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Our goals were to confirm infection, identify species, and analyze biogeographical origin of metacestode tissues from a suspected human AE case in Saskatchewan, Canada. We conducted polymerase chain reaction (PCR) targeting the nad1 mitochondrial gene for E. multilocularis and the rrnS ribosomal RNA gene for E. granulosus and conducted haplotype analysis at the nad2 locus. Our analysis confirmed AE and indicated that sequences matched infected Saskatchewan coyotes and European E3\/E4 haplotypes. The patient had no travel history outside North America. This suggests autochthonous transmission of a European-type strain.<\/jats:p>","DOI":"10.1093\/infdis\/jiaa473","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T19:13:27Z","timestamp":1596568407000},"page":"1015-1018","source":"Crossref","is-referenced-by-count":14,"title":["Molecular Evidence for Local Acquisition of Human Alveolar Echinococcosis in Saskatchewan, Canada"],"prefix":"10.1093","volume":"223","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6122-5332","authenticated-orcid":false,"given":"Janna M","family":"Schurer","sequence":"first","affiliation":[{"name":"Center for One Health, University of Global Health Equity, Kigali, Rwanda"},{"name":"Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polly","family":"Tsybina","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Saskatchewan, Saskatoon, 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