{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:49:42Z","timestamp":1788346182817,"version":"build-2803163510"},"reference-count":62,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>To prevent the transfer of embryos affected by monogenic conditions and\/or chromosomal defects, preimplantation genetic testing (PGT) requires trophectoderm biopsy and cryopreservation. In 2\u20136% of biopsies, the diagnosis may be inconclusive due to DNA amplification failure or low-quality results. In these cases, a round of re-warming, re-biopsy, and re-cryopreservation is required to obtain a genetic diagnosis. In other cases, when the IVF centre starts providing PGT and\/or when the patients develop an indication because of multiple failures, miscarriages or the birth of an affected child after IVF, cryopreserved untested embryos may be warmed, biopsied, and then re-vitrified. However, it is still unclear whether multiple manipulations may reduce reproductive outcomes after PGT.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>OBJECTIVE AND RATIONALE<\/jats:title>\n                  <jats:p>This study aimed at conducting a systematic review to investigate the available evidence on the safety of double biopsy and\/or double cryopreservation\u2013warming and provide recommendations in this regard. We performed meta-analyses of the differences in the reproductive outcomes (live birth per embryo transfer [LBR per ET], clinical pregnancy rate per ET [CPR per ET], and miscarriage rate per clinical pregnancy [MR per CP]) in double cryopreservation and single biopsy (CBC) or double biopsy and double cryopreservation (BCBC) flows vs the control single biopsy and single cryopreservation (BC) flow. Cryo-survival rates before ET and gestational and perinatal outcomes were also reported.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>SEARCH METHODS<\/jats:title>\n                  <jats:p>PRISMA guidelines were followed to gather all available information from the literature (PubMed, Scopus, and Embase). We used Medical Subject Headings (MeSH) terms and a list of specific keywords relevant for the study question. We searched for original studies in humans, published in peer-reviewed journals in English up to April 2024. Four independent authors assessed the articles for inclusion. One included paper was retrieved from another source.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>OUTCOMES<\/jats:title>\n                  <jats:p>A total of 4219 records were identified, and 10 studies were included in the meta-analysis. Certainty of evidence level ranged from low to moderate. Both the CBC and BCBC groups showed reduced reproductive outcomes compared to the control (BC). Specifically, live birth rates per embryo transfer were lower in the CBC group (OR: 0.56, 95% CI: 0.38\u20130.81, I2 = 58%; six studies) and the BCBC group (OR: 0.51, 95% CI: 0.34\u20130.77, I2 = 24%; six studies). CPR per ET were also lower in the CBC group (OR: 0.68, 95% CI: 0.51\u20130.92, I2 = 57%; seven studies) and the BCBC group (OR: 0.60, 95% CI: 0.46\u20130.78, I2 = 0%; seven studies). Additionally, MR per CPs were higher in both the CBC group (OR: 1.68, 95% CI: 1.02\u20132.77, I2 = 50%; seven studies) and the BCBC group (OR: 2.08, 95% CI: 1.13\u20133.83, I2 = 28%; seven studies). Cryo-survival as well as gestational and perinatal outcomes were within the expected norms in the studies reporting them.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>WIDER IMPLICATIONS<\/jats:title>\n                  <jats:p>Improved genetic technologies, standardization of laboratory protocols, operators\u2019 proficiency with biopsy and cryopreservation, and continuous monitoring of the performance are essential to minimize inconclusive diagnoses and the putative impact of additional embryo manipulations. Although poorer reproductive outcomes might result from double biopsy and\/or double cryopreservations, these practices may still be worthwhile to avoid transferring affected\/aneuploid blastocysts. Therefore, the risks must be weighed against the potential benefits for each specific couple.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>REGISTRATION NUMBER<\/jats:title>\n                  <jats:p>PROSPERO (ID: CRD42024503678)<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/humupd\/dmae031","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T16:50:54Z","timestamp":1731689454000},"page":"102-115","source":"Crossref","is-referenced-by-count":14,"title":["A systematic review and meta-analysis of double trophectoderm biopsy and\/or cryopreservation in PGT: balancing the need for a diagnosis against the risk of harm"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6452-0636","authenticated-orcid":false,"given":"Letizia","family":"Li Piani","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences and Community Health, Universit\u00e0 degli Studi di Milano , Milan,","place":["Italy"]},{"name":"Brussels IVF, Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel , Brussels,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6405-0283","authenticated-orcid":false,"given":"Pasquale","family":"Petrone","sequence":"additional","affiliation":[{"name":"Brussels IVF, Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel , Brussels,","place":["Belgium"]},{"name":"Gynecologic Unit, Department of Surgical Sciences, University of Rome \u2018TorVergata\u2019 , Rome,","place":["Italy"]},{"name":"IVIRMA Global Research Alliance, Genera, Clinica Valle Giulia , Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariafrancesca","family":"Brutto","sequence":"additional","affiliation":[{"name":"Brussels IVF, Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel , Brussels,","place":["Belgium"]},{"name":"Department of Obstetrics and Gynecology, Agostino Gemelli University , Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anick","family":"De Vos","sequence":"additional","affiliation":[{"name":"Brussels IVF, Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel , Brussels,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9065-1960","authenticated-orcid":false,"given":"Annelore","family":"Van Der Kelen","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Clinical Sciences, Research Group Genetics, Reproduction and Development, Centre for Medical Genetics , Brussels,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0873-3925","authenticated-orcid":false,"given":"Alberto","family":"Vaiarelli","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, Genera, Clinica Valle Giulia , Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rienzi","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, Genera, Clinica Valle Giulia , Rome,","place":["Italy"]},{"name":"Department of Biomolecular Sciences, University of Urbino \u201cCarlo Bo\u201d , Urbino,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5676-0055","authenticated-orcid":false,"given":"Alessandro","family":"Conforti","sequence":"additional","affiliation":[{"name":"Dipartimento di Neuroscienze e Scienze Riproduttive e Odontostomatologiche, Universit\u00e0 di Napoli Federico II , Naples,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5390-9221","authenticated-orcid":false,"given":"Danilo","family":"Cimadomo","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, Genera, Clinica Valle Giulia , Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5020-2888","authenticated-orcid":false,"given":"Willem","family":"Verpoest","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Utrecht University Medical Center, Utrecht University , Utrecht,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,11,15]]},"reference":[{"key":"2025030423084571500_dmae031-B1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1093\/humupd\/dmad001","article-title":"Obstetric, neonatal, and child health outcomes following embryo biopsy for preimplantation genetic testing","volume":"29","author":"Alteri","year":"2023","journal-title":"Hum Reprod Update"},{"key":"2025030423084571500_dmae031-B2","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.rbmo.2020.11.019","article-title":"Multiple cryopreservation-warming cycles, coupled with blastocyst biopsy, negatively affect IVF outcomes","volume":"42","author":"Aluko","year":"2021","journal-title":"Reprod Biomed Online"},{"key":"2025030423084571500_dmae031-B3","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.fertnstert.2022.10.010","article-title":"Reproductive genetics laboratory may impact euploid blastocyst and live birth rates: a comparison of 4 national laboratories\u2019 PGT-A results from vitrified donor oocytes","volume":"119","author":"Bardos","year":"2023","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B4","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/j.fertnstert.2023.02.006","article-title":"Impact of embryo vitrification on children's health, including growth up to two years of age, in comparison with results following a fresh embryo transfer","volume":"119","author":"Belva","year":"2023","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B5","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s12958-023-01140-3","article-title":"Cleavage-stage or blastocyst-stage embryo biopsy has no impact on growth and health in children up to 2 years of age","volume":"21","author":"Belva","year":"2023","journal-title":"Reprod Biol Endocrinol"},{"key":"2025030423084571500_dmae031-B6","doi-asserted-by":"crossref","DOI":"10.1093\/humrep\/deae235","article-title":"Impacts of double biopsy and double vitrification on the clinical outcomes following euploid blastocyst transfer: a systematic review and meta-analysis","author":"Bickendorf","year":"2024","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/jrsm.12","article-title":"A basic introduction to fixed-effect and random-effects models for meta-analysis","volume":"1","author":"Borenstein","year":"2010","journal-title":"Res Synth Methods"},{"key":"2025030423084571500_dmae031-B8","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.fertnstert.2017.09.013","article-title":"Impact of multiple blastocyst biopsy and vitrification-warming procedures on pregnancy outcomes","volume":"108","author":"Bradley","year":"2017","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B9","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1093\/humupd\/dmae012","article-title":"Screening embryos for polygenic disease risk: a review of epidemiological, clinical, and ethical considerations","author":"Capalbo","year":"2024","journal-title":"Hum Reprod Update"},{"key":"2025030423084571500_dmae031-B10","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1016\/j.ajhg.2022.07.009","article-title":"On the reproductive capabilities of aneuploid human preimplantation embryos","volume":"109","author":"Capalbo","year":"2022","journal-title":"Am J Hum Genet"},{"key":"2025030423084571500_dmae031-B11","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1093\/humrep\/dev294","article-title":"Consistent and reproducible outcomes of blastocyst biopsy and aneuploidy screening across different biopsy practitioners: a multicentre study involving 2586 embryo biopsies","volume":"31","author":"Capalbo","year":"2016","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B12","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1093\/humrep\/dey291","article-title":"Associations of blastocyst features, trophectoderm biopsy and other laboratory practice with post-warming behavior and implantation","volume":"33","author":"Cimadomo","year":"2018","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B13","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1093\/humupd\/dmaa019","article-title":"The dawn of the future: 30 years from the first biopsy of a human embryo. The detailed history of an ongoing revolution","volume":"26","author":"Cimadomo","year":"2020","journal-title":"Hum Reprod Update"},{"key":"2025030423084571500_dmae031-B14","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1093\/humupd\/dmad010","article-title":"Opening the black box: why do euploid blastocysts fail to implant? A systematic review and meta-analysis","volume":"29","author":"Cimadomo","year":"2023","journal-title":"Hum Reprod Update"},{"key":"2025030423084571500_dmae031-B15","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1093\/humrep\/dey282","article-title":"Inconclusive chromosomal assessment after blastocyst biopsy: prevalence, causative factors and outcomes after re-biopsy and re-vitrification. A multicenter experience","volume":"33","author":"Cimadomo","year":"2018","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B16","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1093\/humrep\/deac080","article-title":"How slow is too slow? A comprehensive portrait of Day 7 blastocysts and their clinical value standardized through artificial intelligence","volume":"37","author":"Cimadomo","year":"2022","journal-title":"Hum Reprod"},{"issue":"Suppl 1","key":"2025030423084571500_dmae031-B17","first-page":"e1","article-title":"PGDIS position statement on the transfer of mosaic embryos 2019","volume":"39","author":"Cram","year":"2019","journal-title":"Reprod Biomed Online"},{"key":"2025030423084571500_dmae031-B18","doi-asserted-by":"crossref","first-page":"A45","DOI":"10.1530\/REP-20-0082","article-title":"PREIMPLANTATION GENETIC TESTING: clinical experience of preimplantation genetic testing","volume":"160","author":"De Rycke","year":"2020","journal-title":"Reproduction"},{"key":"2025030423084571500_dmae031-B19","doi-asserted-by":"crossref","first-page":"hoac044","DOI":"10.1093\/hropen\/hoac044","article-title":"ESHRE Working Group on Chromosomal Mosaicism\u2014ESHRE survey results and good practice recommendations on managing chromosomal mosaicism","volume":"2022","author":"De Rycke","year":"2022","journal-title":"Hum Reprod Open"},{"key":"2025030423084571500_dmae031-B20","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/s10038-020-0732-z","article-title":"Preimplantation genetic testing with HLA matching: from counseling to birth and beyond","volume":"65","author":"De Rycke","year":"2020","journal-title":"J Hum Genet"},{"key":"2025030423084571500_dmae031-B21","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1111\/jog.15275","article-title":"The preimplantation genetic testing clinical outcomes of biopsy on vitrification-warming embryos: a retrospective study","volume":"48","author":"Ding","year":"2022","journal-title":"J Obstet Gynaecol Res"},{"key":"2025030423084571500_dmae031-B22","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1093\/humupd\/dms016","article-title":"A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos","volume":"18","author":"Edgar","year":"2012","journal-title":"Hum Reprod Update"},{"key":"2025030423084571500_dmae031-B23","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1136\/bmj.315.7109.629","article-title":"Bias in meta-analysis detected by a simple, graphical test","volume":"315","author":"Egger","year":"1997","journal-title":"BMJ"},{"key":"2025030423084571500_dmae031-B24","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.fertnstert.2013.11.004","article-title":"The nature of aneuploidy with increasing age of the female partner: a review of 15,169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening","volume":"101","author":"Franasiak","year":"2014","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B25","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.fertnstert.2017.12.017","article-title":"Next generation sequencing for preimplantation genetic screening improves pregnancy outcomes compared with array comparative genomic hybridization in single thawed euploid embryo transfer cycles","volume":"109","author":"Friedenthal","year":"2018","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B26","doi-asserted-by":"crossref","first-page":"103852","DOI":"10.1016\/j.ejmg.2020.103852","article-title":"Clinical error rates of next generation sequencing and array comparative genomic hybridization with single thawed euploid embryo transfer","volume":"63","author":"Friedenthal","year":"2020","journal-title":"Eur J Med Genet"},{"key":"2025030423084571500_dmae031-B27","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1093\/humrep\/deae040","article-title":"Does recurrent implantation failure exist? Prevalence and outcomes of five consecutive euploid blastocyst transfers in 123 987 patients","author":"Gill","year":"2024","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B28","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1007\/s10815-024-03078-w","article-title":"The impact of a second embryo biopsy for preimplantation genetic testing for monogenic diseases (PGT-M) with inconclusive results on pregnancy potential: results from a matched case-control study","volume":"41","author":"Guarneri","year":"2024","journal-title":"J Assist Reprod Genet"},{"key":"2025030423084571500_dmae031-B29","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.jclinepi.2010.04.026","article-title":"GRADE guidelines: 1. Introduction-GRADE evidence profiles and summary of findings tables","volume":"64","author":"Guyatt","year":"2011","journal-title":"J Clin Epidemiol"},{"key":"2025030423084571500_dmae031-B30","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/344768a0","article-title":"Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification","volume":"344","author":"Handyside","year":"1990","journal-title":"Nature"},{"key":"2025030423084571500_dmae031-B31","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.fertnstert.2021.10.011","article-title":"The concordance rates of an initial trophectoderm biopsy with the rest of the embryo using PGTseq, a targeted next-generation sequencing platform for preimplantation genetic testing-aneuploidy","volume":"117","author":"Kim","year":"2022","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B32","doi-asserted-by":"crossref","first-page":"hoaa020","DOI":"10.1093\/hropen\/hoaa020","article-title":"ESHRE PGT Consortium and SIG Embryology good practice recommendations for polar body and embryo biopsy for PGT","volume":"2020","author":"Kokkali","year":"2020","journal-title":"Hum Reprod Open"},{"key":"2025030423084571500_dmae031-B33","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1002\/ijgo.14355","article-title":"Double vitrification-warming cycles, coupled with blastocyst biopsy, impair live birth but do not affect neonatal outcomes","volume":"160","author":"Li","year":"2023","journal-title":"Int J Gynaecol Obstet"},{"key":"2025030423084571500_dmae031-B34","doi-asserted-by":"publisher","DOI":"10.3791\/59625","article-title":"Human blastocyst biopsy and vitrification","author":"Maggiulli","year":"2019","journal-title":"J Vis Exp"},{"key":"2025030423084571500_dmae031-B35","doi-asserted-by":"crossref","first-page":"103741","DOI":"10.1016\/j.ejmg.2019.103741","article-title":"Clinical outcomes after the transfer of blastocysts characterized as mosaic by high resolution Next Generation Sequencing\u2014further insights","volume":"63","author":"Munne","year":"2020","journal-title":"Eur J Med Genet"},{"key":"2025030423084571500_dmae031-B36","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.1093\/humrep\/dew135","article-title":"Intent to treat analysis of in vitro fertilization and preimplantation genetic screening versus expectant management in patients with recurrent pregnancy loss","volume":"31","author":"Murugappan","year":"2016","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B37","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.fertnstert.2016.11.013","article-title":"High relative deoxyribonucleic acid content of trophectoderm biopsy adversely affects pregnancy outcomes","volume":"107","author":"Neal","year":"2017","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B38","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1007\/s10815-019-01550-6","article-title":"When next-generation sequencing-based preimplantation genetic testing for aneuploidy (PGT-A) yields an inconclusive report: diagnostic results and clinical outcomes after re biopsy","volume":"36","author":"Neal","year":"2019","journal-title":"J Assist Reprod Genet"},{"key":"2025030423084571500_dmae031-B39","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1007\/s10815-023-02875-z","article-title":"Should embryo rebiopsy be considered a regular strategy to increase the number of embryos available for transfer?","volume":"40","author":"Nohales","year":"2023","journal-title":"J Assist Reprod Genet"},{"key":"2025030423084571500_dmae031-B40","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: an updated guideline for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"2025030423084571500_dmae031-B41","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s10815-018-1347-6","article-title":"Minimizing mosaicism: assessing the impact of fertilization method on rate of mosaicism after next-generation sequencing (NGS) preimplantation genetic testing for aneuploidy (PGT-A)","volume":"36","author":"Palmerola","year":"2019","journal-title":"J Assist Reprod Genet"},{"key":"2025030423084571500_dmae031-B42","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1093\/humrep\/dez012","article-title":"Extended in vitro culture of human embryos demonstrates the complex nature of diagnosing chromosomal mosaicism from a single trophectoderm biopsy","volume":"34","author":"Popovic","year":"2019","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B43","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.fertnstert.2023.03.003","article-title":"Indications and management of preimplantation genetic testing for monogenic conditions: a committee opinion","volume":"120","author":"Practice and Genetic Counseling Professional Group of the American Society for Reproductive Medicine","year":"2023","journal-title":". Fertil Steril"},{"key":"2025030423084571500_dmae031-B44","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1016\/j.fertnstert.2022.02.016","article-title":"Comprehensive guidance for human embryology, andrology, and endocrinology laboratories: management and operations: a committee opinion","volume":"117","author":"Practice Committees of the American Society for Reproductive Medicine (ASRM) and the Society for Reproductive Biologists and Technologists (SRBT)","year":"2022","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B45","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/s10815-020-01739-0","article-title":"The impact of age beyond ploidy: outcome data from 8175 euploid single embryo transfers","volume":"37","author":"Reig","year":"2020","journal-title":"J Assist Reprod Genet"},{"key":"2025030423084571500_dmae031-B46","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1093\/humrep\/dew311","article-title":"PGS for recurrent pregnancy loss: still an open question","volume":"32","author":"Rienzi","year":"2017","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B47","first-page":"139","article-title":"Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance","volume":"23","author":"Rienzi","year":"2017","journal-title":"Hum Reprod Update"},{"key":"2025030423084571500_dmae031-B48","doi-asserted-by":"crossref","first-page":"2493","DOI":"10.1007\/s10815-019-01609-4","article-title":"Preimplantation genetic testing for aneuploidy improves clinical, gestational, and neonatal outcomes in advanced maternal age patients without compromising cumulative live-birth rate","volume":"36","author":"Sacchi","year":"2019","journal-title":"J Assist Reprod Genet"},{"key":"2025030423084571500_dmae031-B49","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1093\/humrep\/dez289","article-title":"Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure","volume":"35","author":"Sato","year":"2020","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B50","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.fertnstert.2013.07.004","article-title":"Selecting the optimal time to perform biopsy for preimplantation genetic testing","volume":"100","author":"Scott","year":"2013","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B51","doi-asserted-by":"crossref","first-page":"hoad010","DOI":"10.1093\/hropen\/hoad010","article-title":"ESHRE PGT Consortium data collection XXI: PGT analyses in 2018","volume":"2023","author":"Spinella","year":"2023","journal-title":"Hum Reprod Open"},{"key":"2025030423084571500_dmae031-B52","doi-asserted-by":"crossref","first-page":"i4919","DOI":"10.1136\/bmj.i4919","article-title":"ROBINS-I: a tool for assessing risk of bias in non-randomised studies of interventions","volume":"355","author":"Sterne","year":"2016","journal-title":"BMJ"},{"key":"2025030423084571500_dmae031-B53","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1136\/bmj.320.7249.1574","article-title":"Empirical assessment of effect of publication bias on meta-analyses","volume":"320","author":"Sutton","year":"2000","journal-title":"BMJ"},{"key":"2025030423084571500_dmae031-B54","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1146\/annurev-genom-091416-035508","article-title":"Advances in preimplantation genetic testing for monogenic disease and aneuploidy","volume":"18","author":"Treff","year":"2017","journal-title":"Annu Rev Genomics Hum Genet"},{"key":"2025030423084571500_dmae031-B55","doi-asserted-by":"crossref","first-page":"103619","DOI":"10.1016\/j.rbmo.2023.103619","article-title":"Multiple embryo manipulations in PGT-A cycles may result in inferior clinical outcomes","volume":"48","author":"Vanderhoff","year":"2024","journal-title":"Reprod Biomed Online"},{"key":"2025030423084571500_dmae031-B56","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.rbmo.2011.06.006","article-title":"PGD and HLA matching: not a quick fix","volume":"23","author":"Verpoest","year":"2011","journal-title":"Reprod Biomed Online"},{"key":"2025030423084571500_dmae031-B57","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/j.fertnstert.2023.07.022","article-title":"Chromosomal, gestational, and neonatal outcomes of embryos classified as a mosaic by preimplantation genetic testing for aneuploidy","volume":"120","author":"Viotti","year":"2023","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B58","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.fertnstert.2023.03.001","article-title":"The effect of recryopreservation on embryo viability and outcomes of in vitro fertilization: a systematic review and meta-analysis","volume":"120","author":"Wang","year":"2023","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B59","doi-asserted-by":"crossref","first-page":"e0267652","DOI":"10.1371\/journal.pone.0267652","article-title":"Freeze all-first versus biopsy-first: A retrospective analysis of frozen blastocyst transfer cycles with preimplantation genetic testing for aneuploidy","volume":"17","author":"Yu","year":"2022","journal-title":"PLoS One"},{"key":"2025030423084571500_dmae031-B60","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1093\/humrep\/dex234","article-title":"The International Glossary on Infertility and Fertility Care, 2017","volume":"32","author":"Zegers-Hochschild","year":"2017","journal-title":"Hum Reprod"},{"key":"2025030423084571500_dmae031-B61","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.fertnstert.2017.06.005","article-title":"The International Glossary on Infertility and Fertility Care, 2017","volume":"108","author":"Zegers-Hochschild","year":"2017","journal-title":"Fertil Steril"},{"key":"2025030423084571500_dmae031-B62","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.fertnstert.2015.10.003","article-title":"Development and validation of concurrent preimplantation genetic diagnosis for single gene disorders and comprehensive chromosomal aneuploidy screening without whole genome amplification","volume":"105","author":"Zimmerman","year":"2016","journal-title":"Fertil Steril"}],"container-title":["Human Reproduction Update"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/humupd\/article-pdf\/31\/2\/102\/60686310\/dmae031.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/humupd\/article-pdf\/31\/2\/102\/60686310\/dmae031.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T23:09:08Z","timestamp":1741129748000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/humupd\/article\/31\/2\/102\/7901238"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,15]]},"references-count":62,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,11,15]]},"published-print":{"date-parts":[[2025,3,1]]}},"URL":"https:\/\/doi.org\/10.1093\/humupd\/dmae031","relation":{},"ISSN":["1355-4786","1460-2369"],"issn-type":[{"value":"1355-4786","type":"print"},{"value":"1460-2369","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,3]]},"published":{"date-parts":[[2024,11,15]]}}}