{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T13:00:21Z","timestamp":1754398821754,"version":"3.37.3"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"4","funder":[{"name":"PATH MACEPA"},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"crossref","award":["BMGF OPP-00069818"],"award-info":[{"award-number":["BMGF OPP-00069818"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005624","name":"PATH","doi-asserted-by":"crossref","award":["AWD-451218"],"award-info":[{"award-number":["AWD-451218"]}],"id":[{"id":"10.13039\/100005624","id-type":"DOI","asserted-by":"crossref"}]},{"name":"US Centers for Diseases Control and Prevention","award":["5U01GH001646"],"award-info":[{"award-number":["5U01GH001646"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Screen-and-treat strategies with sensitive diagnostic tests may reduce malaria-associated adverse pregnancy outcomes. We conducted a diagnostic accuracy study to evaluate new point-of-care tests to screen pregnant women for malaria at their first antenatal visit in western Kenya.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Consecutively women were tested for Plasmodium infection by expert microscopy, conventional rapid diagnostic test (cRDT), ultra sensitive RDT (usRDT), and loop-mediated isothermal amplification (LAMP). Photoinduced electron-transfer polymerase chain reaction (PET-PCR) served as the reference standard. Diagnostic performance was calculated and modelled at low parasite densities.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Between May and September 2018, 172 of 482 screened participants (35.7%) were PET-PCR positive. Relative to PET-PCR, expert microscopy was least sensitive (40.1%; 95% confidence interval [CI], 32.7%\u201347.9%), followed by cRDT (49.4%; 95% CI, 41.7%\u201357.1), usRDT (54.7%; 95% CI, 46.9%\u201362.2%), and LAMP (68.6%; 95% CI, 61.1%\u201375.5%). Test sensitivities were comparable in febrile women (n\u2009=\u200990). Among afebrile women (n\u2009=\u2009392), the geometric-mean parasite density was 29\u2005parasites\/\u00b5L and LAMP (sensitivity\u2009=\u200961.9%) and usRDT (43.2%) detected 1.74 (95% CI, 1.31\u20132.30) and 1.21 (95% CI, 88\u20132.21) more infections than cRDT (35.6%). Per our model, tests performed similarly at densities &amp;gt;200\u2005parasites\/\u00b5L. At 50\u2005parasites\/\u00b5L, the sensitivities were 45%, 56%, 62%, and 74% with expert microscopy, cRDT, usRDT, and LAMP, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This first-generation usRDT provided moderate improvement in detecting low-density infections in afebrile pregnant women compared to cRDTs.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiac289","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T02:46:15Z","timestamp":1657507575000},"page":"696-707","source":"Crossref","is-referenced-by-count":7,"title":["Diagnostic Performance of Loop-Mediated Isothermal Amplification and Ultrasensitive Rapid Diagnostic Tests for Malaria Screening Among Pregnant Women in Kenya"],"prefix":"10.1093","volume":"226","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8355-8186","authenticated-orcid":false,"given":"Aaron M","family":"Samuels","sequence":"first","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention , Kisumu , Kenya"},{"name":"Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention , Atlanta, Georgia , USA"},{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine , Liverpool , United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Towett","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute , Kisumu , Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Seda","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute , Kisumu , Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan E","family":"Wiegand","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention , Atlanta, Georgia , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kephas","family":"Otieno","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute , Kisumu , Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Chomba","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute , Kisumu , Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Lucchi","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention , Atlanta, Georgia , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dragan","family":"Ljolje","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention , Atlanta, Georgia , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kammerle","family":"Schneider","sequence":"additional","affiliation":[{"name":"Center for Malaria Control and Elimination, PATH , Seattle, Washington , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick G T","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Epidemiology, Imperial College London , London , United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Titus K","family":"Kwambai","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention , Kisumu , Kenya"},{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine , Liverpool , United Kingdom"},{"name":"Centre for Global Health Research, Kenya Medical Research Institute , Kisumu , Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence","family":"Slutsker","sequence":"additional","affiliation":[{"name":"Center for Malaria Control and Elimination, PATH , Seattle, Washington , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feiko O","family":"ter Kuile","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine , Liverpool , United Kingdom"},{"name":"Centre for Global Health Research, Kenya Medical Research Institute , Kisumu , Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon K","family":"Kariuki","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute , Kisumu , 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