{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:25:13Z","timestamp":1792794313761,"version":"4.5.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"University Medical Center Rostock","award":["889007"],"award-info":[{"award-number":["889007"]}],"id":[{"id":"https:\/\/ror.org\/04dm1cm79","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["331003022"],"award-info":[{"award-number":["331003022"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Crohn\u2019s disease (CD) itself is discussed as risk factors for anastomotic leakage (AL) after ileocecal resection (ICR). In order to gain insight into the mechanisms of anastomotic healing (AH) in Crohn\u2019s ileitis, we performed ICR in a mouse model of spontaneous ileitis. Additionally, we investigated the impact of anti-\u03b14\u03b27 integrin antibody on AH.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>SAMP1\/YitFc (SAMP1) and parental control (AKR) mice were subjected to ICR. Mice received an anti-\u03b14\u03b27 integrin antibody or isotype control (25\u2009mg\/kg). Functional (bursting pressure), macroscopic (healing score), and matrix-related parameters (hydroxyproline, collagenase activity, and histology) of AH were assessed at day 5. Local and systemic cytokine messenger RNA expression and lipocalin-2 in plasma and stool were measured. Effects of \u03b14\u03b27 integrin blockade on anastomotic cell infiltration were assessed by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SAMP1 mice exhibited a 5-fold higher rate of microabscess formation in the anastomoses and worse macroscopical healing. Functional healing and matrix parameters, as well as leak rate, were similar to AKR control mice. Despite an increase in systemic inflammation, a significant drop in T helper 1 cytokine expression was observed in the mesenteric lymph nodes of SAMP1 mice. Flow cytometric analyses revealed a higher proportion of mesenchymal cells and myeloid suppressor cells. Wound macrophages remained unchanged in the anastomotic tissue of anti-\u03b14\u03b27 integrin antibody\u2013treated SAMP1 mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Crohn\u2019s-like ileitis mice were at higher risk for abscess formation. Anti-\u03b14\u03b27 integrin treatment resulted in cellular changes that did not impair functional anastomotic healing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ibd\/izaf223","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T11:34:03Z","timestamp":1757417643000},"page":"3385-3397","source":"Crossref","is-referenced-by-count":1,"title":["Perioperative anti-\u03b14\u03b27 Integrin Blockade Alters Wound Cell Infiltrate but Not the Functional Outcome of Ileocecal Anastomoses in Mice With Crohn\u2019s-like Ileitis"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8354-6970","authenticated-orcid":false,"given":"Peggy","family":"Berlin","sequence":"first","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutritional Medicine, Department of Internal Medicine, Rostock University Medical Center , Rostock, 18057,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabel","family":"Fischer","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutritional Medicine, Department of Internal Medicine, Rostock University Medical Center , Rostock, 18057,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Bergmann-Ewert","sequence":"additional","affiliation":[{"name":"Core Facility for Cell Sorting and Cell Analysis, Rostock University Medical Center , Rostock, 18057,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Kordt","sequence":"additional","affiliation":[{"name":"Core Facility for Cell Sorting and Cell Analysis, Rostock University Medical Center , Rostock, 18057,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens","family":"Schafmayer","sequence":"additional","affiliation":[{"name":"Division of General, Visceral, Thoracic, Vascular and Transplant Surgery, Department of Operative Medicine, Rostock University Medical Center , Rostock, 18057,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Lamprecht","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutritional Medicine, Department of Internal Medicine, Rostock University Medical Center , Rostock, 18057,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Reiner","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutritional Medicine, Department of Internal Medicine, Rostock University Medical Center , Rostock, 18057,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria B","family":"Witte","sequence":"additional","affiliation":[{"name":"Division of General, Visceral, Thoracic, Vascular and Transplant Surgery, Department of Operative Medicine, Rostock University Medical Center , Rostock, 18057,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"2025120914524286000_izaf223-B1","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1007\/s00384-021-03930-w","article-title":"Crohn\u2019s disease: a population-based study of surgery in the age of biological therapy","volume":"36","author":"St\u00f6ss","year":"2021","journal-title":"Int J Colorectal Dis"},{"key":"2025120914524286000_izaf223-B2","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1016\/j.cgh.2020.10.039","article-title":"Contemporary risk of surgery in patients with ulcerative Colitis and Crohn\u2019s disease: a meta-analysis of population-based cohorts","volume":"19","author":"Tsai","year":"2021","journal-title":"Clin Gastroenterol Hepatol"},{"key":"2025120914524286000_izaf223-B3","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1093\/ecco-jcc\/jjae089","article-title":"ECCO guidelines on therapeutics in Crohn\u2019s disease: surgical treatment","volume":"18","author":"Adamina","year":"2024","journal-title":"J Crohns Colitis"},{"key":"2025120914524286000_izaf223-B4","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.1136\/gutjnl-2018-317539","article-title":"Cost-effectiveness of laparoscopic ileocaecal resection versus infliximab treatment of terminal ileitis in Crohn\u2019s disease: the LIR! 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