{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T19:27:53Z","timestamp":1778527673384,"version":"3.51.4"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,9,5]],"date-time":"2020-09-05T00:00:00Z","timestamp":1599264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p\/>\n               <jats:sec>\n                  <jats:title>OBJECTIVES<\/jats:title>\n                  <jats:p>It is unclear whether the movement and function of the regenerated cilia on collagen-conjugated artificial trachea are the same as those of normal cilia. This study assessed the ciliary beat frequency (CBF) and ciliary transport functions (CTFs) of regenerated cilia in a canine model.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>A tracheal defect introduced into the anterior portion of the cervical trachea of an adult beagle dog was covered with a collagen-conjugated prosthesis. Two months later, the trachea was harvested along the long axis, both from normal and regenerated regions. The cilia were stained with isothiocyanate-conjugated wheat germ agglutinin, and their movement was monitored with a high-speed camera to analyse CBF and CTF. Four samples each were obtained from the regenerated and normal regions for CBF analysis and 7 samples each were obtained for CTF analysis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>The wheat germ agglutinin-stained cells showed well-regulated beats in both the regenerated and normal regions of the trachea. Mean CBF in the regenerated and normal regions did not differ significantly (7.11\u2009\u00b1\u20090.41 vs 7.14\u2009\u00b1\u20091.09\u2009Hz; P\u2009=\u2009981). By contrast, CTF was significantly lower in the regenerated region than in the normal region (30.0\u2009\u00b1\u20096.62 vs 7.43\u2009\u00b1\u20090.58\u2009\u03bcm\/s; P\u2009=\u20090.005).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>Mean CBF in the regenerated and normal regions did not differ significantly at 2\u2009months. The CTF in the regenerated region recovered partially but remained lower than those in the normal region. Methods are needed to improve the CTF of regenerated cilia.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/icvts\/ivaa167","type":"journal-article","created":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T11:09:07Z","timestamp":1595675347000},"page":"644-649","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of regenerated tracheal cilia function on a collagen-conjugated scaffold in a canine model"],"prefix":"10.1093","volume":"31","author":[{"given":"Yuichiro","family":"Ueda","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan"},{"name":"Department of General Thoracic Surgery, Breast and Pediatric Surgery, Fukuoka University School of Medicine, Fukuoka, Japan"},{"name":"Department of Organ and Tissue Reconstruction, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, 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