{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T22:55:37Z","timestamp":1783378537215,"version":"3.54.6"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,29]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>STUDY QUESTION<\/jats:title><jats:p>How do manufacturers perform embryotoxicity testing in their quality control programs when validating IVF consumables?<\/jats:p><\/jats:sec><jats:sec><jats:title>SUMMARY ANSWER<\/jats:title><jats:p>The Mouse Embryo Assay (MEA) and Human Sperm Survival Assay (HSSA) used for IVF disposables differed from one manufacturer to another.<\/jats:p><\/jats:sec><jats:sec><jats:title>WHAT IS KNOWN ALREADY<\/jats:title><jats:p>Many components used in IVF laboratories, such as culture media and disposable consumables, may negatively impact human embryonic development.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN, SIZE, DURATION<\/jats:title><jats:p>Through a questionnaire-based survey, the main manufacturers of IVF disposable devices were contacted during the period November to December 2018 to compare the methodology of the MEA and HSSA. We focused on catheters for embryo transfer, catheters for insemination, straws, serological pipettes, culture dishes and puncture needles used in the ART procedures.<\/jats:p><\/jats:sec><jats:sec><jats:title>PARTICIPANTS\/MATERIALS, SETTING, METHODS<\/jats:title><jats:p>We approached the manufacturers of IVF disposables and asked for details about methodology of the MEA and HSSA performed for toxicity testing of their IVF disposable devices. All specific parameters like mouse strains, number of embryos used, culture conditions (media, temperature, atmosphere), extraction protocol, subcontracting, and thresholds were registered and compared between companies.<\/jats:p><\/jats:sec><jats:sec><jats:title>MAIN RESULTS AND THE ROLE OF CHANCE<\/jats:title><jats:p>Twenty-one companies were approached, of which only 11 answered the questionnaire. Significant differences existed in the methodologies and thresholds of the MEA and HSSA used for toxicity testing of IVF disposables. Importantly, some of these parameters could influence the sensitivity of the tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>LIMITATIONS, REASONS FOR CAUTION<\/jats:title><jats:p>Although we approached the main IVF manufacturers, the response rate was relatively low.<\/jats:p><\/jats:sec><jats:sec><jats:title>WIDER IMPLICATIONS OF THE FINDINGS<\/jats:title><jats:p>Our study confirms the high degree of heterogeneity of the embryotoxicity tests performed by manufacturers when validating their IVF disposable devices. Currently, no regulations exist on this issue. Professionals should call for and request standardization and a future higher degree of transparency as regards embryotoxicity testing from supplying companies; moreover, companies should be urged to provide the users clear and precise information about the results of their tests and how testing was performed. Future recommendations are urgently awaited to improve the sensitivity and reproducibility of embryotoxicity assays over time.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY FUNDING\/COMPETING INTEREST(S)<\/jats:title><jats:p>This study did not receive any funding. L.D. declares a competing interest with Patrick Choay SAS.<\/jats:p><\/jats:sec><jats:sec><jats:title>TRIAL REGISTRATION NUMBER<\/jats:title><jats:p>N\/A<\/jats:p><\/jats:sec>","DOI":"10.1093\/humrep\/dez277","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T20:47:09Z","timestamp":1575319629000},"page":"283-292","source":"Crossref","is-referenced-by-count":15,"title":["Embryotoxicity testing of IVF disposables: how do manufacturers test?"],"prefix":"10.1093","volume":"35","author":[{"given":"L","family":"Delaroche","sequence":"first","affiliation":[{"name":"Centre d'AMP, Ramsay Sant\u00e9, H\u00f4pital Priv\u00e9 de Parly 2, 21 rue Moxouris, 78150 Le Chesnay, France"},{"name":"Centre de Biologie M\u00e9dicale, H\u00f4pital Priv\u00e9 de Parly 2, 21 rue Moxouris, 78150 Le Chesnay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Oger","sequence":"first","affiliation":[{"name":"Centre d'AMP, Ramsay Sant\u00e9, H\u00f4pital Priv\u00e9 de Parly 2, 21 rue Moxouris, 78150 Le Chesnay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Genauzeau","sequence":"first","affiliation":[{"name":"Centre d'AMP, Ramsay Sant\u00e9, H\u00f4pital Priv\u00e9 de Parly 2, 21 rue Moxouris, 78150 Le Chesnay, France"},{"name":"Centre de Biologie M\u00e9dicale, H\u00f4pital Priv\u00e9 de Parly 2, 21 rue Moxouris, 78150 Le Chesnay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Meicler","sequence":"first","affiliation":[{"name":"Centre d'AMP, Ramsay Sant\u00e9, H\u00f4pital Priv\u00e9 de Parly 2, 21 rue Moxouris, 78150 Le Chesnay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Lamazou","sequence":"first","affiliation":[{"name":"Centre d'AMP, Ramsay Sant\u00e9, H\u00f4pital Priv\u00e9 de Parly 2, 21 rue Moxouris, 78150 Le Chesnay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Dupont","sequence":"first","affiliation":[{"name":"INSERM \u00e9quipe Lipodystrophies g\u00e9n\u00e9tiques et acquises. 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Res"},{"key":"2020022823094812600_ref3","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/BF01131499","article-title":"Toxicity testing for human in vitro fertilization programs","volume":"2","author":"Ackerman","year":"1985","journal-title":"J In Vitro Fert Embryo Transf"},{"key":"2020022823094812600_ref4","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s10815-016-0856-4","article-title":"Improved detection of mineral oil toxicity using an extended mouse embryo assay","volume":"34","author":"Ainsworth","year":"2017","journal-title":"J Assist Reprod Genet"},{"key":"2020022823094812600_ref5","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1095\/biolreprod.113.113084","article-title":"When stresses collide","volume":"89","author":"Awonuga","year":"2013","journal-title":"Biol Reprod"},{"key":"2020022823094812600_ref6","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0029388","article-title":"Adult body weight is programmed by a 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A"},{"key":"2020022823094812600_ref16","volume-title":"Title 21","author":"FDA","year":"1998"},{"key":"2020022823094812600_ref17","doi-asserted-by":"crossref","first-page":"5880","DOI":"10.1073\/pnas.0308560101","article-title":"Long-term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development, and behavior","volume":"101","author":"Fernandez-Gonzalez","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2020022823094812600_ref18","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1095\/biolreprod41.5.835","article-title":"Mouse zygote development in culture medium without protein in the presence of ethylenediaminetetraacetic acid","volume":"41","author":"Fissore","year":"1989","journal-title":"Biol Reprod"},{"key":"2020022823094812600_ref19","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/S0015-0282(16)55638-6","article-title":"The mouse embryo culture system: improving the sensitivity for use as a quality control assay for human in vitro fertilization","volume":"59","author":"Fleetham","year":"1993","journal-title":"Fertil Steril"},{"key":"2020022823094812600_ref20","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1002\/mrd.1080300310","article-title":"Reduction of embryotoxicity by protein in embryo culture media","volume":"30","author":"Flood","year":"1991","journal-title":"Mol Reprod Dev"},{"key":"2020022823094812600_ref21","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1055\/s-2005-923389","article-title":"Quality control in human in vitro fertilization","volume":"23","author":"Gardner","year":"2005","journal-title":"Semin Reprod Med"},{"key":"2020022823094812600_ref22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s12958-016-0149-x","article-title":"Genetic mouse embryo assay: improving performance and quality testing for assisted reproductive technology (ART) with a functional 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Reprod Genet"},{"key":"2020022823094812600_ref26","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s10815-005-4916-4","article-title":"The rapid detection of cytotoxicity using a modified human sperm survival assay","volume":"22","author":"Iemmolo","year":"2005","journal-title":"J Assist Reprod Genet"},{"key":"2020022823094812600_ref27","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1016\/j.fertnstert.2012.10.046","article-title":"Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice","volume":"99","author":"Khan","year":"2013","journal-title":"Fertil Steril"},{"key":"2020022823094812600_ref28","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1093\/humrep\/dew156","article-title":"Influence of embryo culture medium (G5 and HTF) on pregnancy and perinatal outcome after IVF: a multicenter RCT","volume":"31","author":"Kleijkers","year":"2016","journal-title":"Hum Reprod"},{"key":"2020022823094812600_ref29","first-page":"209","article-title":"A comparative study of two-cell embryos of CBA and F1 mice in a human in vitro fertilization program","volume":"65","author":"Kruger","year":"1984","journal-title":"S Afr Med J"},{"key":"2020022823094812600_ref30","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1071\/RD07161","article-title":"To QC or not to QC: the key to a consistent laboratory?","volume":"20","author":"Lane","year":"2008","journal-title":"Reprod Fertil Dev"},{"key":"2020022823094812600_ref31","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0168-583X(99)00129-9","article-title":"Ion-beam modification of polymeric materials-fundamental principles and applications. 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Up-to-date literature review","volume":"2016","author":"Marianowski","year":"2016","journal-title":"Biomed Res Int"},{"key":"2020022823094812600_ref36","volume-title":"Guidelines on Medical Devices","author":"MEDDEV 2.7\/1 revision 4","year":"2016"},{"key":"2020022823094812600_ref1","volume-title":"Official Journal of 12.07.1993, L169","author":"Medical Device Directive MDD: Council Directive 93\/42\/EEC","year":"14 1993"},{"key":"2020022823094812600_ref37","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.rbmo.2019.05.008","article-title":"Parameters of the mouse embryo assay that affect detection of peroxides in mineral oil","volume":"39","author":"Mestres","year":"2019","journal-title":"Reprod Biomed Online"},{"key":"2020022823094812600_ref38","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1055\/s-0032-1311520","article-title":"Importance of supply integrity for in vitro fertilization and embryo culture","volume":"30","author":"Morbeck","year":"2012","journal-title":"Semin Reprod Med"},{"key":"2020022823094812600_ref39","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/S0015-0282(16)49834-1","article-title":"Factors influencing murine embryo bioassay: effects of proteins, aging of medium, and surgical glove coatings","volume":"46","author":"Naz","year":"1986","journal-title":"Fertil Steril"},{"key":"2020022823094812600_ref40","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.fertnstert.2008.07.011","article-title":"Reprotoxicity of intrauterine insemination and in vitro fertilization-embryo transfer disposables and products: a 4-year survey","volume":"92","author":"Nijs","year":"2009","journal-title":"Fertil Steril"},{"key":"2020022823094812600_ref41","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1139\/y2012-049","article-title":"On the disruption of biochemical and biological assays by chemicals leaching from disposable laboratory plasticware","volume":"90","author":"Olivieri","year":"2012","journal-title":"Can J Physiol Pharmacol"},{"key":"2020022823094812600_ref42","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.fertnstert.2006.11.144","article-title":"Peroxidation of mineral oil used in droplet culture is detrimental to fertilization and embryo development","volume":"88","author":"Otsuki","year":"2007","journal-title":"Fertil Steril"},{"key":"2020022823094812600_ref43","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1016\/j.fertnstert.2008.03.001","article-title":"Damage of embryo development caused by peroxidized mineral oil and its association with albumin in culture","volume":"91","author":"Otsuki","year":"2009","journal-title":"Fertil Steril"},{"key":"2020022823094812600_ref44","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/BF01555437","article-title":"Mouse system quality control is necessary before the use of new material for in vitro fertilization and embryo transfer","volume":"4","author":"Parinaud","year":"1987","journal-title":"J In Vitro Fert Embryo Transf"},{"key":"2020022823094812600_ref45","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/S1472-6483(10)60130-7","article-title":"Toxicity testing of human assisted reproduction devices using the mouse embryo assay","volume":"18","author":"Punt-van der Zalm","year":"2009","journal-title":"Reprod Biomed Online"},{"key":"2020022823094812600_ref46","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/BF01555438","article-title":"In vitro fertilization: fertilization failure due to toxic catheters","volume":"4","author":"Ray","year":"1987","journal-title":"J In Vitro Fert Embryo Transf"},{"key":"2020022823094812600_ref47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/hmg\/ddm280","article-title":"Manipulations of mouse embryos prior to implantation result in aberrant expression of imprinted genes on day 9.5 of 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