{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:41:33Z","timestamp":1761396093910,"version":"3.37.3"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T00:00:00Z","timestamp":1627603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse-transcription polymerase chain reaction (RT-PCR) provides a highly variable cycle threshold (Ct) value that cannot distinguish viral infectivity. Subgenomic ribonucleic acid (sgRNA) has been used to monitor active replication. Given the importance of long RT-PCR positivity and the need for work reincorporation and discontinuing isolation, we studied the functionality of normalized viral loads (NVLs) for patient monitoring and sgRNA for viral infectivity detection.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The NVLs measured through the Nucleocapsid and RNA-dependent-RNA-polymerase genes and sgRNA RT-PCRs were performed in 2 consecutive swabs from 84 healthcare workers.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The NVLs provided similar and accurate quantities of both genes of SARS-CoV-2 at 2 different timepoints of infection, overcoming Ct-value and swab collection variability. Among SARS-CoV-2-positive samples, 51.19% were sgRNA-positive in the 1st RT-PCR and 5.95% in the 2nd RT-PCR. All sgRNA-positive samples had &amp;gt;4 log10 RNA copies\/1000 cells, whereas samples with \u22641 log10 NVLs were sgRNA-negative. Although NVLs were positive until 29 days after symptom onset, 84.1% of sgRNA-positive samples were from the first 7 days, which correlated with viral culture viability. Multivariate analyses showed that sgRNA, NVLs, and days of symptoms were significantly associated (P\u2005&amp;lt;\u2005.001).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The NVLs and sgRNA are 2 rapid accessible techniques that could be easily implemented in routine hospital practice providing a useful proxy for viral infectivity and<\/jats:p>\n                  <jats:p>coronavirus disease 2019 patient follow-up.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiab394","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T19:08:39Z","timestamp":1627585719000},"page":"1325-1332","source":"Crossref","is-referenced-by-count":18,"title":["Severe Acute Respiratory Syndrome Coronavirus 2 Normalized Viral Loads and Subgenomic RNA Detection as Tools for Improving Clinical Decision Making and Work Reincorporation"],"prefix":"10.1093","volume":"224","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5270-4454","authenticated-orcid":false,"given":"Marta","family":"Santos Bravo","sequence":"first","affiliation":[{"name":"Department of Microbiology, Hospital Cl\u00ednic of Barcelona, Institut of Global Health (ISGlobal), Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Nicol\u00e1s","sequence":"additional","affiliation":[{"name":"Hospital at Home Unit, Internal Medicine Service, Hospital Cl\u00ednic Barcelona, University of Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Berengua","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cl\u00ednic of Barcelona, Institut of Global Health (ISGlobal), Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Carlos","family":"Hurtado","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cl\u00ednic of Barcelona, Institut of Global Health (ISGlobal), Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Tortajada","sequence":"additional","affiliation":[{"name":"Risk Prevention Service, Hospital Cl\u00ednic of Barcelona, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Barroso","sequence":"additional","affiliation":[{"name":"Risk Prevention Service, Hospital Cl\u00ednic of Barcelona, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Vilella","sequence":"additional","affiliation":[{"name":"Epidemiology and Preventive Medicine Service, Hospital Cl\u00ednic of Barcelona, Institut of Global Health (ISGlobal), Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Mar","family":"Mosquera","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cl\u00ednic of Barcelona, Institut of Global Health (ISGlobal), Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Vila","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cl\u00ednic of Barcelona, Institut of Global Health (ISGlobal), Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Angeles","family":"Marcos","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cl\u00ednic of Barcelona, Institut of Global Health (ISGlobal), Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,7,30]]},"reference":[{"key":"2021103113412086800_CIT0001","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1056\/NEJMoa2001017","article-title":"A novel coronavirus from patients with pneumonia in China, 2019","volume":"382","author":"Zhu","year":"2020","journal-title":"N Engl J Med"},{"volume-title":"World Health Organization, Emergency Response. 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