{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T06:49:07Z","timestamp":1759042147246},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T00:00:00Z","timestamp":1628294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"R\u00e9gion Centre-Val de Loire"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Maternal-fetal transmission of Toxoplasma gondii tachyzoites acquired during pregnancy has potentially dramatic consequences for the fetus. Current reference-standard treatments are not specific to the parasite and can induce severe side effects. In order to provide treatments with a higher specificity against toxoplasmosis, we developed antibody fragments\u2014single-chain fragment variable (scFv) and scFv fused with mouse immunoglobulin G2a crystallizable fragment (scFv-Fc)\u2014directed against the major surface protein SAG1. After validating their capacity to inhibit T. gondii proliferation in vitro, the antibody fragments\u2019 biological activity was assessed in vivo using a congenital toxoplasmosis mouse model. Dams were treated by systemic administration of antibody fragments and with prevention of maternal-fetal transmission being used as the parameter of efficacy. We observed that both antibody fragments prevented T. gondii dissemination and protected neonates, with the scFv-Fc format having better efficacy. These data provide a proof of concept for the use of antibody fragments as effective and specific treatment against congenital toxoplasmosis and provide promising leads.<\/jats:p>","DOI":"10.1093\/infdis\/jiab141","type":"journal-article","created":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T20:09:53Z","timestamp":1615579793000},"page":"705-714","source":"Crossref","is-referenced-by-count":6,"title":["Engineering and Functional Evaluation of Neutralizing Antibody Fragments Against Congenital Toxoplasmosis"],"prefix":"10.1093","volume":"224","author":[{"given":"Anne","family":"di Tommaso","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Tours, Institut national de recherche pour l\u2019agriculture, l\u2019alimentation et l\u2019environnement (INRAE), Unit\u00e9 mixte de recherche 1282 (UMR1282), Infectiologie et sant\u00e9 publique (ISP), Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthieu O","family":"Juste","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, Institut national de recherche pour l\u2019agriculture, l\u2019alimentation et l\u2019environnement (INRAE), Unit\u00e9 mixte de recherche 1282 (UMR1282), Infectiologie et sant\u00e9 publique (ISP), Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zineb","family":"Lakhrif","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, Institut national de recherche pour l\u2019agriculture, l\u2019alimentation et l\u2019environnement (INRAE), Unit\u00e9 mixte de recherche 1282 (UMR1282), Infectiologie et sant\u00e9 publique (ISP), Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-No\u00eblle","family":"M\u00e9v\u00e9lec","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, Institut national de recherche pour l\u2019agriculture, l\u2019alimentation et l\u2019environnement (INRAE), Unit\u00e9 mixte de recherche 1282 (UMR1282), Infectiologie et sant\u00e9 publique (ISP), Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coraline","family":"Borowczyk","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, Institut national de recherche pour l\u2019agriculture, l\u2019alimentation et l\u2019environnement (INRAE), Unit\u00e9 mixte de recherche 1282 (UMR1282), Infectiologie et sant\u00e9 publique (ISP), Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Hammeni","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, Institut national de recherche pour l\u2019agriculture, l\u2019alimentation et l\u2019environnement (INRAE), Unit\u00e9 mixte de recherche 1282 (UMR1282), Infectiologie et sant\u00e9 publique (ISP), Tours, 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