{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T13:48:31Z","timestamp":1780840111352,"version":"3.54.1"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T00:00:00Z","timestamp":1666656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Center for Childhood Infections and Vaccines"},{"DOI":"10.13039\/100005886","name":"Pediatric Infectious Diseases Society","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100005886","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Pichichero Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,12,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Respiratory syncytial virus (RSV) is a leading viral respiratory pathogen in infants. The objective of this study was to generate RSV live-attenuated vaccine (LAV) candidates by removing the G-protein mucin domains to attenuate viral replication while retaining immunogenicity through deshielding of surface epitopes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Two LAV candidates were generated from recombinant RSV A2-line19F by deletion of the G-protein mucin domains (A2-line19F-G155) or deletion of the G-protein mucin and transmembrane domains (A2-line19F-G155S). Vaccine attenuation was measured in BALB\/c mouse lungs by fluorescent focus unit (FFU) assays and real-time polymerase chain reaction (RT-PCR). Immunogenicity was determined by measuring serum binding and neutralizing antibodies in mice following prime\/boost on days 28 and 59. Efficacy was determined by measuring RSV lung viral loads on day 4 postchallenge.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Both LAVs were undetectable in mouse lungs by FFU assay and elicited similar neutralizing antibody titers compared to A2-line19F on days 28 and 59. Following RSV challenge, vaccinated mice showed no detectable RSV in the lungs by FFU assay and a significant reduction in RSV RNA in the lungs by RT-PCR of 560-fold for A2-line19F-G155 and 604-fold for A2-line19F-G155S compared to RSV-challenged, unvaccinated mice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Removal of the G-protein mucin domains produced RSV LAV candidates that were highly attenuated with retained immunogenicity.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiac382","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T21:33:59Z","timestamp":1666388039000},"page":"50-60","source":"Crossref","is-referenced-by-count":8,"title":["An RSV Live-Attenuated Vaccine Candidate Lacking G Protein Mucin Domains Is Attenuated, Immunogenic, and Effective in Preventing RSV in BALB\/c Mice"],"prefix":"10.1093","volume":"227","author":[{"given":"Molly K","family":"Roe","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Butler University , Indianapolis, Indiana , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A","family":"Perez","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Emory University School of Medicine , Atlanta, Georgia , USA"},{"name":"Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Mien","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Emory University School of Medicine , Atlanta, Georgia , USA"},{"name":"Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey A","family":"Lapp","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Emory University School of Medicine , Atlanta, Georgia , USA"},{"name":"Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He-Ying","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Emory University School of Medicine , Atlanta, Georgia , USA"},{"name":"Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samadhan","family":"Jadhao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Emory University School of Medicine , Atlanta, Georgia , USA"},{"name":"Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey R","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Butler University , Indianapolis, Indiana , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yara S","family":"Batista","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Butler University , Indianapolis, Indiana , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan C","family":"Reed","sequence":"additional","affiliation":[{"name":"Department of Medicine, Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azmain","family":"Taz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5942-1534","authenticated-orcid":false,"given":"Anne","family":"Piantadosi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Emory University School of Medicine , Atlanta, Georgia , USA"},{"name":"Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Koval","sequence":"additional","affiliation":[{"name":"Department of Medicine, Emory University School of Medicine , Atlanta, Georgia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy A","family":"Snider","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Oklahoma State University , Stillwater, Oklahoma , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin L","family":"Moore","sequence":"additional","affiliation":[{"name":"Meissa Vaccines, Inc , Redwood City, California , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1576-4420","authenticated-orcid":false,"given":"Evan J","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Emory University School of Medicine , Atlanta, Georgia , USA"},{"name":"Center for Childhood 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