{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T09:06:24Z","timestamp":1740128784117,"version":"3.37.3"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI113103","K24 AG042489"],"award-info":[{"award-number":["R01AI113103","K24 AG042489"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Von Zedtwitz Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chlamydia trachomatis serovars A\u2013L cause important diseases of the eyes and reproductive tract by infecting epithelium lining those organs. A major hurdle for vaccine trials is finding a surrogate biomarker for protective immunity. Investigational data argues for T-cell biomarker(s) reflecting mucosal adaption, cytokine polarization, B-cell help, antibacterial effector mechanisms, or some combination thereof. A human investigation and 2 mouse studies link IL-13 to protection from infection\/immunopathology. We performed RNAseq on T cells resident in spleens and genital tracts of naturally immune mice. CD4 signatures were consistent with helper function that differed by site including a genital tract-specific Fgl2 signal. The genital tract CD8 signature featured IL-10 and promotion of healing\/scarring with a unique transcription of granzyme A. The RNAseq data was used to refine previously published CD4\u03b313 and CD8\u03b313 transcriptomes derived from protective T-cell clones, potentially identifying practicable T-cell subset signatures for assessing Chlamydia vaccine candidates.<\/jats:p>","DOI":"10.1093\/infdis\/jiac051","type":"journal-article","created":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T20:12:18Z","timestamp":1644869538000},"page":"2033-2042","source":"Crossref","is-referenced-by-count":1,"title":["Combining Cellular Immunology With RNAseq to Identify Novel Chlamydia T-Cell Subset Signatures"],"prefix":"10.1093","volume":"225","author":[{"given":"Raymond M","family":"Johnson","sequence":"first","affiliation":[{"name":"Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine , New Haven, Connecticut , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromitsu","family":"Asashima","sequence":"additional","affiliation":[{"name":"Department of 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