{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:05:02Z","timestamp":1790665502953,"version":"4.1.0"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T00:00:00Z","timestamp":1595635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Medical Scientific Research Foundation of Guangdong Province, China","award":["A2018264"],"award-info":[{"award-number":["A2018264"]}]},{"name":"Medical Scientific Research Foundation of Guangdong Province, 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Agency of the Czech Republic","doi-asserted-by":"publisher","award":["GA17-25455S"],"award-info":[{"award-number":["GA17-25455S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"name":"American Society for Tropical Medicine and Hygiene-Burroughs Wellcome Foundation Award"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Whole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiaa449","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T15:15:43Z","timestamp":1595430943000},"page":"848-853","source":"Crossref","is-referenced-by-count":32,"title":["Analysis of\n                    <i>Treponema pallidum<\/i>\n                    Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres"],"prefix":"10.1093","volume":"223","author":[{"given":"Wentao","family":"Chen","sequence":"first","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"\u0160majs","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfei","family":"Hu","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wujian","family":"Ke","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Posp\u00ed\u0161ilov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly L","family":"Hawley","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Department of Pediatrics, Connecticut Children\u2019s Medical Center, Hartford, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa J","family":"Caimano","sequence":"additional","affiliation":[{"name":"Departments of Medicine, Pediatrics, and Molecular Biology and Biophysics, UConn Health, Farmington, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin D","family":"Radolf","sequence":"additional","affiliation":[{"name":"Departments of Medicine, Pediatrics, and Molecular Biology and Biophysics, UConn Health, Farmington, Connecticut, USA"},{"name":"Departments of Genetics and Genome Sciences, and Immunology, UConn Health, Farmington, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlene","family":"Sena","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph D","family":"Tucker","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA"},{"name":"Faculty of Infectious and Tropical Diseases, London School 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