{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:33:34Z","timestamp":1791254014296,"version":"4.1.0"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2021,8,28]],"date-time":"2021-08-28T00:00:00Z","timestamp":1630108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020"},{"name":"Marie Sklodowska-Curie","award":["665807"],"award-info":[{"award-number":["665807"]}]},{"name":"French Parasitology Consortium ParaFrap","award":["ANR-11-LABX0024"],"award-info":[{"award-number":["ANR-11-LABX0024"]}]},{"name":"Fondation Pasteur Suisse"},{"name":"Pasteur Cantarini-Roux"},{"name":"Fundac\u0327a\u0303o de Amparo a\u0300 Pesquisa do Estado de Sa\u0303o Paulo","award":["2016\/18740-9"],"award-info":[{"award-number":["2016\/18740-9"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.<\/jats:p>","DOI":"10.1093\/infdis\/jiab431","type":"journal-article","created":{"date-parts":[[2021,8,28]],"date-time":"2021-08-28T13:34:34Z","timestamp":1630157674000},"page":"1621-1625","source":"Crossref","is-referenced-by-count":5,"title":["Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species"],"prefix":"10.1093","volume":"225","author":[{"given":"Camilla","family":"Luiza-Batista","sequence":"first","affiliation":[{"name":"Biology of Host-Parasite Interactions Unit, Institut Pasteur , Paris , France"},{"name":"INSERM U1201 , Paris , France"},{"name":"CNRS ERL9195 , Paris , France"},{"name":"Sorbonne Universit\u00e9, Ecole Doctorale Complexit\u00e9 du vivant , Paris , 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