"Vitrification of cat ovarian tissue: does fragment size matters?","volume":"53","author":"Gorricho","year":"2018","journal-title":"Reprod Domest Anim [Internet]"},{"key":"2019102315500240100_ref17","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.ando.2010.02.021","article-title":"Human ovarian follicular development: from activation of resting follicles to preovulatory maturation","volume":"71","author":"Gougeon","year":"2010","journal-title":"Ann Endocrinol (Paris) [Internet]"},{"key":"2019102315500240100_ref18","first-page":"1","article-title":"Autologous stem cell ovarian transplantation to increase reproductive potential in patients who are poor responders","author":"Herraiz","year":"2018","journal-title":"Fertil Steril"},{"key":"2019102315500240100_ref19","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1093\/humrep\/dex208","article-title":"Efficacy and safety of follitropin alfa\/lutropin alfa in ART: a randomized controlled trial in poor ovarian responders","volume":"32","author":"Humaidan","year":"2017","journal-title":"Hum Reprod"},{"key":"2019102315500240100_ref20","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1093\/molehr\/gat024","article-title":"Which follicles make the most anti-M?? Llerian hormone in humans? Evidence for an abrupt decline in AMH production at the time of follicle selection","volume":"19","author":"Jeppesen","year":"2013","journal-title":"Mol Hum Reprod [Internet]"},{"key":"2019102315500240100_ref21","doi-asserted-by":"crossref","first-page":"70","DOI":"10.4103\/0974-1208.183515","article-title":"Effective treatment protocol for poor ovarian response: a systematic review and meta-analysis","volume":"9","author":"Jeve","year":"2016","journal-title":"J Hum Reprod Sci [Internet]"},{"key":"2019102315500240100_ref22","doi-asserted-by":"crossref","first-page":"17474","DOI":"10.1073\/pnas.1312830110","article-title":"Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment","volume":"110","author":"Kawamura","year":"2013","journal-title":"Proc Natl Acad Sci U S A [Internet]"},{"key":"2019102315500240100_ref23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fendo.2017.00376","article-title":"Hallmarks of human small antral follicle development: implications for regulation of ovarian steroidogenesis and selection of the dominant follicle","volume":"8","author":"Kristensen","year":"2018","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2019102315500240100_ref24","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1091\/mbc.e10-01-0066","article-title":"Cortical mechanics and meiosis II completion in mammalian oocytes are mediated by myosin-II and Ezrin-Radixin-Moesin (ERM) proteins","volume":"21","author":"Larson","year":"2010","journal-title":"Mol Biol Cell"},{"key":"2019102315500240100_ref25","doi-asserted-by":"crossref","first-page":"2796","DOI":"10.1093\/humrep\/dew248","article-title":"Comparative evaluation of three new commercial immunoassays for anti-M\u00fcllerian hormone measurement","volume":"31","author":"Li","year":"2016","journal-title":"Hum Reprod [Internet]"},{"key":"2019102315500240100_ref26","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/j.fertnstert.2018.08.024","article-title":"Autotransplantation of fragmented ovarian cortical tissue: a laparoscopic demonstration","volume":"110","author":"Lunding","year":"2018","journal-title":"Fertil Steril [Internet]"},{"key":"2019102315500240100_ref27","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1007\/s10815-015-0476-4","article-title":"Live birth rates in the different combinations of the Bologna criteria poor ovarian responders: a validation study","volume":"32","author":"La Marca","year":"2015","journal-title":"J Assist Reprod Genet"},{"key":"2019102315500240100_ref28","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1210\/jc.2003-030217","article-title":"Delayed puberty and primary amenorrhea associated with a novel mutation of the human follicle-stimulating hormone receptor: clinical, histological, and molecular studies","volume":"88","author":"Meduri","year":"2003","journal-title":"J Clin Endocrinol Metab"},{"key":"2019102315500240100_ref29","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1056\/NEJMc055237","article-title":"Pregnancy after transplantation of cryopreserved ovarian","volume":"353","author":"Meirow","year":"2005","journal-title":"N Engl J Med [Internet]"},{"key":"2019102315500240100_ref30","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1093\/molehr\/gaq073","article-title":"In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA","volume":"17","author":"Nielsen","year":"2011","journal-title":"Mol Hum Reprod"},{"key":"2019102315500240100_ref31","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1093\/humupd\/dmw001","article-title":"Trends in \u201cpoor responder\u201d research: lessons learned from RCTs in assisted conception","volume":"22","author":"Papathanasiou","year":"2016","journal-title":"Hum Reprod Update"},{"key":"2019102315500240100_ref32","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s10815-017-1058-4","article-title":"Reproductive ovarian testing and the alphabet soup of diagnoses: DOR, POI, POF, POR, and FOR","volume":"35","author":"Pastore","year":"2018","journal-title":"J Assist Reprod Genet"},{"key":"2019102315500240100_ref33","author":"Pfleger","year":"2017"},{"key":"2019102315500240100_ref34","article-title":"Actin cytoskeleton regulates hippo signaling","volume":"8","author":"Reddy","year":"2013","journal-title":"PLoS One [Internet]"},{"key":"2019102315500240100_ref35","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1093\/humrep\/deg246","article-title":"Density and distribution of primordial follicles in single pieces of cortex from 21 patients and in individual pieces of cortex from three entire human ovaries","volume":"18","author":"Schmidt","year":"2003","journal-title":"Hum Reprod [Internet]"},{"key":"2019102315500240100_ref36","doi-asserted-by":"crossref","first-page":"3539","DOI":"10.1093\/humrep\/dei250","article-title":"Follow-up of ovarian function post-chemotherapy following ovarian cryopreservation and transplantation","volume":"20","author":"Schmidt","year":"2005","journal-title":"Hum Reprod"},{"key":"2019102315500240100_ref37","doi-asserted-by":"crossref","first-page":"3008","DOI":"10.1073\/pnas.1318978111","article-title":"Androgens regulate ovarian follicular development by increasing follicle stimulating hormone receptor and microRNA-125b expression","volume":"111","author":"Sen","year":"2014","journal-title":"Proc Natl Acad Sci [Internet]"},{"key":"2019102315500240100_ref38","first-page":"1","article-title":"Biomechanics and mechanical signaling in the ovary: a systematic review","author":"Shah","year":"2018","journal-title":"J Assist Reprod Genet"},{"key":"2019102315500240100_ref39","first-page":"30","article-title":"Melnick J","author":"Silber","year":"2018"},{"key":"2019102315500240100_ref40","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1073\/pnas.0810012106","article-title":"Inferring biological mechanisms from spatial analysis: prediction of a local inhibitor in the ovary","volume":"106","author":"Da Silva-Buttkus","year":"2009","journal-title":"Proc Natl Acad Sci U S A [Internet]"},{"key":"2019102315500240100_ref41","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0019475","article-title":"Enhancement of neoangiogenesis and follicle survival by sphingosine-1-phosphate in human ovarian tissue xenotransplants","volume":"6","author":"Soleimani","year":"2011","journal-title":"PLoS One"},{"key":"2019102315500240100_ref42","first-page":"608","article-title":"Successful fertility preservation following ovarian tissue vitrification in patients with primary ovarian insufficiency","volume":"30","author":"Suzuki","year":"2015","journal-title":"Hum Reprod Adv Access Publ January [Internet]"},{"key":"2019102315500240100_ref43","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1017\/S0967199411000268","article-title":"Effect of androstenedione on the growth and meiotic competence of bovine oocytes from early antral follicles","volume":"20","author":"Taketsuru","year":"2012","journal-title":"Zygote"},{"key":"2019102315500240100_ref44","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1093\/molehr\/gap073","article-title":"The primordial pool of follicles and nest breakdown in mammalian ovaries","volume":"15","author":"Tingen","year":"2009","journal-title":"Mol Hum Reprod"},{"key":"2019102315500240100_ref45","article-title":"Bologna criteria are predictive for ovarian response and live birth in subsequent ovarian stimulation cycles","volume-title":"Urman B","author":"Yakin","year":"2018"},{"key":"2019102315500240100_ref46","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1095\/biolreprod.106.051813","article-title":"Testosterone stimulates the primary to secondary follicle transition in bovine follicles in Vitro1","volume":"75","author":"Yang","year":"2006","journal-title":"Biol Reprod [Internet]"},{"key":"2019102315500240100_ref47","doi-asserted-by":"crossref","first-page":"4405","DOI":"10.1210\/jc.2016-1589","article-title":"In vitro activation of follicles and fresh tissue auto-transplantation in primary ovarian insufficiency patients","volume":"101","author":"Zhai","year":"2016","journal-title":"J Clin Endocrinol Metab [Internet]"}],"container-title":["Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/humrep\/article-pdf\/34\/10\/1924\/30279252\/dez152.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/humrep\/article-pdf\/34\/10\/1924\/30279252\/dez152.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T15:50:48Z","timestamp":1571845848000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/humrep\/article\/34\/10\/1924\/5583240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":47,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2019,10,8]]},"published-print":{"date-parts":[[2019,10,2]]}},"URL":"https:\/\/doi.org\/10.1093\/humrep\/dez152","relation":{},"ISSN":["0268-1161","1460-2350"],"issn-type":[{"value":"0268-1161","type":"print"},{"value":"1460-2350","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,10]]},"published":{"date-parts":[[2019,10]]}}}