{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T15:51:57Z","timestamp":1784562717770,"version":"3.55.0"},"reference-count":86,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":32,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"National Institute of Health","award":["75N93019C00070"],"award-info":[{"award-number":["75N93019C00070"]}]},{"name":"National Institute of Health","award":["R01AI177653"],"award-info":[{"award-number":["R01AI177653"]}]},{"name":"National Institute of Health","award":["U19AI135990"],"award-info":[{"award-number":["U19AI135990"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Macrophages are important sites of bacterial replication and host immune responses during Mycobacterium tuberculosis (Mtb) infection with distinct roles for alveolar macrophages (AMs) early in infection and monocyte-derived macrophages (MDMs) later in disease. Here, we leverage data from human and mouse models to perform a cross-species analysis of macrophage responses to Mtb. Overall, we find that both subsets of human and murine macrophages mount a strong interferon response to Mtb infection. However, AMs across both species do not generate as strong a pro-inflammatory response as human MDMs or murine bone marrow-derived macrophages (BMDMs), as characterized by TNFA signaling and inflammatory response pathways. Interestingly, AMs from mice that were previously vaccinated with BCG (scBCG) or from a model of contained TB (coMtb) had more similar responses to human AMs than control mice. We also identify species-specific pathways altered by infection differently in mouse and human macrophages, including cholesterol homeostasis. Lastly, to investigate downstream effects of the macrophage interferon responses, we examine expression of interleukin (IL)-10, an immunosuppressive cytokine induced by Type I Interferons, and c-Maf, a transcription factor required for myeloid IL-10 expression. We find that c-Maf and IL-10 have significantly lower expression in AMs compared to MDMs in both humans and mice, suggesting one possible mechanism by which AMs mount a stronger interferon response following Mtb infection. Overall, these results highlight the dynamics of innate myeloid responses throughout Mtb infection and the benefit of a combined analysis across species to reveal conserved and unique responses.<\/jats:p>","DOI":"10.1093\/immhor\/vlaf051","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T04:52:47Z","timestamp":1762750367000},"source":"Crossref","is-referenced-by-count":4,"title":["Shared and distinct responses of human and murine alveolar macrophages and monocyte-derived macrophages to\n                    <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1093","volume":"9","author":[{"given":"Kimberly A","family":"Dill-McFarland","sequence":"first","affiliation":[{"name":"Allergy and Infectious Diseases, Department of Medicine, University of Washington , Seattle, WA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenna","family":"Peterson","sequence":"additional","affiliation":[{"name":"Allergy and Infectious Diseases, Department of Medicine, University of Washington , Seattle, WA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1905-8785","authenticated-orcid":false,"given":"Pamelia N","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]},{"name":"Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn","family":"Skerrett","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington , Seattle, WA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R","family":"Hawn","sequence":"additional","affiliation":[{"name":"Allergy and Infectious Diseases, Department of Medicine, University of Washington , Seattle, WA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7484-1193","authenticated-orcid":false,"given":"Alissa C","family":"Rothchild","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4336-0519","authenticated-orcid":false,"given":"Monica","family":"Campo","sequence":"additional","affiliation":[{"name":"Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Minnesota , Minneapolis, MN,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,10,9]]},"reference":[{"key":"2025111000470482600_vlaf051-B1","author":"World Health Organization","year":"2023"},{"key":"2025111000470482600_vlaf051-B2","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.aaw6693","article-title":"Alveolar macrophages generate a noncanonical NRF2-driven transcriptional response to Mycobacterium tuberculosis in vivo","volume":"4","author":"Rothchild","year":"2019","journal-title":"Sci Immunol"},{"key":"2025111000470482600_vlaf051-B3","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.chom.2018.08.001","article-title":"Alveolar macrophages provide an early Mycobacterium tuberculosis niche and initiate dissemination","volume":"24","author":"Cohen","year":"2018","journal-title":"Cell Host 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