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Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80\u00b0C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels.<\/jats:p>","DOI":"10.1093\/infdis\/jiaa507","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T15:15:58Z","timestamp":1596726958000},"page":"1462-1467","source":"Crossref","is-referenced-by-count":230,"title":["Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays"],"prefix":"10.1093","volume":"222","author":[{"given":"Edward I","family":"Patterson","sequence":"first","affiliation":[{"name":"Department of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Disease, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessa","family":"Prince","sequence":"additional","affiliation":[{"name":"National Institute for Health Research Health Protection Unit in Emerging and Zoonotic 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