{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T19:32:30Z","timestamp":1774985550978,"version":"3.50.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T00:00:00Z","timestamp":1611964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,18]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Several lines of evidence suggest the abnormalities of protein kinase C (PKC) signaling system in mood disorders and suicide based primarily on the studies of PKC and its isozymes in the platelets and postmortem brain of depressed and suicidal subjects. In this study, we examined the role of PKC isozymes in depression and suicide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We determined the protein and mRNA expression of various PKC isozymes in the prefrontal cortical region (Brodmann area 9) in 24 normal control subjects, 24 depressed suicide (DS) subjects, and 12 depressed nonsuicide (DNS) subjects. The levels of mRNA in the prefrontal cortex were determined by quantitative real-time reverse transcription PCR, and the protein expression was determined by western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed a significant decrease in mRNA expression of PKC\u03b1, PKC\u03b2I, PKC\u03b4, and PKC\u03b5 and decreased protein expression in either the membrane or the cytosol fraction of PKC isozymes PKC\u03b1, PKC\u03b2I, PKC\u03b2II, and PKC\u03b4 in DS and DNS subjects compared with normal control subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The current study provides detailed evidence of specific dysregulation of certain PKC isozymes in the postmortem brain of DS and DNS subjects and further supports earlier evidence for the role of PKC in the platelets and brain of the adult and teenage depressed and suicidal population. This comprehensive study may lead to further knowledge of the involvement of PKC in the pathophysiology of depression and suicide.<\/jats:p><\/jats:sec>","DOI":"10.1093\/ijnp\/pyab003","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T18:02:52Z","timestamp":1611856972000},"page":"400-408","source":"Crossref","is-referenced-by-count":19,"title":["Dysregulation of Protein Kinase C in Adult Depression and Suicide: Evidence From Postmortem Brain Studies"],"prefix":"10.1093","volume":"24","author":[{"given":"Ghanshyam N","family":"Pandey","sequence":"first","affiliation":[{"name":"University of Illinois at Chicago, Department of Psychiatry, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuradha","family":"Sharma","sequence":"additional","affiliation":[{"name":"University of Illinois at Chicago, Department of Psychiatry, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hooriyah S","family":"Rizavi","sequence":"additional","affiliation":[{"name":"University of Illinois at Chicago, Department of Psychiatry, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinguo","family":"Ren","sequence":"additional","affiliation":[{"name":"University of Illinois at Chicago, Department of Psychiatry, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,1,30]]},"reference":[{"key":"2021051811182088100_CIT0001","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s12035-011-8210-4","article-title":"A role for the PKC signaling system in the pathophysiology and treatment of mood disorders: involvement of a functional imbalance?","volume":"44","author":"Abrial","year":"2011","journal-title":"Mol Neurobiol"},{"key":"2021051811182088100_CIT0002","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.pnpbp.2012.11.015","article-title":"Protein kinase C regulates mood-related behaviors and adult hippocampal cell proliferation in rats","volume":"43","author":"Abrial","year":"2013","journal-title":"Prog Neuropsychopharmacol Biol Psychiatry"},{"key":"2021051811182088100_CIT0003","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1038\/sj.npp.1300505","article-title":"Decreased serotonin 5-HT2A receptor-stimulated phosphoinositide signaling in fibroblasts from melancholic depressed patients","volume":"29","author":"Akin","year":"2004","journal-title":"Neuropsychopharmacology"},{"key":"2021051811182088100_CIT0004","doi-asserted-by":"crossref","first-page":"20120535","DOI":"10.1098\/rstb.2012.0535","article-title":"The neurobiology of depression\u2013revisiting the serotonin hypothesis. 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