{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T20:29:37Z","timestamp":1793737777292,"version":"4.6.1"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":9,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["22K20868"],"award-info":[{"award-number":["22K20868"]}]},{"name":"JSPS KAKENHI","award":["24K11056"],"award-info":[{"award-number":["24K11056"]}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT) Leading Initiative for Excellent Young Researchers","id":[{"id":"https:\/\/ror.org\/048rj2z13","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100011907","name":"Mishima Kaiun Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011907","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Kanehara Ichiro Memorial Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Regulatory T cells (Tregs) maintain immune homeostasis in vivo. Similar to conventional T cells (Tconvs), Tregs are divided into na\u00efve and memory cells. Tregs have unique metabolic properties, including enhanced oxidative phosphorylation. The lipidomic profiles of human Tregs have been studied previously; however, those of na\u00efve and memory Tregs have not yet been consistently compared. Thus, in the present study, we used a combined transcriptomic and lipidomic analysis to assess the metabolic features of human na\u00efve and memory Tregs upon activation. Using transcriptomic analysis, we identified distinct gene expression profiles in na\u00efve and memory Tregs compared with those in Tconvs. Upon TCR stimulation, memory Tregs showed a lipidomic profile distinct from that of memory Tconvs, whereas the lipidomic profiles of na\u00efve Tregs were similar to those of na\u00efve Tconvs. Furthermore, upon TCR stimulation, memory Tregs expressed triglycerides (TGs) that were more enriched in monounsaturated fatty acids and polyunsaturated fatty acids (PUFAs) than those of memory Tconvs. However, memory Tconvs also had higher PUFA-TG levels than na\u00efve Tconvs and Tregs after TCR stimulation, although their levels remained lower than those in memory Tregs. These findings suggest PUFA-TGs could be a marker of memory Tregs. In turn, higher frequency of memory cells within the Treg population may also contribute to the observed enrichment of PUFA-TGs in Tregs upon TCR activation. Our study demonstrated unique transcriptomic and lipidomic profiles and enhanced lipogenesis in memory Tregs upon TCR stimulation.<\/jats:p>","DOI":"10.1093\/immhor\/vlag035","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:35:54Z","timestamp":1783690554000},"source":"Crossref","is-referenced-by-count":0,"title":["Combined transcriptomic and lipidomic analysis reveals enhanced lipogenesis in memory Tregs upon TCR activation"],"prefix":"10.1093","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8042-0069","authenticated-orcid":false,"given":"Yohei","family":"Sato","sequence":"first","affiliation":[{"name":"Laboratory of Immune Cell Therapy, Project Research Units, Research Center for Medical Sciences, The Jikei University School of Medicine , Tokyo,","place":["Japan"]},{"name":"Core Research Facility, Research Center for Medical Sciences, The Jikei University School of Medicine , Tokyo,","place":["Japan"]},{"name":"Immunology and Allergy Research Unit, Department of Otorhinolaryngology Head & Neck Surgery, Faculty of Medical Sciences, University of Fukui , Fukui,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"2026110314570743200_vlag035-B1","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.4049\/jimmunol.155.3.1151","article-title":"Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). 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