{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:28:49Z","timestamp":1790839729559,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T00:00:00Z","timestamp":1574812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"French Institute of Health INSERM"},{"name":"Association Fran\u00e7ois Aupetit"},{"name":"Universit\u00e9 de Paris"},{"name":"Investissements d\u2019Avenir programme","award":["ANR-11-IDEX-0005-02"],"award-info":[{"award-number":["ANR-11-IDEX-0005-02"]}]},{"name":"Sorbonne Paris Cit\u00e9, Laboratoire d\u2019excellence INFLAMEX"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Fistulizing anoperineal lesions (FAPLs) are common and severe complications of Crohn\u2019s disease (CD), exposing patients to the risk of anal sphincter alteration and permanent stoma. Due to the limited efficacy of current treatments, identifying new local therapies is mandatory. However, testing new treatments is currently limited because no relevant preclinical model of Crohn\u2019s-like FAPL is available. Thus, a reliable and reproducible experimental model of FAPLs is needed to assess new therapeutic strategies.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Twenty-one rats received a rectal enema of 2,4,6-trinitrobenzensulfonic acid (TNBS) to induce proctitis. Seven days later, a transsphincteric fistula tract was created with a surgical thread, instilled with TNBS twice a week until its removal at day 7 (group 1), day 14 (group 2), or day 28 (group 3). In each rat, pelvic MRI was performed just before and 7 days after thread removal. Rats were sacrificed 7 days after thread removal for pathological assessment of the fistula tract.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The optimal preclinical model was obtained in group 3. In this group, 7 days after thread removal, all animals (9 of 9) had a persistent fistula tract visible on MRI with T2-hypersignal (normalized T2 signal intensity: 2.36 \u00b1 0.39 arbitrary units [a.u.] [2.08\u20132.81]) and elevation of the apparent diffusion coefficient (1.33 \u00b1 0.16 10-3 millimeter squared per seconds [1.18\u20131.49]). The pathological examination of the fistula tract revealed acute and chronic inflammation, granulations, fibrosis, epithelialization, and proctitis in the adjacent rectum.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This reproducible preclinical model could be used to assess the effectiveness of innovative treatments in perianal fistulizing CD.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ibd\/izz288","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T16:40:35Z","timestamp":1574872835000},"page":"687-696","source":"Crossref","is-referenced-by-count":22,"title":["Preclinical Model of Perianal Fistulizing Crohn\u2019s Disease"],"prefix":"10.1093","volume":"26","author":[{"given":"Meredith","family":"Flacs","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, Assistance Publique H\u00f4pitaux de Paris, H\u00f4pital Beaujon, Clichy la Garenne, Clichy Cedex, France"},{"name":"Universit\u00e9 de Paris, Centre de Recherche sur l\u2019Inflammation, INSERM, CNRS, Team Gut Inflammation, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Collard","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, Assistance Publique H\u00f4pitaux de Paris, H\u00f4pital Beaujon, Clichy la Garenne, Clichy Cedex, France"},{"name":"Universit\u00e9 de Paris, Centre de Recherche sur l\u2019Inflammation, INSERM, CNRS, Team Gut Inflammation, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Doblas","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris, Centre de Recherche sur l\u2019Inflammation, INSERM, CNRS, Laboratory of Imaging Biomarkers, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magaly","family":"Zappa","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris, Centre de Recherche sur l\u2019Inflammation, INSERM, CNRS, Laboratory of Imaging Biomarkers, Paris, France"},{"name":"Department of Radiology, Assistance Publique H\u00f4pitaux de Paris, H\u00f4pital Beaujon, Clichy la Garenne, Clichy Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Cazals-Hatem","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris, Centre de Recherche sur l\u2019Inflammation, INSERM, CNRS, Team Gut Inflammation, Paris, France"},{"name":"Department of Pathology, Assistance Publique H\u00f4pitaux de Paris, H\u00f4pital Beaujon, Clichy la Garenne, Clichy Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9on","family":"Maggiori","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, Assistance Publique H\u00f4pitaux de Paris, H\u00f4pital Beaujon, Clichy la Garenne, Clichy Cedex, France"},{"name":"Universit\u00e9 de Paris, Centre de Recherche sur l\u2019Inflammation, INSERM, CNRS, Team Gut Inflammation, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Panis","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, Assistance Publique H\u00f4pitaux de Paris, H\u00f4pital Beaujon, Clichy la Garenne, Clichy Cedex, France"},{"name":"Universit\u00e9 de Paris, Centre de Recherche sur l\u2019Inflammation, INSERM, CNRS, Team Gut Inflammation, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Treton","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris, Centre de Recherche sur l\u2019Inflammation, INSERM, CNRS, Team Gut Inflammation, Paris, France"},{"name":"Department of Gastroenterology, Assistance Publique H\u00f4pitaux de Paris, H\u00f4pital 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