{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T11:44:22Z","timestamp":1771328662823,"version":"3.50.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"Supplement_2","license":[{"start":{"date-parts":[[2021,8,15]],"date-time":"2021-08-15T00:00:00Z","timestamp":1628985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Auburn University Molecular Diagnostics Laboratory","award":["107"],"award-info":[{"award-number":["107"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Detection of anti-Chlamydia trachomatis (Ctr) antibodies is compromised by cross-reactivity and poor sensitivity of classic Ctr-antigens. We discovered 48 strongly reactive peptide antigens of Ctr-specific B-cell epitopes from 21 immunodominant proteins. In this study, we review the utility of peptide assays for diagnosis of Ctr infections. By combining many of these Ctr-specific B-cell epitopes from several proteins in separate or mixed multipeptide assays, they achieved vastly superior assay sensitivity and specificity over standard enzyme-linked immunosorbent assays. Such multipeptide assays eliminate cross-reactivities (false positives) and correct for stochastic gaps in antibody responses (false negatives). More importantly, we developed and validated a novel microarray platform in which hundreds of peptides from many proteins are spotted in a single reaction well. This offers the possibility of high-throughput screening of many candidate peptides for routine serological fingerprinting of Ctr infections. Discovery of optimal sets of antibody responses that associate with clinical pelvic inflammatory disease (PID) may identify diagnostically useful PID biomarker antigens.<\/jats:p>","DOI":"10.1093\/infdis\/jiab016","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T22:45:47Z","timestamp":1610664347000},"page":"S86-S95","source":"Crossref","is-referenced-by-count":10,"title":["Multipeptide Assays for Sensitive and Differential Detection of Anti-<i>Chlamydia Trachomatis<\/i> Antibodies"],"prefix":"10.1093","volume":"224","author":[{"given":"Kh Shamsur","family":"Rahman","sequence":"first","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"Kaltenboeck","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,8,16]]},"reference":[{"key":"2021081606011169800_CIT0001","first-page":"1","article-title":"Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae - 2014","volume":"63","author":"Centers for Disease Control and Prevention","year":"2014","journal-title":"MMWR Recomm Rep"},{"key":"2021081606011169800_CIT0002","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1128\/CMR.10.1.160","article-title":"Current methods of laboratory diagnosis of Chlamydia trachomatis infections","volume":"10","author":"Black","year":"1997","journal-title":"Clin Microbiol Rev"},{"key":"2021081606011169800_CIT0003","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1574-695X.2008.00526.x","article-title":"Typing Chlamydia trachomatis: from egg yolk to nanotechnology","volume":"55","author":"Pedersen","year":"2009","journal-title":"FEMS Immunol Med Microbiol"},{"key":"2021081606011169800_CIT0004","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3389\/fcimb.2013.00099","article-title":"Laboratory diagnosis of persistent human chlamydial infection","volume":"3","author":"Puolakkainen","year":"2013","journal-title":"Front Cell Infect Microbiol"},{"key":"2021081606011169800_CIT0005","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0732-8893(01)00299-1","article-title":"Cross-reaction between the genus-specific lipopolysaccharide antigen of Chlamydia spp. and the lipopolysaccharides of Porphyromonas gingivalis, Escherichia coli O119 and Salmonella newington: implications for diagnosis","volume":"41","author":"Haralambieva","year":"2001","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2021081606011169800_CIT0006","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1128\/JCM.39.4.1368-1377.2001","article-title":"Chlamydial serology: comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens","volume":"39","author":"Bas","year":"2001","journal-title":"J Clin Microbiol"},{"key":"2021081606011169800_CIT0007","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1139\/m92-194","article-title":"Specificity of the microimmunofluorescence assay for the serodiagnosis of Chlamydia pneumoniae infections","volume":"38","author":"Ozanne","year":"1992","journal-title":"Can J Microbiol"},{"key":"2021081606011169800_CIT0008","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1378\/chest.104.1.208","article-title":"A seroepidemiologic study of Chlamydia pneumoniae in Rhode Island. 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