{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:30:09Z","timestamp":1793727009904,"version":"4.6.1"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":28,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Sciences and Engineering Research Council of Canada","id":[{"id":"https:\/\/ror.org\/01h531d29","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Canada Foundation for Innovation, the Ontario Research Fund, and Brock University","id":[{"id":"https:\/\/ror.org\/056am2717","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NSERC Master\u2019s Canada Graduate Scholarship"},{"name":"NSERC Doctoral Canada Graduate Scholarship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mast cells are critical players in the maladaptive immune responses underlying biphasic reactions in allergic inflammation. Neurogranin (Ng) is an IQ domain\u2013containing protein that sequesters Calmodulin under low [Ca2+] and negatively regulates calmodulin-mediated signaling such as calcineurin activation, in addition to downstream inflammatory responses. Previously described as brain specific, Ng has recently been identified in the spleen, bone marrow, and B lymphocytes, highlighting the need to investigate its negative regulatory role in other proinflammatory contexts. Here, we sought to determine the role of Ng in allergen-activated mast cells, to better understand the negative regulatory mechanisms that can potentially be exploited to help alleviate the severity of allergic inflammation. Using bone marrow\u2013derived mast cells from wild-type (Nrgn+\/+) and heterozygous (Nrgn+\/\u2212) mice, we identified that Ng is present in mast cells. It was determined that although Ng did not influence the development of mature mast cells or the extent of early-phase inflammation, a reduction in Ng significantly increased gene expression of IL6 and IL13, which was coupled with an increase in release of IL-6, IL-13, TNF, CCL1, CCL2, and CCL3. Together, this is the first study to identify Ng in mast cells, in addition to positioning its role as a negative regulator of mast cell responses following allergen activation. These data highlight the need for future research to further elucidate the role of Ng in IgE-mediated mast cell activation to better understand the regulatory mechanisms of these inflammatory cells in mast cell\u2013driven normal and pathological contexts.<\/jats:p>","DOI":"10.1093\/immhor\/vlag026","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:58:57Z","timestamp":1780016337000},"source":"Crossref","is-referenced-by-count":0,"title":["Neurogranin negatively regulates gene expression and proinflammatory mediator release in allergen-activated mast cells"],"prefix":"10.1093","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9219-0232","authenticated-orcid":false,"given":"Katie D","family":"Hunter","sequence":"first","affiliation":[{"name":"Department of Health Sciences, Faculty of Applied Health Sciences, Cairns Family Health and Bioscience Research Complex, Brock University , Niagara Region, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2146-5769","authenticated-orcid":false,"given":"Robert W E","family":"Crozier","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Faculty of Applied Health Sciences, Cairns Family Health and Bioscience Research Complex, Brock University , Niagara Region, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan W","family":"Baranowski","sequence":"additional","affiliation":[{"name":"Department of Kinesiology, Faculty of Applied Health Sciences, Cairns Family Health and Bioscience Research Complex, Brock University , Niagara Region, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val A","family":"Fajardo","sequence":"additional","affiliation":[{"name":"Department of Kinesiology, Faculty of Applied Health Sciences, Cairns Family Health and Bioscience Research Complex, Brock University , Niagara Region, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4749-8120","authenticated-orcid":false,"given":"Adam J","family":"MacNeil","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Faculty of Applied Health Sciences, Cairns Family Health and Bioscience Research Complex, Brock University , Niagara Region, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"2026110311363282100_vlag026-B1","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1002\/eji.201040559","article-title":"Mast cells in allergy and infection: versatile effector and regulatory cells in innate and acquired immunity","volume":"40","author":"Galli","year":"2010","journal-title":"Eur J 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