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Compared with the broth-grown inocula, transcription of the H. ducreyi ulaABCD operon, which encodes genes for ascorbic acid uptake, is increased in pustules. We hypothesized that ascorbic acid uptake plays a role in H. ducreyi virulence. Five volunteers were infected with both H. ducreyi strain 35000HP and its isogenic ulaABCD deletion mutant at multiple sites; the papule and pustule formation rates of the mutant and parent strains were similar. Thus, ascorbic acid uptake is not essential for H. ducreyi virulence in humans.<\/jats:p>","DOI":"10.1093\/infdis\/jiac314","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T14:18:36Z","timestamp":1658758716000},"page":"317-321","source":"Crossref","is-referenced-by-count":7,"title":["Dispensability of Ascorbic Acid Uptake and Utilization Encoded by <i>ulaABCD<\/i> for the Virulence of <i>Haemophilus ducreyi<\/i> in Humans"],"prefix":"10.1093","volume":"227","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5277-3692","authenticated-orcid":false,"given":"Julie A","family":"Brothwell","sequence":"first","affiliation":[{"name":"Departments of Microbiology and Immunology, Indiana University , Indianapolis, Indiana , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate R","family":"Fortney","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, Indiana University , Indianapolis, Indiana , 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