{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:14:49Z","timestamp":1740136489943,"version":"3.37.3"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T00:00:00Z","timestamp":1636070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Japan Society for the Promotion of Science KAKENHI Grant-in-Aid for Scientific Research","award":["JP15K10278"],"award-info":[{"award-number":["JP15K10278"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p\/>\n               <jats:sec>\n                  <jats:title>OBJECTIVES<\/jats:title>\n                  <jats:p>Cell therapies, such as stem cell suspension injection, are used to treat bronchopleural fistula. Although it is safe and effective, injected cells cannot remain within the bronchioles of the fistula due to cell leakage into the thoracic cavity. Here, we inserted a \u2018bio plug\u2019 into the fistula, produced using cells and a bio-3D printer, to examine the effectiveness of bio plugs for the closure of bronchopleural fistulas, the optimal cell source and the closure mechanism.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>Bio plugs were made with mesenchymal stem (stromal) cells derived from bone marrow (MSCBM), fibroblasts and rat lung micro-vessel endothelial cells using a bio-3D printer with different cell mixing ratios. Six groups, according to the presence or absence and the type of bio plugs, were compared. The plugs were inserted into the bronchi of F344 rats. The obstruction ratio and histological and immunohistochemical findings were evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>MSCBM+ rat lung micro-vessel endothelial cell group exhibited a higher obstruction ratio among all groups excluding the MSCBM group (P\u2009=\u20090.039). This group had fibrosis and CD31-positive cells and fewer CD68-positive cells than MSCBM and MSCBM+ fibroblast groups.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>Bio plugs with mixed cells, including stem cells, contribute to bronchial closure in the current experimental setting. Endothelial cells effectively maintain the structure in this model. Although bronchial closure for bronchopleural fistula could not be described as clinical conditions were not reproduced, we collected essential data on bronchial closure; however, further experiments are warranted.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/icvts\/ivab306","type":"journal-article","created":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T18:44:36Z","timestamp":1634582676000},"page":"660-667","source":"Crossref","is-referenced-by-count":3,"title":["Successful use of bio plugs for delayed bronchial closure after pneumonectomy in experimental settings"],"prefix":"10.1093","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8674-4403","authenticated-orcid":false,"given":"Masaaki","family":"Moriyama","sequence":"first","affiliation":[{"name":"Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan"},{"name":"Medical-Engineering Hybrid Professional Development Program, Nagasaki University, Nagasaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4344-2322","authenticated-orcid":false,"given":"Keitaro","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan"},{"name":"Medical-Engineering Hybrid Professional Development Program, Nagasaki University, Nagasaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1702-9021","authenticated-orcid":false,"given":"Daisuke","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan"},{"name":"Medical-Engineering Hybrid Professional Development Program, Nagasaki University, Nagasaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryusuke","family":"Machino","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan"},{"name":"Medical-Engineering Hybrid Professional Development Program, Nagasaki University, Nagasaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8419-1478","authenticated-orcid":false,"given":"Tomoshi","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7324-8862","authenticated-orcid":false,"given":"Koichi","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Biomedical Engineering Faculty of Medicine, Saga University, Saga, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Nagayasu","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan"},{"name":"Medical-Engineering Hybrid Professional Development Program, Nagasaki University, Nagasaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,11,5]]},"reference":[{"key":"2022040109595807300_ivab306-B1","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/S1010-7940(00)00541-8","article-title":"Analysis of risk factors in bronchopleural fistula after pulmonary resection for primary lung cancer","volume":"18","author":"Sonobe","year":"2000","journal-title":"Eur J Cardiothorac Surg"},{"key":"2022040109595807300_ivab306-B2","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.athoracsur.2013.10.032","article-title":"Stem cell transplantation effectively occludes bronchopleural fistula in an animal model","volume":"97","author":"Petrella","year":"2014","journal-title":"Ann Thorac Surg"},{"key":"2022040109595807300_ivab306-B3","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1016\/S0022-5223(19)34643-4","article-title":"Bronchopleural fistulas associated with lung cancer operations. Univariate and multivariate analysis of risk factors, management, and outcome","volume":"104","author":"Asamura","year":"1992","journal-title":"J Thorac Cardiovasc Surg"},{"key":"2022040109595807300_ivab306-B4","doi-asserted-by":"crossref","first-page":"3955","DOI":"10.1378\/chest.128.6.3955","article-title":"Bronchopleural fistulas: an overview of the problem with special focus on endoscopic management","volume":"128","author":"Lois","year":"2005","journal-title":"Chest"},{"key":"2022040109595807300_ivab306-B5","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1053\/j.semtcvs.2017.10.003","article-title":"Bronchopleural fistula after pneumonectomy: risk factors and management, focusing on open-window thoracostomy","volume":"30","author":"Mazzella","year":"2018","journal-title":"Semin Thorac Cardiovasc Surg"},{"key":"2022040109595807300_ivab306-B6","doi-asserted-by":"crossref","first-page":"423","DOI":"10.5761\/atcs.oa.13-00040","article-title":"Endobronchial closure of a bronchopleural fistula using a fibrin glue-coated collagen patch and fibrin glue","volume":"19","author":"Ishikawa","year":"2013","journal-title":"Ann Thorac Cardiovasc Surg"},{"key":"2022040109595807300_ivab306-B7","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1177\/1753465816664862","article-title":"A simple method of bronchial occlusion with silicone spigots (Endobronchial Watanabe Spigot; EWS(R)) using a curette","volume":"10","author":"Morikawa","year":"2016","journal-title":"Ther Adv Respir Dis"},{"key":"2022040109595807300_ivab306-B8","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1136\/thx.2007.083857","article-title":"A new bronchoscopic treatment of tracheomediastinal fistula using autologous adipose-derived stem cells","volume":"63","author":"Alvarez","year":"2008","journal-title":"Thorax"},{"key":"2022040109595807300_ivab306-B9","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jcyt.2015.10.003","article-title":"Novel bronchoscopic treatment for bronchopleural fistula using adipose-derived stromal cells","volume":"18","author":"Diaz-Agero Alvarez","year":"2016","journal-title":"Cytotherapy"},{"key":"2022040109595807300_ivab306-B10","doi-asserted-by":"crossref","first-page":"H1320","DOI":"10.1152\/ajpheart.01330.2008","article-title":"Impact of anti-apoptotic and anti-oxidative effects of bone marrow mesenchymal stem cells with transient overexpression of heme oxygenase-1 on myocardial ischemia","volume":"298","author":"Tsubokawa","year":"2010","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"2022040109595807300_ivab306-B11","doi-asserted-by":"crossref","first-page":"H2670","DOI":"10.1152\/ajpheart.01071.2003","article-title":"Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis","volume":"287","author":"Nagaya","year":"2004","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"2022040109595807300_ivab306-B12","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1093\/icvts\/ivx444","article-title":"Scaffold-free trachea regeneration by tissue engineering with bio-3D printing","volume":"26","author":"Taniguchi","year":"2018","journal-title":"Interact CardioVasc Thorac Surg"},{"key":"2022040109595807300_ivab306-B13","first-page":"57","article-title":"Culture methods of cell types obtained from human skin","volume":"22","author":"Inoie","year":"2015","journal-title":"Organ Biol"},{"key":"2022040109595807300_ivab306-B14","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1089\/ten.teb.2016.0322","article-title":"Principles of the Kenzan method for robotic cell spheroid-based three-dimensional bioprinting","volume":"23","author":"Moldovan","year":"2017","journal-title":"Tissue Eng Part B Rev"},{"key":"2022040109595807300_ivab306-B15","doi-asserted-by":"crossref","first-page":"e0211339","DOI":"10.1371\/journal.pone.0211339","article-title":"Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing","volume":"14","author":"Takeoka","year":"2019","journal-title":"PLoS One"},{"key":"2022040109595807300_ivab306-B16","doi-asserted-by":"crossref","first-page":"e1800983","DOI":"10.1002\/adhm.201800983","article-title":"Replacement of rat tracheas by layered, trachea-like, scaffold-free structures of human cells using a Bio-3D printing system","volume":"8","author":"Machino","year":"2019","journal-title":"Adv Healthc Mater"},{"key":"2022040109595807300_ivab306-B17","doi-asserted-by":"crossref","first-page":"101321","DOI":"10.1016\/j.tice.2019.101321","article-title":"Human lung microvascular endothelial cells as potential alternatives to human umbilical vein endothelial cells in bio-3D-printed trachea-like structures","volume":"63","author":"Taniguchi","year":"2020","journal-title":"Tissue Cell"},{"key":"2022040109595807300_ivab306-B18","doi-asserted-by":"crossref","first-page":"e129","DOI":"10.1016\/j.jtcvs.2017.01.030","article-title":"The American Association for Thoracic Surgery consensus guidelines for the management of empyema","volume":"153","author":"Shen","year":"2017","journal-title":"J Thorac Cardiovasc Surg"},{"key":"2022040109595807300_ivab306-B19","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.jtcvs.2014.10.052","article-title":"Vacuum-assisted closure device for the management of infected postpneumonectomy chest cavities","volume":"149","author":"Perentes","year":"2015","journal-title":"J Thorac Cardiovasc Surg"},{"key":"2022040109595807300_ivab306-B20","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.5966\/sctm.2016-0078","article-title":"Closure of a recurrent bronchopleural fistula using a matrix seeded with patient-derived mesenchymal stem cells","volume":"5","author":"Aho","year":"2016","journal-title":"Stem Cells Transl Med"},{"key":"2022040109595807300_ivab306-B21","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1038\/s41467-019-10107-1","article-title":"Development of an immunodeficient pig model allowing long-term accommodation of artificial human vascular tubes","volume":"10","author":"Itoh","year":"2019","journal-title":"Nat Commun"},{"key":"2022040109595807300_ivab306-B22","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1089\/scd.2015.0049","article-title":"Role of endothelial progenitor cells in maintaining stemness and enhancing differentiation of mesenchymal stem cells by indirect cell-cell interaction","volume":"25","author":"Wen","year":"2016","journal-title":"Stem Cells Dev"},{"key":"2022040109595807300_ivab306-B23","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s11748-018-1022-y","article-title":"Risk assessments for broncho-pleural fistula and respiratory failure after lung cancer surgery by National Clinical Database Japan","volume":"67","author":"Endo","year":"2019","journal-title":"Gen Thorac Cardiovasc Surg"},{"key":"2022040109595807300_ivab306-B24","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.5966\/sctm.2015-0285","article-title":"Mesenchymal stem cell-derived exosomes promote fracture healing in a mouse model","volume":"5","author":"Furuta","year":"2016","journal-title":"Stem Cells Transl Med"},{"key":"2022040109595807300_ivab306-B25","doi-asserted-by":"crossref","first-page":"1232810","DOI":"10.1155\/2019\/1232810","article-title":"Paracrine proangiogenic function of human bone marrow-derived mesenchymal stem cells is not affected by chronic kidney disease","volume":"2019","author":"van Rhijn-Brouwer","year":"2019","journal-title":"Stem Cells Int"}],"container-title":["Interactive CardioVascular and Thoracic Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/icvts\/article-pdf\/34\/4\/660\/43219292\/ivab306.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/icvts\/article-pdf\/34\/4\/660\/43219292\/ivab306.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T10:06:42Z","timestamp":1648807602000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/icvts\/article\/34\/4\/660\/6420793"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,5]]},"references-count":25,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,11,5]]},"published-print":{"date-parts":[[2022,3,31]]}},"URL":"https:\/\/doi.org\/10.1093\/icvts\/ivab306","relation":{},"ISSN":["1569-9285"],"issn-type":[{"type":"electronic","value":"1569-9285"}],"subject":[],"published-other":{"date-parts":[[2022,4,1]]},"published":{"date-parts":[[2021,11,5]]}}}